GTPase activator protein, putative

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GTPase activator protein, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IEG1_LEIDO

TriTrypDb:

LdBPK_291670.1 , LdCL_290022300 , LDHU3_29.2420

Length:

529

Annotations

LeishMANIAdb annotations

Related to animal TBC proteins. Likely cytosolic.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A0A3Q8IEG1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IEG1

Function

Biological processes
Term	Name	Level	Count
GO:0043085	positive regulation of catalytic activity	4	1
GO:0043087	regulation of GTPase activity	5	1
GO:0043547	positive regulation of GTPase activity	6	1
GO:0044093	positive regulation of molecular function	3	1
GO:0050790	regulation of catalytic activity	3	1
GO:0051336	regulation of hydrolase activity	4	1
GO:0051345	positive regulation of hydrolase activity	5	1
GO:0065007	biological regulation	1	1
GO:0065009	regulation of molecular function	2	1
GO:0090630	activation of GTPase activity	7	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	5
GO:0003924	GTPase activity	7	5
GO:0003925	G protein activity	2	5
GO:0016462	pyrophosphatase activity	5	5
GO:0016787	hydrolase activity	2	5
GO:0016817	hydrolase activity, acting on acid anhydrides	3	5
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	5
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	5
GO:0098772	molecular function regulator activity	1	5
GO:0005096	GTPase activator activity	4	1
GO:0008047	enzyme activator activity	3	1
GO:0030234	enzyme regulator activity	2	1
GO:0030695	GTPase regulator activity	4	1
GO:0060589	nucleoside-triphosphatase regulator activity	3	1
GO:0140677	molecular function activator activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	7	11	PF00656	0.651
CLV_NRD_NRD_1	188	190	PF00675	0.427
CLV_NRD_NRD_1	35	37	PF00675	0.511
CLV_NRD_NRD_1	455	457	PF00675	0.377
CLV_NRD_NRD_1	485	487	PF00675	0.308
CLV_NRD_NRD_1	509	511	PF00675	0.406
CLV_NRD_NRD_1	92	94	PF00675	0.462
CLV_PCSK_KEX2_1	190	192	PF00082	0.376
CLV_PCSK_KEX2_1	35	37	PF00082	0.516
CLV_PCSK_KEX2_1	455	457	PF00082	0.328
CLV_PCSK_KEX2_1	485	487	PF00082	0.307
CLV_PCSK_KEX2_1	509	511	PF00082	0.409
CLV_PCSK_KEX2_1	92	94	PF00082	0.462
CLV_PCSK_PC1ET2_1	190	192	PF00082	0.335
DEG_SCF_FBW7_2	73	79	PF00400	0.642
DOC_CKS1_1	142	147	PF01111	0.681
DOC_CKS1_1	165	170	PF01111	0.529
DOC_CKS1_1	73	78	PF01111	0.649
DOC_CYCLIN_yClb3_PxF_3	278	286	PF00134	0.434
DOC_MAPK_gen_1	194	204	PF00069	0.549
DOC_MAPK_gen_1	375	383	PF00069	0.322
DOC_MAPK_gen_1	485	493	PF00069	0.538
DOC_MAPK_MEF2A_6	230	237	PF00069	0.422
DOC_MAPK_MEF2A_6	329	337	PF00069	0.288
DOC_MAPK_MEF2A_6	421	430	PF00069	0.488
DOC_MAPK_NFAT4_5	230	238	PF00069	0.422
DOC_PP1_RVXF_1	160	166	PF00149	0.525
