E3 ubiquitin-protein ligase, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

E3 ubiquitin-protein ligase, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IEV4_LEIDO

TriTrypDb:

LdBPK_302180.1 , LdCL_300027300 , LDHU3_30.2950

Length:

526

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0000151	ubiquitin ligase complex	3	1
GO:0005737	cytoplasm	2	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

A0A3Q8IEV4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IEV4

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009893	positive regulation of metabolic process	4	1
GO:0009894	regulation of catabolic process	4	1
GO:0009896	positive regulation of catabolic process	5	1
GO:0009987	cellular process	1	1
GO:0010604	positive regulation of macromolecule metabolic process	5	1
GO:0016567	protein ubiquitination	7	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0030162	regulation of proteolysis	6	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031325	positive regulation of cellular metabolic process	5	1
GO:0031329	regulation of cellular catabolic process	5	1
GO:0031331	positive regulation of cellular catabolic process	6	1
GO:0032434	regulation of proteasomal ubiquitin-dependent protein catabolic process	7	1
GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process	8	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0042176	regulation of protein catabolic process	5	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0045732	positive regulation of protein catabolic process	6	1
GO:0045862	positive regulation of proteolysis	7	1
GO:0048518	positive regulation of biological process	3	1
GO:0048522	positive regulation of cellular process	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051173	positive regulation of nitrogen compound metabolic process	5	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0051247	positive regulation of protein metabolic process	6	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0061136	regulation of proteasomal protein catabolic process	6	1
GO:0065007	biological regulation	1	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901575	organic substance catabolic process	3	1
GO:1901800	positive regulation of proteasomal protein catabolic process	7	1
GO:1903050	regulation of proteolysis involved in protein catabolic process	7	1
GO:1903052	positive regulation of proteolysis involved in protein catabolic process	8	1
GO:2000058	regulation of ubiquitin-dependent protein catabolic process	6	1
GO:2000060	positive regulation of ubiquitin-dependent protein catabolic process	7	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0004842	ubiquitin-protein transferase activity	4	8
GO:0005488	binding	1	7
GO:0016740	transferase activity	2	8
GO:0019787	ubiquitin-like protein transferase activity	3	8
GO:0043167	ion binding	2	7
GO:0043169	cation binding	3	7
GO:0046872	metal ion binding	4	7
GO:0140096	catalytic activity, acting on a protein	2	8
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0031624	ubiquitin conjugating enzyme binding	5	1
GO:0044390	ubiquitin-like protein conjugating enzyme binding	4	1
GO:0061630	ubiquitin protein ligase activity	5	1
GO:0061659	ubiquitin-like protein ligase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	363	367	PF00656	0.542
CLV_NRD_NRD_1	219	221	PF00675	0.364
CLV_NRD_NRD_1	369	371	PF00675	0.500
CLV_NRD_NRD_1	410	412	PF00675	0.547
CLV_NRD_NRD_1	89	91	PF00675	0.498
CLV_PCSK_KEX2_1	369	371	PF00082	0.563
CLV_PCSK_KEX2_1	410	412	PF00082	0.583
CLV_PCSK_KEX2_1	89	91	PF00082	0.456
CLV_PCSK_SKI1_1	212	216	PF00082	0.471
CLV_PCSK_SKI1_1	221	225	PF00082	0.304
CLV_PCSK_SKI1_1	259	263	PF00082	0.361
CLV_PCSK_SKI1_1	277	281	PF00082	0.476
CLV_PCSK_SKI1_1	369	373	PF00082	0.478
CLV_PCSK_SKI1_1	404	408	PF00082	0.539
CLV_PCSK_SKI1_1	454	458	PF00082	0.385
CLV_PCSK_SKI1_1	497	501	PF00082	0.534
DOC_CDC14_PxL_1	247	255	PF14671	0.417
DOC_MAPK_DCC_7	426	435	PF00069	0.499
DOC_MAPK_gen_1	89	99	PF00069	0.482
DOC_MAPK_MEF2A_6	426	435	PF00069	0.601
DOC_MAPK_RevD_3	74	90	PF00069	0.431
DOC_PP1_SILK_1	212	217	PF00149	0.355
DOC_USP7_MATH_1	39	43	PF00917	0.604
DOC_USP7_MATH_1	501	505	PF00917	0.429
DOC_USP7_MATH_1	64	68	PF00917	0.526
DOC_USP7_UBL2_3	212	216	PF12436	0.408
DOC_USP7_UBL2_3	313	317	PF12436	0.352
LIG_14-3-3_CanoR_1	270	274	PF00244	0.368
LIG_14-3-3_CanoR_1	277	284	PF00244	0.360
LIG_14-3-3_CanoR_1	285	291	PF00244	0.232
