p1/s1 nuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

p1/s1 nuclease

Gene product:

p1/s1 nuclease (fragment)

Species:

Leishmania donovani

UniProt:

A0A3Q8IEW3_LEIDO

TriTrypDb:

LdBPK_301520.1 , LdCL_300020400 , LDHU3_30.1980

Length:

316

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 23, no: 6
NetGPI	no	yes: 0, no: 29

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10
GO:0009986	cell surface	2	1

Expansion

Sequence features

A0A3Q8IEW3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IEW3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	30
GO:0006259	DNA metabolic process	4	30
GO:0006308	DNA catabolic process	5	30
GO:0006725	cellular aromatic compound metabolic process	3	30
GO:0006807	nitrogen compound metabolic process	2	30
GO:0008152	metabolic process	1	30
GO:0009056	catabolic process	2	30
GO:0009057	macromolecule catabolic process	4	30
GO:0009987	cellular process	1	30
GO:0019439	aromatic compound catabolic process	4	30
GO:0034641	cellular nitrogen compound metabolic process	3	30
GO:0034655	nucleobase-containing compound catabolic process	4	30
GO:0043170	macromolecule metabolic process	3	30
GO:0044237	cellular metabolic process	2	30
GO:0044238	primary metabolic process	2	30
GO:0044248	cellular catabolic process	3	30
GO:0044260	obsolete cellular macromolecule metabolic process	3	30
GO:0044265	obsolete cellular macromolecule catabolic process	4	30
GO:0044270	cellular nitrogen compound catabolic process	4	30
GO:0046483	heterocycle metabolic process	3	30
GO:0046700	heterocycle catabolic process	4	30
GO:0071704	organic substance metabolic process	2	30
GO:0090304	nucleic acid metabolic process	4	30
GO:1901360	organic cyclic compound metabolic process	3	30
GO:1901361	organic cyclic compound catabolic process	4	30
GO:1901575	organic substance catabolic process	3	30
GO:0006163	purine nucleotide metabolic process	5	1
GO:0006195	purine nucleotide catabolic process	6	1
GO:0006196	AMP catabolic process	7	1
GO:0006753	nucleoside phosphate metabolic process	4	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0009117	nucleotide metabolic process	5	1
GO:0009123	nucleoside monophosphate metabolic process	5	1
GO:0009125	nucleoside monophosphate catabolic process	6	1
GO:0009126	purine nucleoside monophosphate metabolic process	6	1
GO:0009128	purine nucleoside monophosphate catabolic process	7	1
GO:0009150	purine ribonucleotide metabolic process	6	1
GO:0009154	purine ribonucleotide catabolic process	6	1
GO:0009158	ribonucleoside monophosphate catabolic process	7	1
GO:0009161	ribonucleoside monophosphate metabolic process	6	1
GO:0009166	nucleotide catabolic process	6	1
GO:0009167	purine ribonucleoside monophosphate metabolic process	7	1
GO:0009169	purine ribonucleoside monophosphate catabolic process	8	1
GO:0009259	ribonucleotide metabolic process	5	1
GO:0009261	ribonucleotide catabolic process	5	1
GO:0019637	organophosphate metabolic process	3	1
GO:0019693	ribose phosphate metabolic process	4	1
GO:0044281	small molecule metabolic process	2	1
GO:0046033	AMP metabolic process	7	1
GO:0046434	organophosphate catabolic process	4	1
GO:0055086	nucleobase-containing small molecule metabolic process	3	1
GO:0072521	purine-containing compound metabolic process	4	1
GO:0072523	purine-containing compound catabolic process	5	1
GO:1901135	carbohydrate derivative metabolic process	3	1
GO:1901136	carbohydrate derivative catabolic process	4	1
GO:1901292	nucleoside phosphate catabolic process	5	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901565	organonitrogen compound catabolic process	4	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	30
GO:0003824	catalytic activity	1	30
GO:0004518	nuclease activity	4	30
GO:0004519	endonuclease activity	5	30
GO:0005488	binding	1	30
GO:0016787	hydrolase activity	2	30
GO:0016788	hydrolase activity, acting on ester bonds	3	30