DOC_PP1_RVXF_1	228	235	PF00149	0.449
DOC_PP4_FxxP_1	132	135	PF00568	0.594
DOC_PP4_FxxP_1	165	168	PF00568	0.577
DOC_USP7_MATH_1	116	120	PF00917	0.648
DOC_USP7_MATH_1	126	130	PF00917	0.711
DOC_USP7_MATH_1	147	151	PF00917	0.643
DOC_USP7_MATH_1	166	170	PF00917	0.439
DOC_USP7_MATH_1	522	526	PF00917	0.613
DOC_USP7_MATH_1	55	59	PF00917	0.693
DOC_USP7_MATH_1	61	65	PF00917	0.646
DOC_USP7_MATH_1	81	85	PF00917	0.596
DOC_USP7_MATH_1	98	102	PF00917	0.699
DOC_USP7_UBL2_3	190	194	PF12436	0.562
DOC_WW_Pin1_4	130	135	PF00397	0.718
DOC_WW_Pin1_4	141	146	PF00397	0.603
DOC_WW_Pin1_4	164	169	PF00397	0.554
DOC_WW_Pin1_4	206	211	PF00397	0.544
DOC_WW_Pin1_4	412	417	PF00397	0.513
DOC_WW_Pin1_4	419	424	PF00397	0.462
DOC_WW_Pin1_4	474	479	PF00397	0.493
DOC_WW_Pin1_4	72	77	PF00397	0.746
LIG_14-3-3_CanoR_1	200	205	PF00244	0.525
LIG_14-3-3_CanoR_1	455	462	PF00244	0.581
LIG_14-3-3_CanoR_1	469	475	PF00244	0.449
LIG_14-3-3_CanoR_1	56	60	PF00244	0.590
LIG_Actin_WH2_2	215	232	PF00022	0.474
LIG_APCC_ABBA_1	335	340	PF00400	0.226
LIG_BRCT_BRCA1_1	128	132	PF00533	0.599
LIG_BRCT_BRCA1_1	311	315	PF00533	0.348
LIG_Clathr_ClatBox_1	321	325	PF01394	0.255
LIG_DCNL_PONY_1	1	4	PF03556	0.591
LIG_eIF4E_1	249	255	PF01652	0.488
LIG_eIF4E_1	462	468	PF01652	0.496
LIG_FHA_1	292	298	PF00498	0.492
LIG_FHA_1	328	334	PF00498	0.229
LIG_FHA_1	456	462	PF00498	0.494
LIG_FHA_2	165	171	PF00498	0.532
LIG_FHA_2	181	187	PF00498	0.470
LIG_FHA_2	5	11	PF00498	0.648
LIG_GBD_Chelix_1	316	324	PF00786	0.385
LIG_LIR_Apic_2	129	135	PF02991	0.596
LIG_LIR_Gen_1	156	165	PF02991	0.657
LIG_LIR_Gen_1	312	321	PF02991	0.255
LIG_LIR_Gen_1	330	338	PF02991	0.222
LIG_LIR_Gen_1	393	402	PF02991	0.332
LIG_LIR_Nem_3	156	160	PF02991	0.557
LIG_LIR_Nem_3	192	198	PF02991	0.481
LIG_LIR_Nem_3	312	316	PF02991	0.264
LIG_LIR_Nem_3	328	334	PF02991	0.304
LIG_LIR_Nem_3	393	398	PF02991	0.332
LIG_PCNA_yPIPBox_3	358	371	PF02747	0.234
LIG_Pex14_1	408	412	PF04695	0.514
LIG_Pex14_2	315	319	PF04695	0.348
LIG_Pex14_2	395	399	PF04695	0.313
LIG_PTB_Apo_2	27	34	PF02174	0.551
LIG_PTB_Apo_2	341	348	PF02174	0.244
LIG_PTB_Phospho_1	341	347	PF10480	0.244
LIG_SH2_NCK_1	462	466	PF00017	0.500
LIG_SH2_STAP1	462	466	PF00017	0.500
LIG_SH2_STAT5	249	252	PF00017	0.512
LIG_SH2_STAT5	260	263	PF00017	0.518
LIG_SH2_STAT5	282	285	PF00017	0.422
LIG_SH2_STAT5	309	312	PF00017	0.293
LIG_SH2_STAT5	410	413	PF00017	0.488
LIG_SH2_STAT5	435	438	PF00017	0.488
LIG_SH3_1	275	281	PF00018	0.445
LIG_SH3_3	165	171	PF00018	0.586
LIG_SH3_3	258	264	PF00018	0.466
LIG_SH3_3	275	281	PF00018	0.417
LIG_SH3_3	70	76	PF00018	0.790
LIG_Sin3_3	464	471	PF02671	0.513
LIG_SUMO_SIM_par_1	319	328	PF11976	0.255
LIG_SUMO_SIM_par_1	489	494	PF11976	0.632
LIG_TRAF2_1	38	41	PF00917	0.604
LIG_UBA3_1	1	9	PF00899	0.590
MOD_CDC14_SPxK_1	133	136	PF00782	0.594