LIG_14-3-3_CanoR_1	369	374	PF00244	0.496
LIG_14-3-3_CanoR_1	377	381	PF00244	0.383
LIG_14-3-3_CanoR_1	445	451	PF00244	0.498
LIG_Actin_WH2_2	254	272	PF00022	0.417
LIG_APCC_ABBAyCdc20_2	377	383	PF00400	0.437
LIG_BRCT_BRCA1_1	367	371	PF00533	0.488
LIG_deltaCOP1_diTrp_1	331	340	PF00928	0.360
LIG_FHA_1	105	111	PF00498	0.436
LIG_FHA_1	222	228	PF00498	0.486
LIG_FHA_1	377	383	PF00498	0.437
LIG_FHA_1	437	443	PF00498	0.515
LIG_FHA_1	445	451	PF00498	0.438
LIG_FHA_1	54	60	PF00498	0.454
LIG_FHA_1	83	89	PF00498	0.393
LIG_FHA_2	398	404	PF00498	0.573
LIG_FHA_2	450	456	PF00498	0.474
LIG_FHA_2	506	512	PF00498	0.526
LIG_LIR_Gen_1	331	339	PF02991	0.358
LIG_LIR_Gen_1	460	467	PF02991	0.461
LIG_LIR_Nem_3	331	337	PF02991	0.358
LIG_LIR_Nem_3	372	378	PF02991	0.496
LIG_PTB_Apo_2	261	268	PF02174	0.417
LIG_PTB_Phospho_1	261	267	PF10480	0.417
LIG_SH2_CRK	247	251	PF00017	0.417
LIG_SH2_NCK_1	247	251	PF00017	0.417
LIG_SH2_SRC	10	13	PF00017	0.683
LIG_SH2_SRC	247	250	PF00017	0.417
LIG_SH2_SRC	273	276	PF00017	0.417
LIG_SH2_STAP1	14	18	PF00017	0.634
LIG_SH2_STAP1	378	382	PF00017	0.433
LIG_SH2_STAT3	144	147	PF00017	0.414
LIG_SH2_STAT3	28	31	PF00017	0.784
LIG_SH2_STAT5	102	105	PF00017	0.434
LIG_SH2_STAT5	158	161	PF00017	0.382
LIG_SH2_STAT5	222	225	PF00017	0.309
LIG_SH2_STAT5	267	270	PF00017	0.417
LIG_SH2_STAT5	290	293	PF00017	0.487
LIG_SH2_STAT5	300	303	PF00017	0.514
LIG_SH2_STAT5	378	381	PF00017	0.430
LIG_SH2_STAT5	461	464	PF00017	0.332
LIG_SH2_STAT5	466	469	PF00017	0.411
LIG_SH3_3	246	252	PF00018	0.364
LIG_SH3_3	272	278	PF00018	0.300
LIG_TRAF2_1	360	363	PF00917	0.654
LIG_TYR_ITIM	245	250	PF00017	0.417
LIG_UBA3_1	214	221	PF00899	0.417
LIG_ULM_U2AF65_1	89	94	PF00076	0.456
LIG_WRC_WIRS_1	223	228	PF05994	0.337
LIG_WRC_WIRS_1	506	511	PF05994	0.530
MOD_CK1_1	289	295	PF00069	0.534
MOD_CK1_1	358	364	PF00069	0.558
MOD_CK1_1	444	450	PF00069	0.478
MOD_CK1_1	82	88	PF00069	0.399
MOD_CK2_1	117	123	PF00069	0.515
MOD_CK2_1	222	228	PF00069	0.354
MOD_CK2_1	397	403	PF00069	0.568
MOD_Cter_Amidation	1	4	PF01082	0.528
MOD_GlcNHglycan	316	320	PF01048	0.360
MOD_GlcNHglycan	343	346	PF01048	0.532
MOD_GlcNHglycan	357	360	PF01048	0.609
MOD_GlcNHglycan	383	386	PF01048	0.456
MOD_GlcNHglycan	39	42	PF01048	0.692
MOD_GlcNHglycan	421	424	PF01048	0.721
MOD_GlcNHglycan	491	494	PF01048	0.449
MOD_GlcNHglycan	66	69	PF01048	0.509
MOD_GlcNHglycan	81	84	PF01048	0.378
MOD_GSK3_1	104	111	PF00069	0.473
MOD_GSK3_1	113	120	PF00069	0.412
MOD_GSK3_1	222	229	PF00069	0.344
MOD_GSK3_1	311	318	PF00069	0.417
MOD_GSK3_1	33	40	PF00069	0.664
MOD_GSK3_1	365	372	PF00069	0.579
MOD_GSK3_1	433	440	PF00069	0.524
MOD_GSK3_1	485	492	PF00069	0.387
MOD_GSK3_1	497	504	PF00069	0.470
MOD_GSK3_1	53	60	PF00069	0.476
MOD_GSK3_1	74	81	PF00069	0.468
MOD_LATS_1	219	225	PF00433	0.386
MOD_N-GLC_1	104	109	PF02516	0.438
MOD_N-GLC_1	263	268	PF02516	0.354
MOD_N-GLC_1	469	474	PF02516	0.486
MOD_N-GLC_1	74	79	PF02516	0.487
MOD_NEK2_1	103	108	PF00069	0.453
MOD_NEK2_1	176	181	PF00069	0.405
MOD_NEK2_1	226	231	PF00069	0.363
MOD_NEK2_1	302	307	PF00069	0.360
MOD_NEK2_1	391	396	PF00069	0.577
MOD_NEK2_1	457	462	PF00069	0.453
MOD_NEK2_1	491	496	PF00069	0.510
MOD_NEK2_1	499	504	PF00069	0.446
MOD_NEK2_1	79	84	PF00069	0.414
MOD_OFUCOSY	321	326	PF10250	0.300
MOD_PIKK_1	501	507	PF00454	0.596
MOD_PK_1	210	216	PF00069	0.370
MOD_PKA_1	369	375	PF00069	0.511
MOD_PKA_2	269	275	PF00069	0.478
MOD_PKA_2	369	375	PF00069	0.471
MOD_PKA_2	376	382	PF00069	0.463
MOD_PKA_2	444	450	PF00069	0.529
MOD_PKA_2	91	97	PF00069	0.477
MOD_Plk_1	104	110	PF00069	0.472
MOD_Plk_1	117	123	PF00069	0.381
MOD_Plk_1	263	269	PF00069	0.401
MOD_Plk_1	53	59	PF00069	0.469
MOD_Plk_1	74	80	PF00069	0.476
MOD_Plk_2-3	117	123	PF00069	0.515
MOD_Plk_2-3	197	203	PF00069	0.376
MOD_Plk_2-3	53	59	PF00069	0.532
MOD_Plk_4	190	196	PF00069	0.391
MOD_Plk_4	210	216	PF00069	0.368
MOD_Plk_4	222	228	PF00069	0.350
MOD_Plk_4	33	39	PF00069	0.677
MOD_Plk_4	446	452	PF00069	0.412
MOD_Plk_4	457	463	PF00069	0.376
MOD_Plk_4	491	497	PF00069	0.519
MOD_Plk_4	505	511	PF00069	0.339
MOD_Plk_4	82	88	PF00069	0.378
MOD_SUMO_for_1	425	428	PF00179	0.597
MOD_SUMO_rev_2	117	127	PF00179	0.515
MOD_SUMO_rev_2	383	392	PF00179	0.478
MOD_SUMO_rev_2	40	50	PF00179	0.574
TRG_DiLeu_BaLyEn_6	401	406	PF01217	0.531
TRG_ENDOCYTIC_2	158	161	PF00928	0.440
TRG_ENDOCYTIC_2	247	250	PF00928	0.417
TRG_ENDOCYTIC_2	461	464	PF00928	0.332
TRG_ER_diArg_1	369	371	PF00400	0.461
TRG_ER_diArg_1	410	412	PF00400	0.547
TRG_ER_diArg_1	88	90	PF00400	0.447