GO:0043167	ion binding	2	30
GO:0043169	cation binding	3	30
GO:0046872	metal ion binding	4	30
GO:0097159	organic cyclic compound binding	2	30
GO:1901363	heterocyclic compound binding	2	30
GO:0008252	nucleotidase activity	6	1
GO:0008254	3'-nucleotidase activity	7	1
GO:0016791	phosphatase activity	5	1
GO:0042578	phosphoric ester hydrolase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	53	57	PF00656	0.203
CLV_NRD_NRD_1	183	185	PF00675	0.400
CLV_NRD_NRD_1	44	46	PF00675	0.436
CLV_PCSK_FUR_1	270	274	PF00082	0.391
CLV_PCSK_FUR_1	311	315	PF00082	0.430
CLV_PCSK_KEX2_1	183	185	PF00082	0.385
CLV_PCSK_KEX2_1	272	274	PF00082	0.475
CLV_PCSK_KEX2_1	313	315	PF00082	0.703
CLV_PCSK_KEX2_1	44	46	PF00082	0.438
CLV_PCSK_KEX2_1	85	87	PF00082	0.459
CLV_PCSK_PC1ET2_1	272	274	PF00082	0.491
CLV_PCSK_PC1ET2_1	313	315	PF00082	0.460
CLV_PCSK_PC1ET2_1	85	87	PF00082	0.459
CLV_PCSK_SKI1_1	184	188	PF00082	0.529
DEG_APCC_DBOX_1	7	15	PF00400	0.403
DEG_SCF_FBW7_1	255	262	PF00400	0.203
DOC_CYCLIN_yCln2_LP_2	7	13	PF00134	0.635
DOC_MAPK_gen_1	180	188	PF00069	0.251
DOC_PP2B_LxvP_1	7	10	PF13499	0.405
DOC_PP4_FxxP_1	98	101	PF00568	0.191
DOC_USP7_MATH_1	67	71	PF00917	0.307
DOC_USP7_UBL2_3	50	54	PF12436	0.191
DOC_WW_Pin1_4	196	201	PF00397	0.206
DOC_WW_Pin1_4	255	260	PF00397	0.203
DOC_WW_Pin1_4	68	73	PF00397	0.303
LIG_14-3-3_CanoR_1	162	168	PF00244	0.384
LIG_14-3-3_CanoR_1	205	210	PF00244	0.258
LIG_14-3-3_CanoR_1	278	282	PF00244	0.269
LIG_14-3-3_CanoR_1	8	14	PF00244	0.546
LIG_APCC_ABBA_1	147	152	PF00400	0.187
LIG_BRCT_BRCA1_1	244	248	PF00533	0.324
LIG_BRCT_BRCA1_1	64	68	PF00533	0.200
LIG_DLG_GKlike_1	205	212	PF00625	0.203
LIG_eIF4E_1	284	290	PF01652	0.322
LIG_FHA_1	108	114	PF00498	0.309
LIG_FHA_1	24	30	PF00498	0.511
LIG_FHA_2	232	238	PF00498	0.359
LIG_FHA_2	260	266	PF00498	0.339
LIG_LIR_Apic_2	224	229	PF02991	0.261
LIG_LIR_Gen_1	208	217	PF02991	0.288
LIG_LIR_Gen_1	94	102	PF02991	0.287
LIG_LIR_Nem_3	208	212	PF02991	0.288
LIG_LIR_Nem_3	252	257	PF02991	0.233
LIG_LIR_Nem_3	94	98	PF02991	0.295
LIG_LYPXL_yS_3	254	257	PF13949	0.284
LIG_NRBOX	285	291	PF00104	0.289
LIG_SH2_CRK	203	207	PF00017	0.282
LIG_SH2_NCK_1	90	94	PF00017	0.190
LIG_SH2_STAP1	163	167	PF00017	0.324
LIG_SH2_STAP1	209	213	PF00017	0.203
LIG_SH2_STAT5	209	212	PF00017	0.282
LIG_SH2_STAT5	267	270	PF00017	0.205
LIG_SH2_STAT5	288	291	PF00017	0.321
LIG_SH3_3	66	72	PF00018	0.240
LIG_SH3_3	98	104	PF00018	0.252
LIG_SUMO_SIM_par_1	15	21	PF11976	0.524
LIG_SUMO_SIM_par_1	234	240	PF11976	0.175
LIG_TYR_ITIM	207	212	PF00017	0.283
LIG_TYR_ITSM	250	257	PF00017	0.191
LIG_UBA3_1	125	133	PF00899	0.217
LIG_WW_1	200	203	PF00397	0.203
LIG_WW_3	199	203	PF00397	0.287
MOD_CDK_SPxK_1	196	202	PF00069	0.194
MOD_CK1_1	161	167	PF00069	0.245
MOD_CK2_1	259	265	PF00069	0.270
MOD_Cter_Amidation	181	184	PF01082	0.403
MOD_GlcNHglycan	126	129	PF01048	0.512
MOD_GlcNHglycan	20	23	PF01048	0.615
MOD_GlcNHglycan	64	67	PF01048	0.429
MOD_GSK3_1	158	165	PF00069	0.210
MOD_GSK3_1	255	262	PF00069	0.294
MOD_GSK3_1	289	296	PF00069	0.270
MOD_N-GLC_1	107	112	PF02516	0.454
MOD_N-GLC_1	250	255	PF02516	0.485
MOD_N-GLC_2	194	196	PF02516	0.415
MOD_NEK2_1	129	134	PF00069	0.317
MOD_NEK2_1	17	22	PF00069	0.493
MOD_NEK2_1	222	227	PF00069	0.267
MOD_NEK2_1	289	294	PF00069	0.297
MOD_NEK2_1	62	67	PF00069	0.210
MOD_PKA_2	161	167	PF00069	0.215
MOD_PKA_2	277	283	PF00069	0.291
MOD_Plk_1	242	248	PF00069	0.266
MOD_Plk_1	250	256	PF00069	0.276
MOD_Plk_1	300	306	PF00069	0.470
MOD_Plk_2-3	300	306	PF00069	0.351
MOD_Plk_4	289	295	PF00069	0.256
MOD_Plk_4	9	15	PF00069	0.419
MOD_ProDKin_1	196	202	PF00069	0.206
MOD_ProDKin_1	255	261	PF00069	0.203
MOD_ProDKin_1	68	74	PF00069	0.303
TRG_DiLeu_BaLyEn_6	218	223	PF01217	0.215
TRG_ENDOCYTIC_2	209	212	PF00928	0.271
TRG_ENDOCYTIC_2	254	257	PF00928	0.235
TRG_NLS_MonoExtN_4	183	188	PF00514	0.159
TRG_Pf-PMV_PEXEL_1	233	237	PF00026	0.391
TRG_Pf-PMV_PEXEL_1	45	49	PF00026	0.433

