Calpain family cysteine protease-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain family cysteine protease-like protein

Species:

Leishmania donovani

UniProt:

A0A3Q8IDD4_LEIDO

TriTrypDb:

LdBPK_251540.1 , LdCL_250021100 , LDHU3_25.1910

Length:

706

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 3
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 36

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 20

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 2, no: 103
NetGPI	no	yes: 0, no: 105

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0110165	cellular anatomical entity	1	5
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0005930	axoneme	2	1
GO:0016020	membrane	2	1

Expansion

Sequence features

A0A3Q8IDD4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IDD4

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	106
GO:0006807	nitrogen compound metabolic process	2	106
GO:0008152	metabolic process	1	106
GO:0019538	protein metabolic process	3	106
GO:0043170	macromolecule metabolic process	3	106
GO:0044238	primary metabolic process	2	106
GO:0071704	organic substance metabolic process	2	106
GO:1901564	organonitrogen compound metabolic process	3	106

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	106
GO:0004175	endopeptidase activity	4	106
GO:0004197	cysteine-type endopeptidase activity	5	106
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	106
GO:0008233	peptidase activity	3	106
GO:0008234	cysteine-type peptidase activity	4	106
GO:0016787	hydrolase activity	2	106
GO:0140096	catalytic activity, acting on a protein	2	106

