H(+)-exporting diphosphatase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Pyrophosphate-energized vacuolar membrane proton pump 1, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IEU2_LEIDO

TriTrypDb:

LdBPK_311240.1 , LdCL_310019100 , LDHU3_31.2020

Length:

801

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 7

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 12, no: 0
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	13
GO:0110165	cellular anatomical entity	1	13
GO:0000139	Golgi membrane	5	1
GO:0000331	contractile vacuole	6	1
GO:0005773	vacuole	5	1
GO:0020022	acidocalcisome	5	1
GO:0031090	organelle membrane	3	1
GO:0031410	cytoplasmic vesicle	6	1
GO:0031982	vesicle	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097708	intracellular vesicle	5	1
GO:0098588	bounding membrane of organelle	4	1

Expansion

Sequence features

A0A3Q8IEU2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IEU2

Function

Biological processes
Term	Name	Level	Count
GO:0006873	intracellular monoatomic ion homeostasis	4	1
GO:0006874	intracellular calcium ion homeostasis	7	1
GO:0006875	obsolete intracellular metal ion homeostasis	6	1
GO:0006885	regulation of pH	8	1
GO:0009987	cellular process	1	1
GO:0019725	cellular homeostasis	2	1
GO:0030003	intracellular monoatomic cation homeostasis	5	1
GO:0030004	obsolete cellular monovalent inorganic cation homeostasis	6	1
GO:0030641	regulation of cellular pH	7	1
GO:0042592	homeostatic process	3	1
GO:0048878	chemical homeostasis	4	1
GO:0050801	monoatomic ion homeostasis	5	1
GO:0051452	intracellular pH reduction	9	1
GO:0051453	regulation of intracellular pH	8	1
GO:0055065	obsolete metal ion homeostasis	7	1
GO:0055067	obsolete monovalent inorganic cation homeostasis	7	1
GO:0055074	calcium ion homeostasis	8	1
GO:0055080	monoatomic cation homeostasis	6	1
GO:0055082	intracellular chemical homeostasis	3	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0072503	obsolete cellular divalent inorganic cation homeostasis	6	1
GO:0072507	obsolete divalent inorganic cation homeostasis	7	1
GO:0098771	inorganic ion homeostasis	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0004427	inorganic diphosphate phosphatase activity	6	13
GO:0005215	transporter activity	1	13
GO:0008324	monoatomic cation transmembrane transporter activity	4	13
GO:0009678	pyrophosphate hydrolysis-driven proton transmembrane transporter activity	5	13
GO:0015075	monoatomic ion transmembrane transporter activity	3	13
GO:0015078	proton transmembrane transporter activity	5	13
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	13
GO:0015399	primary active transmembrane transporter activity	4	13
GO:0016462	pyrophosphatase activity	5	13
GO:0016787	hydrolase activity	2	13