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8Q7	Leptomonas seymouri	60%	88%
A0A0S4JAL3	Bodo saltans	37%	93%
A0A0S4JML7	Bodo saltans	30%	87%
A0A1X0P2K7	Trypanosomatidae	43%	96%
A0A3Q8IBF6	Leishmania donovani	39%	100%
A0A3Q8IFH4	Leishmania donovani	37%	100%
A0A3Q8IK90	Leishmania donovani	39%	91%
A0A3S7X3A6	Leishmania donovani	94%	100%
A0A422N7B0	Trypanosoma rangeli	44%	96%
A4H6V5	Leishmania braziliensis	39%	100%
A4HI96	Leishmania braziliensis	64%	100%
A4HJL3	Leishmania braziliensis	39%	100%
A4I5I0	Leishmania infantum	94%	100%
A4I736	Leishmania infantum	37%	100%
C9ZQH5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
C9ZQH6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
C9ZQW0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	97%
E9AGC7	Leishmania infantum	39%	100%
E9ANW7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E9B0S4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	82%	100%
E9B0S5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	100%
E9B231	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
F4JJL0	Arabidopsis thaliana	27%	100%
F4JJL3	Arabidopsis thaliana	26%	100%
P24021	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	26%	100%
Q4Q630	Leishmania major	37%	100%
Q4Q7F3	Leishmania major	86%	100%
Q4Q7F4	Leishmania major	86%	100%
Q4QGQ3	Leishmania major	39%	100%
Q66VY6	Leishmania major	38%	100%
Q8LDW6	Arabidopsis thaliana	25%	100%
Q9C9G4	Arabidopsis thaliana	26%	100%
Q9SXA6	Arabidopsis thaliana	25%	100%
V5B8Z9	Trypanosoma cruzi	42%	81%