MOD_CDK_SPK_2	474	479	PF00069	0.498
MOD_CDK_SPxK_1	130	136	PF00069	0.596
MOD_CK1_1	150	156	PF00069	0.608
MOD_CK1_1	180	186	PF00069	0.530
MOD_CK1_1	82	88	PF00069	0.660
MOD_CK2_1	164	170	PF00069	0.542
MOD_CK2_1	180	186	PF00069	0.620
MOD_GlcNHglycan	106	109	PF01048	0.420
MOD_GlcNHglycan	120	123	PF01048	0.459
MOD_GlcNHglycan	17	21	PF01048	0.407
MOD_GlcNHglycan	206	209	PF01048	0.339
MOD_GlcNHglycan	49	52	PF01048	0.475
MOD_GlcNHglycan	524	527	PF01048	0.472
MOD_GlcNHglycan	63	66	PF01048	0.451
MOD_GlcNHglycan	89	92	PF01048	0.462
MOD_GlcNHglycan	94	97	PF01048	0.568
MOD_GSK3_1	126	133	PF00069	0.665
MOD_GSK3_1	146	153	PF00069	0.563
MOD_GSK3_1	200	207	PF00069	0.555
MOD_GSK3_1	233	240	PF00069	0.478
MOD_GSK3_1	305	312	PF00069	0.328
MOD_GSK3_1	470	477	PF00069	0.468
MOD_GSK3_1	5	12	PF00069	0.647
MOD_GSK3_1	522	529	PF00069	0.609
MOD_GSK3_1	71	78	PF00069	0.706
MOD_NEK2_1	16	21	PF00069	0.592
MOD_NEK2_1	204	209	PF00069	0.479
MOD_NEK2_1	233	238	PF00069	0.446
MOD_NEK2_1	4	9	PF00069	0.614
MOD_NEK2_1	426	431	PF00069	0.474
MOD_NEK2_1	450	455	PF00069	0.544
MOD_NEK2_1	470	475	PF00069	0.380
MOD_NEK2_1	54	59	PF00069	0.627
MOD_NEK2_2	390	395	PF00069	0.385
MOD_NEK2_2	98	103	PF00069	0.651
MOD_PIKK_1	177	183	PF00454	0.576
MOD_PIKK_1	82	88	PF00454	0.689
MOD_PKA_1	455	461	PF00069	0.485
MOD_PKA_1	92	98	PF00069	0.658
MOD_PKA_2	455	461	PF00069	0.456
MOD_PKA_2	55	61	PF00069	0.597
MOD_PKA_2	92	98	PF00069	0.658
MOD_Plk_1	155	161	PF00069	0.578
MOD_Plk_1	327	333	PF00069	0.266
MOD_Plk_1	426	432	PF00069	0.454
MOD_Plk_4	127	133	PF00069	0.634
MOD_Plk_4	200	206	PF00069	0.554
MOD_Plk_4	347	353	PF00069	0.288
MOD_Plk_4	382	388	PF00069	0.244
MOD_Plk_4	390	396	PF00069	0.299
MOD_ProDKin_1	130	136	PF00069	0.719
MOD_ProDKin_1	141	147	PF00069	0.599
MOD_ProDKin_1	164	170	PF00069	0.554
MOD_ProDKin_1	206	212	PF00069	0.542
MOD_ProDKin_1	412	418	PF00069	0.513
MOD_ProDKin_1	419	425	PF00069	0.462
MOD_ProDKin_1	474	480	PF00069	0.492
MOD_ProDKin_1	72	78	PF00069	0.748
MOD_SUMO_for_1	135	138	PF00179	0.597
MOD_SUMO_rev_2	362	370	PF00179	0.234
MOD_SUMO_rev_2	39	45	PF00179	0.614
TRG_DiLeu_BaEn_4	40	46	PF01217	0.554
TRG_DiLeu_BaLyEn_6	302	307	PF01217	0.422
TRG_ER_diArg_1	34	36	PF00400	0.558
TRG_ER_diArg_1	454	456	PF00400	0.545
TRG_ER_diArg_1	484	486	PF00400	0.480
TRG_ER_diArg_1	509	511	PF00400	0.612

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2C6	Leptomonas seymouri	76%	70%
A0A1X0P1D7	Trypanosomatidae	44%	94%
A4HHG8	Leishmania braziliensis	75%	100%
A4HQC7	Leishmania braziliensis	27%	100%
A4I4L7	Leishmania infantum	99%	100%
E9AE15	Leishmania major	92%	100%
E9ALR8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9AU42	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
P87234	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	83%
V5BDT8	Trypanosoma cruzi	46%	94%