GO:0016817	hydrolase activity, acting on acid anhydrides	3	13
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	13
GO:0022804	active transmembrane transporter activity	3	13
GO:0022853	active monoatomic ion transmembrane transporter activity	4	13
GO:0022857	transmembrane transporter activity	2	13
GO:0022890	inorganic cation transmembrane transporter activity	4	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	560	564	PF00656	0.540
CLV_NRD_NRD_1	645	647	PF00675	0.340
CLV_NRD_NRD_1	656	658	PF00675	0.340
CLV_PCSK_KEX2_1	402	404	PF00082	0.451
CLV_PCSK_KEX2_1	645	647	PF00082	0.340
CLV_PCSK_PC1ET2_1	402	404	PF00082	0.449
CLV_PCSK_SKI1_1	403	407	PF00082	0.439
CLV_PCSK_SKI1_1	559	563	PF00082	0.340
CLV_PCSK_SKI1_1	671	675	PF00082	0.391
CLV_Separin_Metazoa	105	109	PF03568	0.540
DEG_MDM2_SWIB_1	76	84	PF02201	0.434
DEG_SCF_FBW7_1	27	34	PF00400	0.512
DOC_CKS1_1	28	33	PF01111	0.586
DOC_CYCLIN_RxL_1	400	409	PF00134	0.574
DOC_CYCLIN_yCln2_LP_2	6	12	PF00134	0.556
DOC_MAPK_DCC_7	4	12	PF00069	0.561
DOC_MAPK_DCC_7	598	606	PF00069	0.374
DOC_MAPK_HePTP_8	772	784	PF00069	0.392
DOC_MAPK_MEF2A_6	4	12	PF00069	0.673
DOC_MAPK_MEF2A_6	598	606	PF00069	0.374
DOC_MAPK_MEF2A_6	610	617	PF00069	0.374
DOC_MAPK_MEF2A_6	763	772	PF00069	0.540
DOC_MAPK_MEF2A_6	775	784	PF00069	0.392
DOC_MAPK_MEF2A_6	791	799	PF00069	0.398
DOC_PP1_RVXF_1	216	222	PF00149	0.646
DOC_PP2B_LxvP_1	6	9	PF13499	0.560
DOC_PP4_FxxP_1	648	651	PF00568	0.540
DOC_PP4_MxPP_1	677	680	PF00568	0.487
DOC_USP7_MATH_1	179	183	PF00917	0.421
DOC_USP7_MATH_1	351	355	PF00917	0.340
DOC_USP7_MATH_1	386	390	PF00917	0.550
DOC_USP7_MATH_1	549	553	PF00917	0.631
DOC_USP7_MATH_1	55	59	PF00917	0.477
DOC_USP7_MATH_1	580	584	PF00917	0.392
DOC_USP7_MATH_1	594	598	PF00917	0.340
DOC_USP7_MATH_1	707	711	PF00917	0.392
DOC_USP7_UBL2_3	740	744	PF12436	0.581
DOC_WW_Pin1_4	150	155	PF00397	0.348
DOC_WW_Pin1_4	160	165	PF00397	0.312
DOC_WW_Pin1_4	27	32	PF00397	0.635
DOC_WW_Pin1_4	684	689	PF00397	0.421
DOC_WW_Pin1_4	743	748	PF00397	0.540
DOC_WW_Pin1_4	87	92	PF00397	0.608
LIG_14-3-3_CanoR_1	116	124	PF00244	0.540
LIG_14-3-3_CanoR_1	226	232	PF00244	0.540
LIG_14-3-3_CanoR_1	54	64	PF00244	0.475
LIG_Actin_WH2_2	82	100	PF00022	0.661
LIG_BIR_II_1	1	5	PF00653	0.732
LIG_BIR_III_4	536	540	PF00653	0.392
LIG_BIR_III_4	566	570	PF00653	0.540
LIG_BRCT_BRCA1_1	333	337	PF00533	0.392
LIG_BRCT_BRCA1_1	780	784	PF00533	0.392
LIG_eIF4E_1	698	704	PF01652	0.313
LIG_FHA_1	101	107	PF00498	0.607
LIG_FHA_1	120	126	PF00498	0.540
LIG_FHA_1	244	250	PF00498	0.369
LIG_FHA_1	411	417	PF00498	0.405
LIG_FHA_1	425	431	PF00498	0.350
LIG_FHA_1	480	486	PF00498	0.540
LIG_FHA_1	503	509	PF00498	0.443
LIG_FHA_1	526	532	PF00498	0.392
LIG_FHA_1	560	566	PF00498	0.540
LIG_FHA_1	570	576	PF00498	0.540
LIG_FHA_1	59	65	PF00498	0.333
