Ubiquitin-conjugating enzyme family protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IFB8_LEIDO

TriTrypDb:

LdBPK_320730.1 , LdCL_320012900 , LDHU3_32.0910

Length:

234

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 14
NetGPI	no	yes: 0, no: 14

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

A0A3Q8IFB8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IFB8

PDB structure(s): 1yf9_A , 1yf9_B , 1yf9_C

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	2
GO:0006508	proteolysis	4	2
GO:0006511	ubiquitin-dependent protein catabolic process	7	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009987	cellular process	1	2
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	2
GO:0019941	modification-dependent protein catabolic process	6	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043632	modification-dependent macromolecule catabolic process	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0051603	proteolysis involved in protein catabolic process	5	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901575	organic substance catabolic process	3	2
GO:0007346	regulation of mitotic cell cycle	5	1
GO:0010498	proteasomal protein catabolic process	5	1
GO:0010564	regulation of cell cycle process	5	1
GO:0010965	regulation of mitotic sister chromatid separation	6	1
GO:0030071	regulation of mitotic metaphase/anaphase transition	7	1
GO:0030163	protein catabolic process	4	1
GO:0031145	anaphase-promoting complex-dependent catabolic process	7	1
GO:0033043	regulation of organelle organization	5	1
GO:0033044	regulation of chromosome organization	6	1
GO:0033045	regulation of sister chromatid segregation	5	1
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051128	regulation of cellular component organization	4	1
GO:0051726	regulation of cell cycle	4	1
GO:0051983	regulation of chromosome segregation	4	1
GO:0065007	biological regulation	1	1
GO:1901565	organonitrogen compound catabolic process	4	1
GO:1901987	regulation of cell cycle phase transition	6	1
GO:1901990	regulation of mitotic cell cycle phase transition	6	1
GO:1902099	regulation of metaphase/anaphase transition of cell cycle	6	1
GO:1905818	regulation of chromosome separation	5	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	15
GO:0003824	catalytic activity	1	14
GO:0005488	binding	1	15
GO:0005524	ATP binding	5	15
GO:0016740	transferase activity	2	13
GO:0017076	purine nucleotide binding	4	15
GO:0030554	adenyl nucleotide binding	5	15
GO:0032553	ribonucleotide binding	3	15
GO:0032555	purine ribonucleotide binding	4	15
GO:0032559	adenyl ribonucleotide binding	5	15
GO:0035639	purine ribonucleoside triphosphate binding	4	15
GO:0036094	small molecule binding	2	15
GO:0043167	ion binding	2	15
GO:0043168	anion binding	3	15
GO:0097159	organic cyclic compound binding	2	15
GO:0097367	carbohydrate derivative binding	2	15
GO:1901265	nucleoside phosphate binding	3	15
GO:1901363	heterocyclic compound binding	2	15
GO:0004842	ubiquitin-protein transferase activity	4	3
GO:0016874	ligase activity	2	6
GO:0019787	ubiquitin-like protein transferase activity	3	3
GO:0061631	ubiquitin conjugating enzyme activity	5	3
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	3
GO:0140096	catalytic activity, acting on a protein	2	3
GO:0016746	acyltransferase activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	183	187	PF00656	0.682
CLV_NRD_NRD_1	12	14	PF00675	0.320
CLV_NRD_NRD_1	24	26	PF00675	0.237
CLV_PCSK_KEX2_1	11	13	PF00082	0.330
CLV_PCSK_PC7_1	8	14	PF00082	0.473
CLV_PCSK_SKI1_1	195	199	PF00082	0.586
DOC_MAPK_gen_1	11	20	PF00069	0.397
DOC_MAPK_MEF2A_6	84	92	PF00069	0.402
DOC_PP1_RVXF_1	134	140	PF00149	0.265
DOC_PP4_FxxP_1	40	43	PF00568	0.235
DOC_PP4_FxxP_1	65	68	PF00568	0.272
DOC_USP7_MATH_1	118	122	PF00917	0.254
DOC_USP7_MATH_1	156	160	PF00917	0.357
DOC_USP7_MATH_1	191	195	PF00917	0.660
DOC_WW_Pin1_4	149	154	PF00397	0.385
DOC_WW_Pin1_4	200	205	PF00397	0.587
DOC_WW_Pin1_4	43	48	PF00397	0.337
DOC_WW_Pin1_4	64	69	PF00397	0.272
DOC_WW_Pin1_4	95	100	PF00397	0.235
LIG_14-3-3_CanoR_1	19	29	PF00244	0.270
LIG_BIR_II_1	1	5	PF00653	0.579
LIG_BRCT_BRCA1_1	68	72	PF00533	0.235
LIG_CSL_BTD_1	96	99	PF09270	0.235
LIG_deltaCOP1_diTrp_1	35	40	PF00928	0.235
LIG_FHA_1	22	28	PF00498	0.310
LIG_FHA_1	50	56	PF00498	0.287
LIG_FHA_2	168	174	PF00498	0.723
LIG_FHA_2	181	187	PF00498	0.599
LIG_FHA_2	201	207	PF00498	0.443
LIG_FHA_2	30	36	PF00498	0.366
LIG_FHA_2	44	50	PF00498	0.250
LIG_LIR_Apic_2	39	43	PF02991	0.235
LIG_LIR_Apic_2	62	68	PF02991	0.272
LIG_LIR_Gen_1	104	115	PF02991	0.383
LIG_LIR_Gen_1	35	43	PF02991	0.262
LIG_LIR_Nem_3	104	110	PF02991	0.418
LIG_LIR_Nem_3	14	20	PF02991	0.317
LIG_LIR_Nem_3	35	40	PF02991	0.291
LIG_PDZ_Class_2	229	234	PF00595	0.505
LIG_Pex14_1	97	101	PF04695	0.287
LIG_Pex14_2	36	40	PF04695	0.235
LIG_SH2_CRK	17	21	PF00017	0.347
LIG_SH2_SRC	48	51	PF00017	0.255
LIG_SH3_1	117	123	PF00018	0.291
LIG_SH3_3	106	112	PF00018	0.284
LIG_SH3_3	117	123	PF00018	0.327
LIG_SH3_3	54	60	PF00018	0.294
LIG_TRAF2_1	170	173	PF00917	0.643
MOD_CDK_SPxK_1	149	155	PF00069	0.388
MOD_CK1_1	200	206	PF00069	0.594
MOD_CK2_1	156	162	PF00069	0.480
MOD_CK2_1	167	173	PF00069	0.549
MOD_CK2_1	200	206	PF00069	0.555
MOD_CK2_1	29	35	PF00069	0.250
MOD_CK2_1	43	49	PF00069	0.366
MOD_GlcNHglycan	1	4	PF01048	0.698
MOD_GlcNHglycan	82	87	PF01048	0.236
MOD_GSK3_1	145	152	PF00069	0.453
MOD_GSK3_1	191	198	PF00069	0.567
MOD_LATS_1	193	199	PF00433	0.578
MOD_N-GLC_1	21	26	PF02516	0.461
MOD_N-GLC_1	93	98	PF02516	0.235
MOD_NEK2_1	197	202	PF00069	0.659
MOD_NEK2_1	20	25	PF00069	0.388
MOD_PK_1	66	72	PF00069	0.235
MOD_PK_1	84	90	PF00069	0.235
MOD_Plk_1	198	204	PF00069	0.669
MOD_Plk_1	93	99	PF00069	0.235
MOD_Plk_4	49	55	PF00069	0.287
MOD_ProDKin_1	149	155	PF00069	0.388
MOD_ProDKin_1	200	206	PF00069	0.588
MOD_ProDKin_1	43	49	PF00069	0.337
MOD_ProDKin_1	64	70	PF00069	0.272
MOD_ProDKin_1	95	101	PF00069	0.235
MOD_SUMO_rev_2	35	43	PF00179	0.265
TRG_ENDOCYTIC_2	17	20	PF00928	0.345
TRG_ER_diArg_1	11	13	PF00400	0.445

