Eukaryotic_translation_initiation_factor_5_putati ve/GeneDB:LmjF.34.0350

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

eukaryotic translation initiation factor 5, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IE42_LEIDO

TriTrypDb:

LdBPK_340370.1 , LdCL_340008800 , LDHU3_34.0540

Length:

378

Annotations

Annotations by Jardim et al.

Translation, eukaryotic translation initiation factor 5

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	yes	yes: 4
Forrest at al. (procyclic)	yes	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A0A3Q8IE42

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IE42

Function

Biological processes
Term	Name	Level	Count
GO:0001731	formation of translation preinitiation complex	7	1
GO:0001732	formation of cytoplasmic translation initiation complex	7	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0022618	ribonucleoprotein complex assembly	6	1
GO:0043933	protein-containing complex organization	4	1
GO:0065003	protein-containing complex assembly	5	1
GO:0071826	ribonucleoprotein complex subunit organization	5	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003743	translation initiation factor activity	4	12
GO:0005488	binding	1	12
GO:0008135	translation factor activity, RNA binding	3	12
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0005092	GDP-dissociation inhibitor activity	3	1
GO:0005515	protein binding	2	1
GO:0030234	enzyme regulator activity	2	1
GO:0030695	GTPase regulator activity	4	1
GO:0031369	translation initiation factor binding	3	1
GO:0060589	nucleoside-triphosphatase regulator activity	3	1
GO:0071074	eukaryotic initiation factor eIF2 binding	4	1
GO:0098772	molecular function regulator activity	1	1
GO:0140677	molecular function activator activity	2	1
GO:0000166	nucleotide binding	3	1
GO:0005525	GTP binding	5	1
GO:0017076	purine nucleotide binding	4	1
GO:0019001	guanyl nucleotide binding	5	1
GO:0032553	ribonucleotide binding	3	1
GO:0032555	purine ribonucleotide binding	4	1
GO:0032561	guanyl ribonucleotide binding	5	1
GO:0035639	purine ribonucleoside triphosphate binding	4	1
GO:0036094	small molecule binding	2	1
GO:0043167	ion binding	2	1
GO:0043168	anion binding	3	1
GO:0097367	carbohydrate derivative binding	2	1
GO:1901265	nucleoside phosphate binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	167	169	PF00675	0.648
CLV_NRD_NRD_1	97	99	PF00675	0.303
CLV_PCSK_KEX2_1	134	136	PF00082	0.529
CLV_PCSK_KEX2_1	269	271	PF00082	0.423
CLV_PCSK_KEX2_1	294	296	PF00082	0.376
CLV_PCSK_PC1ET2_1	134	136	PF00082	0.529
CLV_PCSK_PC1ET2_1	269	271	PF00082	0.384
CLV_PCSK_PC1ET2_1	294	296	PF00082	0.394
CLV_PCSK_SKI1_1	159	163	PF00082	0.629
CLV_PCSK_SKI1_1	206	210	PF00082	0.521
CLV_PCSK_SKI1_1	211	215	PF00082	0.535
CLV_PCSK_SKI1_1	266	270	PF00082	0.459
CLV_PCSK_SKI1_1	287	291	PF00082	0.562
CLV_PCSK_SKI1_1	301	305	PF00082	0.426
CLV_PCSK_SKI1_1	37	41	PF00082	0.283
CLV_PCSK_SKI1_1	77	81	PF00082	0.354
CLV_PCSK_SKI1_1	99	103	PF00082	0.283
DEG_CRL4_CDT2_1	287	299	PF00400	0.452
DEG_CRL4_CDT2_2	287	299	PF00400	0.452
DOC_CDC14_PxL_1	271	279	PF14671	0.555
DOC_MAPK_gen_1	199	209	PF00069	0.374
DOC_MAPK_MEF2A_6	202	209	PF00069	0.493
DOC_MAPK_MEF2A_6	319	326	PF00069	0.348
DOC_PP1_RVXF_1	204	210	PF00149	0.526
DOC_USP7_MATH_1	365	369	PF00917	0.440
DOC_USP7_UBL2_3	187	191	PF12436	0.763