LIG_FHA_2	258	264	PF00498	0.435
LIG_FHA_2	32	38	PF00498	0.616
LIG_FHA_2	755	761	PF00498	0.540
LIG_LIR_Gen_1	128	139	PF02991	0.514
LIG_LIR_Gen_1	171	180	PF02991	0.421
LIG_LIR_Gen_1	263	273	PF02991	0.350
LIG_LIR_Gen_1	358	369	PF02991	0.340
LIG_LIR_Gen_1	85	91	PF02991	0.638
LIG_LIR_Nem_3	128	134	PF02991	0.390
LIG_LIR_Nem_3	137	142	PF02991	0.385
LIG_LIR_Nem_3	171	176	PF02991	0.421
LIG_LIR_Nem_3	263	268	PF02991	0.350
LIG_LIR_Nem_3	334	340	PF02991	0.392
LIG_LIR_Nem_3	363	369	PF02991	0.377
LIG_LIR_Nem_3	435	441	PF02991	0.361
LIG_LIR_Nem_3	463	469	PF02991	0.532
LIG_LIR_Nem_3	504	510	PF02991	0.429
LIG_LIR_Nem_3	536	541	PF02991	0.392
LIG_LIR_Nem_3	75	79	PF02991	0.393
LIG_LIR_Nem_3	781	787	PF02991	0.392
LIG_LIR_Nem_3	85	89	PF02991	0.595
LIG_LYPXL_S_1	365	369	PF13949	0.540
LIG_LYPXL_yS_3	366	369	PF13949	0.340
LIG_Pex14_2	135	139	PF04695	0.392
LIG_Pex14_2	217	221	PF04695	0.526
LIG_Pex14_2	76	80	PF04695	0.405
LIG_Pex14_2	784	788	PF04695	0.439
LIG_SH2_CRK	114	118	PF00017	0.610
LIG_SH2_CRK	131	135	PF00017	0.265
LIG_SH2_CRK	466	470	PF00017	0.540
LIG_SH2_CRK	538	542	PF00017	0.540
LIG_SH2_GRB2like	195	198	PF00017	0.540
LIG_SH2_NCK_1	114	118	PF00017	0.631
LIG_SH2_NCK_1	265	269	PF00017	0.361
LIG_SH2_NCK_1	289	293	PF00017	0.550
LIG_SH2_NCK_1	698	702	PF00017	0.340
LIG_SH2_STAP1	265	269	PF00017	0.361
LIG_SH2_STAP1	289	293	PF00017	0.550
LIG_SH2_STAP1	456	460	PF00017	0.392
LIG_SH2_STAP1	461	465	PF00017	0.540
LIG_SH2_STAP1	662	666	PF00017	0.550
LIG_SH2_STAP1	82	86	PF00017	0.641
LIG_SH2_STAT3	731	734	PF00017	0.607
LIG_SH2_STAT5	111	114	PF00017	0.537
LIG_SH2_STAT5	195	198	PF00017	0.538
LIG_SH2_STAT5	253	256	PF00017	0.340
LIG_SH2_STAT5	289	292	PF00017	0.550
LIG_SH2_STAT5	456	459	PF00017	0.413
LIG_SH2_STAT5	461	464	PF00017	0.496
LIG_SH2_STAT5	492	495	PF00017	0.392
LIG_SH2_STAT5	507	510	PF00017	0.392
LIG_SH2_STAT5	595	598	PF00017	0.361
LIG_SH2_STAT5	698	701	PF00017	0.333
LIG_SH2_STAT5	731	734	PF00017	0.532
LIG_SH3_3	158	164	PF00018	0.400
LIG_SH3_3	21	27	PF00018	0.599
LIG_SH3_3	309	315	PF00018	0.631
LIG_SH3_3	419	425	PF00018	0.431
LIG_SH3_3	492	498	PF00018	0.392
LIG_SH3_3	596	602	PF00018	0.374
LIG_SH3_3	755	761	PF00018	0.540
LIG_SH3_3	780	786	PF00018	0.392
LIG_Sin3_3	11	18	PF02671	0.648
LIG_SUMO_SIM_anti_2	413	418	PF11976	0.438
LIG_SUMO_SIM_anti_2	63	69	PF11976	0.461
LIG_SUMO_SIM_par_1	366	371	PF11976	0.340
LIG_SUMO_SIM_par_1	682	687	PF11976	0.434
LIG_TRAF2_1	153	156	PF00917	0.423
LIG_TRAF2_1	468	471	PF00917	0.523
LIG_TYR_ITIM	112	117	PF00017	0.625
LIG_TYR_ITIM	490	495	PF00017	0.540
LIG_UBA3_1	240	248	PF00899	0.369
LIG_Vh1_VBS_1	369	387	PF01044	0.424
LIG_WRC_WIRS_1	337	342	PF05994	0.392
MOD_CDK_SPxK_1	87	93	PF00069	0.615
MOD_CK1_1	360	366	PF00069	0.404
MOD_CK1_1	527	533	PF00069	0.393