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HXA7	Leptomonas seymouri	31%	87%
A0A0N1I1I8	Leptomonas seymouri	74%	100%
A0A0S4IJE0	Bodo saltans	25%	100%
A0A0S4KIJ6	Bodo saltans	25%	94%
A0A1X0NW44	Trypanosomatidae	57%	100%
A0A3S7WX16	Leishmania donovani	31%	90%
A0A3S7WYK3	Leishmania donovani	37%	100%
A0A422NJI9	Trypanosoma rangeli	58%	100%
A4HCA6	Leishmania braziliensis	31%	90%
A4HDS4	Leishmania braziliensis	33%	100%
A4HK50	Leishmania braziliensis	93%	100%
A4HZT8	Leishmania infantum	29%	100%
A4I122	Leishmania infantum	37%	100%
A4I7L2	Leishmania infantum	100%	100%
C3Z724	Branchiostoma floridae	29%	100%
D0A9T7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	100%
D0A9W7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	95%
E9AVP3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9AX59	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E9B2J3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
E9B4B7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	97%
O00762	Homo sapiens	32%	100%
P49428	Komagataella pastoris	25%	100%
Q28F89	Xenopus tropicalis	28%	100%
Q32PA5	Bos taurus	31%	100%
Q4Q5L3	Leishmania major	98%	100%
Q4QAB7	Leishmania major	37%	100%
Q4QBT6	Leishmania major	29%	100%
Q4R9D1	Macaca fascicularis	32%	100%
Q9D1C1	Mus musculus	31%	100%
Q9NPD8	Homo sapiens	31%	100%
V5BAJ3	Trypanosoma cruzi	29%	99%