DOC_USP7_UBL2_3	294	298	PF12436	0.475
DOC_WW_Pin1_4	313	318	PF00397	0.520
LIG_14-3-3_CanoR_1	142	147	PF00244	0.422
LIG_14-3-3_CanoR_1	229	237	PF00244	0.485
LIG_14-3-3_CanoR_1	270	275	PF00244	0.424
LIG_14-3-3_CanoR_1	69	78	PF00244	0.414
LIG_APCC_ABBA_1	325	330	PF00400	0.349
LIG_BIR_II_1	1	5	PF00653	0.485
LIG_deltaCOP1_diTrp_1	340	348	PF00928	0.481
LIG_FHA_1	225	231	PF00498	0.483
LIG_FHA_2	181	187	PF00498	0.774
LIG_FHA_2	25	31	PF00498	0.283
LIG_FHA_2	271	277	PF00498	0.450
LIG_FHA_2	71	77	PF00498	0.291
LIG_LIR_Apic_2	232	237	PF02991	0.550
LIG_LIR_Apic_2	238	243	PF02991	0.507
LIG_LIR_Gen_1	318	328	PF02991	0.473
LIG_LIR_Gen_1	75	85	PF02991	0.374
LIG_LIR_Gen_1	91	102	PF02991	0.270
LIG_LIR_Nem_3	14	20	PF02991	0.283
LIG_LIR_Nem_3	318	323	PF02991	0.481
LIG_LIR_Nem_3	91	97	PF02991	0.312
LIG_PCNA_yPIPBox_3	88	97	PF02747	0.271
LIG_Pex14_2	311	315	PF04695	0.366
LIG_Pex14_2	96	100	PF04695	0.268
LIG_SH2_CRK	20	24	PF00017	0.283
LIG_SH2_CRK	240	244	PF00017	0.490
LIG_SH2_CRK	94	98	PF00017	0.300
LIG_SH2_NCK_1	234	238	PF00017	0.545
LIG_SH2_STAT5	105	108	PF00017	0.418
LIG_SH2_STAT5	60	63	PF00017	0.362
LIG_SH3_2	182	187	PF14604	0.727
LIG_SH3_2	7	12	PF14604	0.387
LIG_SH3_3	176	182	PF00018	0.743
LIG_SH3_3	4	10	PF00018	0.410
LIG_UBA3_1	257	262	PF00899	0.432
MOD_CDK_SPxK_1	313	319	PF00069	0.518
MOD_CK1_1	189	195	PF00069	0.633
MOD_CK2_1	180	186	PF00069	0.610
MOD_CK2_1	24	30	PF00069	0.283
MOD_CK2_1	270	276	PF00069	0.443
MOD_Cter_Amidation	117	120	PF01082	0.362
MOD_Cter_Amidation	132	135	PF01082	0.362
MOD_GlcNHglycan	121	124	PF01048	0.383
MOD_GlcNHglycan	188	191	PF01048	0.768
MOD_GlcNHglycan	246	249	PF01048	0.507
MOD_GlcNHglycan	30	34	PF01048	0.302
MOD_GSK3_1	119	126	PF00069	0.373
MOD_GSK3_1	189	196	PF00069	0.601
MOD_GSK3_1	256	263	PF00069	0.548
MOD_GSK3_1	342	349	PF00069	0.504
MOD_N-GLC_1	313	318	PF02516	0.499
MOD_NEK2_1	213	218	PF00069	0.489
MOD_NEK2_1	244	249	PF00069	0.454
MOD_NEK2_1	257	262	PF00069	0.516
MOD_PIKK_1	229	235	PF00454	0.488
MOD_PK_1	334	340	PF00069	0.446
MOD_PKA_1	119	125	PF00069	0.330
MOD_Plk_1	334	340	PF00069	0.446
MOD_Plk_4	142	148	PF00069	0.432
MOD_Plk_4	299	305	PF00069	0.482
MOD_Plk_4	35	41	PF00069	0.283
MOD_Plk_4	365	371	PF00069	0.444
MOD_ProDKin_1	313	319	PF00069	0.518
MOD_SUMO_rev_2	152	161	PF00179	0.602
MOD_SUMO_rev_2	200	208	PF00179	0.550
MOD_SUMO_rev_2	279	289	PF00179	0.613
MOD_SUMO_rev_2	291	299	PF00179	0.521
MOD_SUMO_rev_2	340	348	PF00179	0.455
MOD_SUMO_rev_2	349	355	PF00179	0.470
MOD_SUMO_rev_2	368	376	PF00179	0.386
MOD_SUMO_rev_2	72	79	PF00179	0.287
TRG_DiLeu_BaLyEn_6	226	231	PF01217	0.497
TRG_DiLeu_BaLyEn_6	263	268	PF01217	0.447
TRG_ENDOCYTIC_2	60	63	PF00928	0.330
TRG_ENDOCYTIC_2	94	97	PF00928	0.316
TRG_Pf-PMV_PEXEL_1	211	215	PF00026	0.483
TRG_Pf-PMV_PEXEL_1	229	233	PF00026	0.483
TRG_Pf-PMV_PEXEL_1	287	291	PF00026	0.607
TRG_Pf-PMV_PEXEL_1	336	340	PF00026	0.496

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I358	Leptomonas seymouri	78%	100%
A0A0S4KIP7	Bodo saltans	40%	100%
A0A1X0P9X7	Trypanosomatidae	47%	97%
A0A422NFJ1	Trypanosoma rangeli	35%	100%
A4HAE9	Leishmania braziliensis	96%	100%
A4I9K5	Leishmania infantum	100%	100%
D0A1W7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	99%
E9B4K3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
Q4Q3H3	Leishmania major	99%	100%
V5DIM8	Trypanosoma cruzi	48%	100%