MOD_CK1_1	58	64	PF00069	0.449
MOD_CK1_1	626	632	PF00069	0.392
MOD_CK2_1	150	156	PF00069	0.423
MOD_CK2_1	253	259	PF00069	0.430
MOD_CK2_1	336	342	PF00069	0.392
MOD_Cter_Amidation	655	658	PF01082	0.431
MOD_GlcNHglycan	136	139	PF01048	0.546
MOD_GlcNHglycan	205	208	PF01048	0.390
MOD_GlcNHglycan	214	217	PF01048	0.268
MOD_GlcNHglycan	255	258	PF01048	0.588
MOD_GlcNHglycan	275	278	PF01048	0.330
MOD_GlcNHglycan	353	356	PF01048	0.551
MOD_GlcNHglycan	388	391	PF01048	0.348
MOD_GlcNHglycan	449	452	PF01048	0.385
MOD_GlcNHglycan	57	60	PF01048	0.623
MOD_GlcNHglycan	582	585	PF01048	0.392
MOD_GlcNHglycan	625	628	PF01048	0.400
MOD_GlcNHglycan	709	712	PF01048	0.392
MOD_GlcNHglycan	716	719	PF01048	0.392
MOD_GlcNHglycan	764	768	PF01048	0.340
MOD_GSK3_1	115	122	PF00069	0.550
MOD_GSK3_1	134	141	PF00069	0.487
MOD_GSK3_1	193	200	PF00069	0.560
MOD_GSK3_1	253	260	PF00069	0.420
MOD_GSK3_1	27	34	PF00069	0.604
MOD_GSK3_1	351	358	PF00069	0.340
MOD_GSK3_1	380	387	PF00069	0.519
MOD_GSK3_1	406	413	PF00069	0.391
MOD_GSK3_1	455	462	PF00069	0.392
MOD_GSK3_1	475	482	PF00069	0.534
MOD_GSK3_1	502	509	PF00069	0.394
MOD_GSK3_1	527	534	PF00069	0.405
MOD_GSK3_1	714	721	PF00069	0.405
MOD_N-GLC_1	166	171	PF02516	0.646
MOD_N-GLC_1	569	574	PF02516	0.340
MOD_NEK2_1	166	171	PF00069	0.431
MOD_NEK2_1	203	208	PF00069	0.631
MOD_NEK2_1	233	238	PF00069	0.398
MOD_NEK2_1	243	248	PF00069	0.380
MOD_NEK2_1	273	278	PF00069	0.384
MOD_NEK2_1	336	341	PF00069	0.392
MOD_NEK2_1	357	362	PF00069	0.370
MOD_NEK2_1	368	373	PF00069	0.338
MOD_NEK2_1	384	389	PF00069	0.392
MOD_NEK2_1	405	410	PF00069	0.386
MOD_NEK2_1	433	438	PF00069	0.428
MOD_NEK2_1	502	507	PF00069	0.386
MOD_NEK2_1	714	719	PF00069	0.487
MOD_NEK2_1	762	767	PF00069	0.540
MOD_NEK2_2	168	173	PF00069	0.360
MOD_PKA_2	115	121	PF00069	0.540
MOD_Plk_1	166	172	PF00069	0.446
MOD_Plk_1	433	439	PF00069	0.403
MOD_Plk_1	549	555	PF00069	0.608
MOD_Plk_4	138	144	PF00069	0.421
MOD_Plk_4	168	174	PF00069	0.351
MOD_Plk_4	360	366	PF00069	0.403
MOD_Plk_4	410	416	PF00069	0.382
MOD_Plk_4	491	497	PF00069	0.422
MOD_Plk_4	502	508	PF00069	0.402
MOD_Plk_4	60	66	PF00069	0.381
MOD_Plk_4	82	88	PF00069	0.628
MOD_ProDKin_1	150	156	PF00069	0.348
MOD_ProDKin_1	160	166	PF00069	0.312
MOD_ProDKin_1	27	33	PF00069	0.634
MOD_ProDKin_1	684	690	PF00069	0.421
MOD_ProDKin_1	743	749	PF00069	0.540
MOD_ProDKin_1	87	93	PF00069	0.615
MOD_SUMO_rev_2	127	132	PF00179	0.631
TRG_DiLeu_BaLyEn_6	364	369	PF01217	0.340
TRG_ENDOCYTIC_2	114	117	PF00928	0.594
TRG_ENDOCYTIC_2	129	132	PF00928	0.282
TRG_ENDOCYTIC_2	265	268	PF00928	0.361
TRG_ENDOCYTIC_2	366	369	PF00928	0.340
TRG_ENDOCYTIC_2	466	469	PF00928	0.540
TRG_ENDOCYTIC_2	492	495	PF00928	0.392
TRG_ENDOCYTIC_2	538	541	PF00928	0.540
TRG_ER_diArg_1	644	646	PF00400	0.540
TRG_Pf-PMV_PEXEL_1	559	563	PF00026	0.350

