Heat shock protein grpe

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

co-chaperone, GrpE, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IEN8_LEIDO

TriTrypDb:

LdBPK_300770.1 , LdCL_300012800 , LDHU3_30.0980

Length:

256

Annotations

LeishMANIAdb annotations

Homologous to other Eukaryotic mitochondrial proteins. Likely non-TM.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	2
GO:0110165	cellular anatomical entity	1	2
GO:0001405	PAM complex, Tim23 associated import motor	4	1
GO:0032991	protein-containing complex	1	1
GO:0098796	membrane protein complex	2	1
GO:0098798	mitochondrial protein-containing complex	2	1
GO:0098800	inner mitochondrial membrane protein complex	3	1

Expansion

Sequence features

A0A3Q8IEN8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IEN8

Function

Biological processes
Term	Name	Level	Count
GO:0006457	protein folding	2	7
GO:0009987	cellular process	1	7
GO:0006810	transport	3	1
GO:0006839	mitochondrial transport	4	1
GO:0006886	intracellular protein transport	4	1
GO:0008104	protein localization	4	1
GO:0015031	protein transport	4	1
GO:0030150	protein import into mitochondrial matrix	4	1
GO:0033036	macromolecule localization	2	1
GO:0033365	protein localization to organelle	5	1
GO:0044743	protein transmembrane import into intracellular organelle	4	1
GO:0045184	establishment of protein localization	3	1
GO:0046907	intracellular transport	3	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0055085	transmembrane transport	2	1
GO:0065002	intracellular protein transmembrane transport	4	1
GO:0070585	protein localization to mitochondrion	6	1
GO:0070727	cellular macromolecule localization	3	1
GO:0071702	organic substance transport	4	1
GO:0071705	nitrogen compound transport	4	1
GO:0071806	protein transmembrane transport	3	1
GO:0072594	establishment of protein localization to organelle	4	1
GO:0072655	establishment of protein localization to mitochondrion	5	1
GO:1990542	mitochondrial transmembrane transport	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	7
GO:0000774	adenyl-nucleotide exchange factor activity	3	7
GO:0005488	binding	1	7
GO:0005515	protein binding	2	7
GO:0017076	purine nucleotide binding	4	7
GO:0030554	adenyl nucleotide binding	5	7
GO:0036094	small molecule binding	2	7
GO:0042802	identical protein binding	3	7
GO:0042803	protein homodimerization activity	4	7
GO:0046983	protein dimerization activity	3	7
GO:0051087	protein-folding chaperone binding	3	7
GO:0060590	ATPase regulator activity	2	7
GO:0097159	organic cyclic compound binding	2	7
GO:0098772	molecular function regulator activity	1	7
GO:1901265	nucleoside phosphate binding	3	7
GO:1901363	heterocyclic compound binding	2	7
GO:0051082	unfolded protein binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	123	125	PF00675	0.243
CLV_NRD_NRD_1	246	248	PF00675	0.246
CLV_NRD_NRD_1	54	56	PF00675	0.499
CLV_NRD_NRD_1	71	73	PF00675	0.559