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	547	551	PF00656	0.512
CLV_NRD_NRD_1	139	141	PF00675	0.629
CLV_NRD_NRD_1	200	202	PF00675	0.411
CLV_NRD_NRD_1	265	267	PF00675	0.353
CLV_NRD_NRD_1	361	363	PF00675	0.491
CLV_NRD_NRD_1	494	496	PF00675	0.626
CLV_NRD_NRD_1	612	614	PF00675	0.436
CLV_NRD_NRD_1	691	693	PF00675	0.583
CLV_NRD_NRD_1	76	78	PF00675	0.561
CLV_PCSK_KEX2_1	192	194	PF00082	0.414
CLV_PCSK_KEX2_1	200	202	PF00082	0.404
CLV_PCSK_KEX2_1	494	496	PF00082	0.554
CLV_PCSK_KEX2_1	612	614	PF00082	0.496
CLV_PCSK_PC1ET2_1	192	194	PF00082	0.457
CLV_PCSK_SKI1_1	154	158	PF00082	0.573
CLV_PCSK_SKI1_1	250	254	PF00082	0.381
CLV_PCSK_SKI1_1	34	38	PF00082	0.415
CLV_PCSK_SKI1_1	363	367	PF00082	0.374
CLV_PCSK_SKI1_1	612	616	PF00082	0.574
CLV_Separin_Metazoa	74	78	PF03568	0.279
DEG_MDM2_SWIB_1	475	482	PF02201	0.569
DEG_SCF_TRCP1_1	80	85	PF00400	0.282
DOC_AGCK_PIF_2	343	348	PF00069	0.471
DOC_CKS1_1	381	386	PF01111	0.580
DOC_CYCLIN_RxL_1	360	368	PF00134	0.659
DOC_MAPK_gen_1	157	167	PF00069	0.522
DOC_MAPK_gen_1	208	216	PF00069	0.681
DOC_MAPK_gen_1	375	381	PF00069	0.563
DOC_MAPK_gen_1	600	610	PF00069	0.570
DOC_MAPK_gen_1	624	631	PF00069	0.498
DOC_MAPK_MEF2A_6	208	216	PF00069	0.673
DOC_MAPK_MEF2A_6	552	560	PF00069	0.424
DOC_PP2B_LxvP_1	608	611	PF13499	0.466
DOC_USP7_MATH_1	130	134	PF00917	0.664
DOC_USP7_MATH_1	20	24	PF00917	0.485
DOC_USP7_MATH_1	382	386	PF00917	0.569
DOC_USP7_MATH_1	437	441	PF00917	0.628
DOC_USP7_MATH_1	574	578	PF00917	0.551
DOC_USP7_MATH_1	86	90	PF00917	0.426
DOC_USP7_UBL2_3	12	16	PF12436	0.546
DOC_USP7_UBL2_3	123	127	PF12436	0.559
DOC_USP7_UBL2_3	209	213	PF12436	0.652
DOC_USP7_UBL2_3	250	254	PF12436	0.692
DOC_USP7_UBL2_3	316	320	PF12436	0.583
DOC_USP7_UBL2_3	390	394	PF12436	0.648
DOC_USP7_UBL2_3	96	100	PF12436	0.498
DOC_WW_Pin1_4	218	223	PF00397	0.562
DOC_WW_Pin1_4	24	29	PF00397	0.443
DOC_WW_Pin1_4	380	385	PF00397	0.592
DOC_WW_Pin1_4	449	454	PF00397	0.599
DOC_WW_Pin1_4	663	668	PF00397	0.481
LIG_14-3-3_CanoR_1	593	598	PF00244	0.520
LIG_14-3-3_CanoR_1	612	622	PF00244	0.540
LIG_Actin_WH2_2	374	392	PF00022	0.649
LIG_BIR_III_2	10	14	PF00653	0.751
LIG_BRCT_BRCA1_1	482	486	PF00533	0.578
LIG_BRCT_BRCA1_1	489	493	PF00533	0.574
LIG_deltaCOP1_diTrp_1	308	313	PF00928	0.649
LIG_deltaCOP1_diTrp_1	42	46	PF00928	0.355
LIG_deltaCOP1_diTrp_1	443	448	PF00928	0.579
LIG_deltaCOP1_diTrp_1	478	486	PF00928	0.587
LIG_FHA_1	281	287	PF00498	0.593
LIG_FHA_1	516	522	PF00498	0.435
LIG_FHA_1	583	589	PF00498	0.642
LIG_FHA_1	71	77	PF00498	0.521
LIG_FHA_1	83	89	PF00498	0.445
LIG_FHA_1	99	105	PF00498	0.462
LIG_FHA_2	355	361	PF00498	0.599
LIG_IRF3_LxIS_1	273	278	PF10401	0.665
LIG_LIR_Apic_2	196	202	PF02991	0.575
LIG_LIR_Gen_1	308	318	PF02991	0.574
LIG_LIR_Gen_1	324	333	PF02991	0.579
LIG_LIR_Gen_1	510	519	PF02991	0.474
LIG_LIR_Gen_1	550	560	PF02991	0.500
LIG_LIR_Gen_1	701	706	PF02991	0.707
LIG_LIR_Nem_3	278	284	PF02991	0.596
LIG_LIR_Nem_3	308	313	PF02991	0.578
LIG_LIR_Nem_3	315	321	PF02991	0.578
LIG_LIR_Nem_3	324	328	PF02991	0.581
LIG_LIR_Nem_3	341	346	PF02991	0.600
LIG_LIR_Nem_3	42	47	PF02991	0.384
LIG_LIR_Nem_3	452	458	PF02991	0.551
LIG_LIR_Nem_3	478	482	PF02991	0.568
LIG_LIR_Nem_3	501	505	PF02991	0.499
LIG_LIR_Nem_3	510	514	PF02991	0.467
LIG_LIR_Nem_3	527	533	PF02991	0.517
LIG_LIR_Nem_3	550	556	PF02991	0.513
LIG_LIR_Nem_3	596	601	PF02991	0.464
LIG_LIR_Nem_3	701	705	PF02991	0.653
LIG_LYPXL_yS_3	458	461	PF13949	0.537
LIG_MLH1_MIPbox_1	482	486	PF16413	0.629
LIG_PCNA_yPIPBox_3	267	281	PF02747	0.552
LIG_Pex14_2	348	352	PF04695	0.575
LIG_Pex14_2	475	479	PF04695	0.577
LIG_SH2_CRK	111	115	PF00017	0.488
LIG_SH2_CRK	318	322	PF00017	0.576
LIG_SH2_CRK	325	329	PF00017	0.584
LIG_SH2_CRK	505	509	PF00017	0.453
LIG_SH2_CRK	553	557	PF00017	0.500
LIG_SH2_NCK_1	318	322	PF00017	0.629
LIG_SH2_PTP2	533	536	PF00017	0.499
LIG_SH2_SRC	289	292	PF00017	0.588
LIG_SH2_SRC	627	630	PF00017	0.490