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5A1	Leptomonas seymouri	87%	100%
A0A0S4J2L1	Bodo saltans	76%	100%
A0A1X0NPP6	Trypanosomatidae	76%	95%
A0A422NED3	Trypanosoma rangeli	77%	99%
A4HJA5	Leishmania braziliensis	90%	100%
A4I6P8	Leishmania infantum	100%	100%
C9ZM75	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	73%	97%
C9ZWU7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	76%	97%
E9B1S0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
O68460	Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1)	41%	100%
P21616	Vigna radiata var. radiata	54%	100%
P31414	Arabidopsis thaliana	54%	100%
P60363	Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)	38%	100%
Q06572	Hordeum vulgare	55%	100%
Q2RIS7	Moorella thermoacetica (strain ATCC 39073 / JCM 9320)	48%	100%
Q2RLE0	Moorella thermoacetica (strain ATCC 39073 / JCM 9320)	41%	100%
Q3AFC6	Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)	44%	100%
Q4Q6E1	Leishmania major	97%	100%
Q56ZN6	Arabidopsis thaliana	39%	100%
Q72Q29	Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)	52%	100%
Q82EJ8	Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)	37%	100%
Q82TF3	Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)	41%	100%
Q898Q9	Clostridium tetani (strain Massachusetts / E88)	46%	100%
Q89K83	Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)	40%	100%
Q8A294	Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)	39%	100%
Q8F641	Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)	52%	100%
Q8G1E6	Brucella suis biovar 1 (strain 1330)	38%	100%
Q8KDT8	Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)	31%	100%
Q8KY01	Rhodopseudomonas palustris	40%	100%
Q8P5M6	Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)	41%	100%
Q8PH20	Xanthomonas axonopodis pv. citri (strain 306)	42%	100%
Q8PYZ7	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	43%	100%
Q8PYZ8	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	44%	100%
Q8RCX1	Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4)	40%	100%
Q8RHJ2	Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355)	44%	100%
Q8TJA8	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	44%	100%
Q8TJA9	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	44%	100%
Q8UG67	Agrobacterium fabrum (strain C58 / ATCC 33970)	37%	100%
Q8VNW3	Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)	36%	100%
Q8VRZ2	Brucella anthropi (strain ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 / LMG 3331 / NBRC 15819 / NCTC 12168 / Alc 37)	38%	100%
Q8VRZ3	Rhizobium meliloti (strain 1021)	39%	100%
Q8YGH4	Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)	38%	100%
Q8ZWI8	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	39%	100%
Q983A3	Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)	40%	100%
Q9A8J0	Caulobacter vibrioides (strain ATCC 19089 / CB15)	38%	100%
Q9FWR2	Arabidopsis thaliana	39%	100%
Q9S5X0	Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)	40%	100%
Q9X913	Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)	37%	100%
V5BFF6	Trypanosoma cruzi	78%	98%