CLV_PCSK_KEX2_1	103	105	PF00082	0.254
CLV_PCSK_KEX2_1	193	195	PF00082	0.243
CLV_PCSK_KEX2_1	246	248	PF00082	0.285
CLV_PCSK_KEX2_1	54	56	PF00082	0.496
CLV_PCSK_PC1ET2_1	103	105	PF00082	0.254
CLV_PCSK_PC1ET2_1	193	195	PF00082	0.243
CLV_PCSK_SKI1_1	144	148	PF00082	0.228
CLV_PCSK_SKI1_1	159	163	PF00082	0.233
CLV_PCSK_SKI1_1	190	194	PF00082	0.300
CLV_PCSK_SKI1_1	215	219	PF00082	0.285
CLV_Separin_Metazoa	93	97	PF03568	0.454
DEG_APCC_DBOX_1	7	15	PF00400	0.395
DOC_CKS1_1	220	225	PF01111	0.428
DOC_MAPK_gen_1	246	252	PF00069	0.443
DOC_MAPK_gen_1	8	16	PF00069	0.393
DOC_MAPK_RevD_3	58	73	PF00069	0.497
DOC_PP4_FxxP_1	217	220	PF00568	0.485
DOC_USP7_MATH_1	116	120	PF00917	0.506
DOC_USP7_MATH_1	138	142	PF00917	0.485
DOC_USP7_UBL2_3	158	162	PF12436	0.401
DOC_WW_Pin1_4	219	224	PF00397	0.450
DOC_WW_Pin1_4	41	46	PF00397	0.438
LIG_14-3-3_CanoR_1	194	204	PF00244	0.485
LIG_Actin_WH2_2	212	228	PF00022	0.410
LIG_BIR_II_1	1	5	PF00653	0.417
LIG_FHA_1	182	188	PF00498	0.454
LIG_FHA_1	220	226	PF00498	0.443
LIG_FHA_1	78	84	PF00498	0.462
LIG_FHA_2	124	130	PF00498	0.422
LIG_FHA_2	205	211	PF00498	0.485
LIG_FHA_2	42	48	PF00498	0.407
LIG_LIR_Apic_2	214	220	PF02991	0.443
LIG_LIR_Nem_3	50	56	PF02991	0.475
LIG_Pex14_2	35	39	PF04695	0.433
LIG_SH2_CRK	53	57	PF00017	0.496
LIG_SH2_NCK_1	115	119	PF00017	0.485
LIG_SH2_STAT5	163	166	PF00017	0.428
LIG_SH3_3	217	223	PF00018	0.533
LIG_SUMO_SIM_anti_2	27	33	PF11976	0.399
LIG_SUMO_SIM_par_1	145	151	PF11976	0.467
LIG_SxIP_EBH_1	163	172	PF03271	0.454
LIG_TRAF2_1	126	129	PF00917	0.485
LIG_WRC_WIRS_1	139	144	PF05994	0.485
MOD_CDK_SPK_2	41	46	PF00069	0.438
MOD_CDK_SPxxK_3	219	226	PF00069	0.428
MOD_CK1_1	19	25	PF00069	0.530
MOD_CK1_1	221	227	PF00069	0.547
MOD_CK1_1	41	47	PF00069	0.432
MOD_CK2_1	123	129	PF00069	0.422
MOD_CK2_1	204	210	PF00069	0.518
MOD_CK2_1	221	227	PF00069	0.499
MOD_CK2_1	81	87	PF00069	0.590
MOD_GSK3_1	124	131	PF00069	0.552
MOD_GSK3_1	161	168	PF00069	0.456
MOD_GSK3_1	195	202	PF00069	0.446
MOD_GSK3_1	215	222	PF00069	0.470
MOD_GSK3_1	73	80	PF00069	0.512
MOD_N-GLC_1	166	171	PF02516	0.238
MOD_N-GLC_1	240	245	PF02516	0.243
MOD_NEK2_1	240	245	PF00069	0.473
MOD_NEK2_1	63	68	PF00069	0.597
MOD_NEK2_1	99	104	PF00069	0.516
MOD_PIKK_1	124	130	PF00454	0.516
MOD_PIKK_1	161	167	PF00454	0.454
MOD_PIKK_1	195	201	PF00454	0.485
MOD_PK_1	166	172	PF00069	0.464
MOD_PK_1	226	232	PF00069	0.428
MOD_PKA_1	124	130	PF00069	0.485
MOD_PKA_2	123	129	PF00069	0.422
MOD_PKA_2	16	22	PF00069	0.404
MOD_PKA_2	165	171	PF00069	0.563
MOD_Plk_1	166	172	PF00069	0.433
MOD_Plk_4	240	246	PF00069	0.443
MOD_ProDKin_1	219	225	PF00069	0.450
MOD_ProDKin_1	41	47	PF00069	0.434
MOD_SUMO_for_1	171	174	PF00179	0.454
MOD_SUMO_rev_2	209	217	PF00179	0.485
TRG_DiLeu_BaLyEn_6	59	64	PF01217	0.550
TRG_ENDOCYTIC_2	53	56	PF00928	0.503
TRG_ENDOCYTIC_2	92	95	PF00928	0.443
TRG_ER_diArg_1	245	247	PF00400	0.485
TRG_ER_diArg_1	53	55	PF00400	0.505
TRG_ER_diArg_1	8	11	PF00400	0.439
TRG_Pf-PMV_PEXEL_1	46	50	PF00026	0.443