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8P3	Leptomonas seymouri	49%	100%
A0A0S4JJP0	Bodo saltans	27%	100%
A0A1X0P1P5	Trypanosomatidae	30%	100%
A0A3R7JXL5	Trypanosoma rangeli	28%	100%
A2VEA3	Bos taurus	27%	95%
A4HIE9	Leishmania braziliensis	80%	100%
A4I5P6	Leishmania infantum	99%	100%
B1H1E4	Xenopus tropicalis	28%	99%
E9B0Z0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
O01965	Caenorhabditis elegans	26%	100%
O76924	Drosophila melanogaster	24%	100%
P0C8K8	Arabidopsis thaliana	27%	95%
P36113	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	95%
Q22431	Caenorhabditis elegans	27%	100%
Q32NS4	Xenopus laevis	28%	99%
Q6NW85	Danio rerio	28%	99%
Q6PFJ9	Danio rerio	28%	100%
Q6T486	Dictyostelium discoideum	27%	100%
Q84RQ8	Arabidopsis thaliana	25%	100%
Q84RR0	Arabidopsis thaliana	30%	94%
Q84RR2	Arabidopsis thaliana	25%	89%
Q8L829	Arabidopsis thaliana	29%	95%
Q8W468	Arabidopsis thaliana	28%	93%
Q94981	Drosophila melanogaster	26%	100%
Q949V6	Arabidopsis thaliana	25%	88%
Q9LVW9	Arabidopsis thaliana	26%	99%
Q9LVX0	Arabidopsis thaliana	26%	98%
Q9NE54	Leishmania major	94%	100%
Q9P3U4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%
Q9SKC3	Arabidopsis thaliana	28%	97%
Q9Y4X5	Homo sapiens	27%	94%
Q9Z1K5	Mus musculus	27%	95%
V5AQL7	Trypanosoma cruzi	30%	100%