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZM2	Leptomonas seymouri	48%	100%
A0A0N1ILR2	Leptomonas seymouri	29%	100%
A0A0S4JEA6	Bodo saltans	32%	100%
A0A1X0P2B4	Trypanosomatidae	30%	100%
A0A3Q8IJ81	Leishmania donovani	32%	100%
A0A422P4T2	Trypanosoma rangeli	25%	100%
A0KZB0	Shewanella sp. (strain ANA-3)	24%	100%
A0Q7F1	Francisella tularensis subsp. novicida (strain U112)	28%	100%
A1UUC9	Bartonella bacilliformis (strain ATCC 35685 / NCTC 12138 / KC583)	30%	100%
A3MZ85	Actinobacillus pleuropneumoniae serotype 5b (strain L20)	26%	100%
A4HI25	Leishmania braziliensis	32%	100%
A4HI26	Leishmania braziliensis	67%	98%
A4I5A2	Leishmania infantum	32%	100%
A4I5A3	Leishmania infantum	100%	100%
A4IX27	Francisella tularensis subsp. tularensis (strain WY96-3418)	27%	100%
A4SQ26	Aeromonas salmonicida (strain A449)	25%	100%
A5F369	Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)	25%	100%
A5VNA6	Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)	34%	100%
A6VMQ9	Actinobacillus succinogenes (strain ATCC 55618 / DSM 22257 / CCUG 43843 / 130Z)	25%	100%
A7H485	Campylobacter jejuni subsp. doylei (strain ATCC BAA-1458 / RM4099 / 269.97)	28%	100%
A7NCM8	Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA)	28%	100%
A8FLH1	Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)	27%	100%
A8FYL1	Shewanella sediminis (strain HAW-EB3)	27%	100%
A9M7B6	Brucella canis (strain ATCC 23365 / NCTC 10854)	34%	100%
B0BTB9	Actinobacillus pleuropneumoniae serotype 3 (strain JL03)	26%	100%
B0CJ30	Brucella suis (strain ATCC 23445 / NCTC 10510)	34%	100%
B1XRU2	Polynucleobacter necessarius subsp. necessarius (strain STIR1)	26%	100%
B2SGV9	Francisella tularensis subsp. mediasiatica (strain FSC147)	28%	100%
B3EPC6	Chlorobium phaeobacteroides (strain BS1)	25%	100%
B3H0M9	Actinobacillus pleuropneumoniae serotype 7 (strain AP76)	26%	100%
B4F059	Proteus mirabilis (strain HI4320)	27%	100%
B4S9D1	Prosthecochloris aestuarii (strain DSM 271 / SK 413)	27%	100%
C0QX60	Brachyspira hyodysenteriae (strain ATCC 49526 / WA1)	27%	100%
C3K276	Pseudomonas fluorescens (strain SBW25)	27%	100%
C3LTA4	Vibrio cholerae serotype O1 (strain M66-2)	25%	100%
C9ZQN6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9B0J8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9B0J9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
O30862	Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)	25%	100%
O69297	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	29%	100%
O88396	Mus musculus	28%	100%
P38523	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	100%
P48204	Francisella tularensis	28%	100%
Q0BLK5	Francisella tularensis subsp. holarctica (strain OSU18)	28%	100%
Q0HGL4	Shewanella sp. (strain MR-4)	24%	100%
Q0HSW5	Shewanella sp. (strain MR-7)	24%	100%
Q0P5N5	Bos taurus	28%	100%
Q14GW8	Francisella tularensis subsp. tularensis (strain FSC 198)	27%	100%
Q1MPH5	Lawsonia intracellularis (strain PHE/MN1-00)	26%	100%
Q21H35	Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)	27%	100%
Q2A329	Francisella tularensis subsp. holarctica (strain LVS)	28%	100%
Q2G6M5	Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199)	26%	100%
Q2GHU0	Ehrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas)	24%	100%
Q3IKR2	Pseudoalteromonas translucida (strain TAC 125)	25%	100%
Q3KIA1	Pseudomonas fluorescens (strain Pf0-1)	23%	100%
Q48E61	Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)	25%	100%
Q4Q7N3	Leishmania major	91%	100%
Q4Q7N4	Leishmania major	32%	100%
Q5NFG6	Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)	27%	100%
Q6BTP9	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	29%	100%
Q6CRQ1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	27%	100%
Q75C01	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	31%	100%
Q7MA34	Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W)	28%	100%
Q7UA77	Parasynechococcus marenigrum (strain WH8102)	26%	100%
Q7VMB7	Haemophilus ducreyi (strain 35000HP / ATCC 700724)	26%	100%
Q87WN9	Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)	25%	100%
Q8EGS0	Shewanella oneidensis (strain MR-1)	24%	100%
Q8G2Y6	Brucella suis biovar 1 (strain 1330)	34%	100%
Q8YEV0	Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)	34%	100%
Q9P5U4	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	30%	100%