LIG_SH2_STAP1	318	322	PF00017	0.613
LIG_SH2_STAP1	503	507	PF00017	0.430
LIG_SH2_STAP1	553	557	PF00017	0.421
LIG_SH2_STAT3	332	335	PF00017	0.657
LIG_SH2_STAT3	587	590	PF00017	0.614
LIG_SH2_STAT5	166	169	PF00017	0.599
LIG_SH2_STAT5	235	238	PF00017	0.590
LIG_SH2_STAT5	289	292	PF00017	0.588
LIG_SH2_STAT5	377	380	PF00017	0.612
LIG_SH2_STAT5	45	48	PF00017	0.469
LIG_SH2_STAT5	533	536	PF00017	0.444
LIG_SH2_STAT5	587	590	PF00017	0.555
LIG_SH2_STAT5	607	610	PF00017	0.450
LIG_SH2_STAT5	627	630	PF00017	0.472
LIG_SH3_3	577	583	PF00018	0.579
LIG_SH3_4	12	19	PF00018	0.612
LIG_SUMO_SIM_anti_2	517	524	PF11976	0.402
LIG_SUMO_SIM_par_1	282	288	PF11976	0.558
LIG_SUMO_SIM_par_1	556	562	PF11976	0.478
LIG_SUMO_SIM_par_1	627	633	PF11976	0.545
LIG_TYR_ITIM	109	114	PF00017	0.286
LIG_UBA3_1	216	223	PF00899	0.480
LIG_UBA3_1	35	40	PF00899	0.452
LIG_UBA3_1	520	526	PF00899	0.327
LIG_WRC_WIRS_1	508	513	PF05994	0.610
LIG_WRC_WIRS_1	659	664	PF05994	0.552
MOD_CDK_SPK_2	218	223	PF00069	0.649
MOD_CK1_1	23	29	PF00069	0.425
MOD_CK1_1	669	675	PF00069	0.606
MOD_CK2_1	309	315	PF00069	0.572
MOD_CK2_1	437	443	PF00069	0.577
MOD_CK2_1	556	562	PF00069	0.485
MOD_CK2_1	68	74	PF00069	0.411
MOD_CMANNOS	476	479	PF00535	0.378
MOD_Cter_Amidation	693	696	PF01082	0.689
MOD_GlcNHglycan	111	114	PF01048	0.477
MOD_GlcNHglycan	132	135	PF01048	0.657
MOD_GlcNHglycan	22	25	PF01048	0.441
MOD_GlcNHglycan	277	280	PF01048	0.396
MOD_GlcNHglycan	396	399	PF01048	0.408
MOD_GlcNHglycan	487	490	PF01048	0.386
MOD_GlcNHglycan	80	83	PF01048	0.433
MOD_GSK3_1	167	174	PF00069	0.570
MOD_GSK3_1	20	27	PF00069	0.444
MOD_GSK3_1	285	292	PF00069	0.612
MOD_GSK3_1	354	361	PF00069	0.589
MOD_GSK3_1	544	551	PF00069	0.619
MOD_GSK3_1	589	596	PF00069	0.463
MOD_GSK3_1	632	639	PF00069	0.584
MOD_GSK3_1	665	672	PF00069	0.530
MOD_GSK3_1	78	85	PF00069	0.458
MOD_N-GLC_1	218	223	PF02516	0.494
MOD_N-GLC_1	275	280	PF02516	0.383
MOD_N-GLC_1	47	52	PF02516	0.427
MOD_N-GLC_1	524	529	PF02516	0.549
MOD_N-GLC_1	565	570	PF02516	0.582
MOD_N-GLC_1	574	579	PF02516	0.529
MOD_N-GLC_1	62	67	PF02516	0.427
MOD_N-GLC_1	78	83	PF02516	0.530
MOD_N-GLC_2	3	5	PF02516	0.533
MOD_NEK2_1	275	280	PF00069	0.602
MOD_NEK2_1	309	314	PF00069	0.579
MOD_NEK2_1	351	356	PF00069	0.639
MOD_NEK2_1	365	370	PF00069	0.586
MOD_NEK2_1	485	490	PF00069	0.570
MOD_NEK2_1	589	594	PF00069	0.507
MOD_NEK2_1	614	619	PF00069	0.634
MOD_NEK2_2	532	537	PF00069	0.552
MOD_NEK2_2	86	91	PF00069	0.412
MOD_NMyristoyl	1	7	PF02799	0.625
MOD_PIKK_1	172	178	PF00454	0.640
MOD_PIKK_1	223	229	PF00454	0.615
MOD_PIKK_1	260	266	PF00454	0.528
MOD_PIKK_1	47	53	PF00454	0.455
MOD_PIKK_1	525	531	PF00454	0.521
MOD_PIKK_1	565	571	PF00454	0.659
MOD_PIKK_1	630	636	PF00454	0.612
MOD_Plk_1	167	173	PF00069	0.578
MOD_Plk_4	174	180	PF00069	0.592
MOD_Plk_4	285	291	PF00069	0.615
MOD_Plk_4	414	420	PF00069	0.603
MOD_Plk_4	593	599	PF00069	0.518
MOD_Plk_4	669	675	PF00069	0.618
MOD_Plk_4	86	92	PF00069	0.445
MOD_ProDKin_1	218	224	PF00069	0.562
MOD_ProDKin_1	24	30	PF00069	0.443
MOD_ProDKin_1	380	386	PF00069	0.592
MOD_ProDKin_1	449	455	PF00069	0.599
MOD_ProDKin_1	663	669	PF00069	0.485
MOD_SUMO_for_1	11	14	PF00179	0.558
MOD_SUMO_rev_2	10	17	PF00179	0.602
MOD_SUMO_rev_2	368	378	PF00179	0.612
MOD_SUMO_rev_2	417	424	PF00179	0.625
MOD_SUMO_rev_2	547	554	PF00179	0.508
MOD_SUMO_rev_2	89	98	PF00179	0.484
TRG_DiLeu_BaEn_1	374	379	PF01217	0.658
TRG_DiLeu_BaLyEn_6	151	156	PF01217	0.379
TRG_ENDOCYTIC_2	111	114	PF00928	0.407
TRG_ENDOCYTIC_2	318	321	PF00928	0.580
TRG_ENDOCYTIC_2	325	328	PF00928	0.585
TRG_ENDOCYTIC_2	416	419	PF00928	0.603
TRG_ENDOCYTIC_2	458	461	PF00928	0.542
TRG_ENDOCYTIC_2	505	508	PF00928	0.434
TRG_ENDOCYTIC_2	533	536	PF00928	0.465
TRG_ENDOCYTIC_2	553	556	PF00928	0.470
TRG_ENDOCYTIC_2	598	601	PF00928	0.503
TRG_ENDOCYTIC_2	607	610	PF00928	0.434
TRG_ER_diArg_1	199	201	PF00400	0.606
TRG_ER_diArg_1	493	495	PF00400	0.481
TRG_ER_diArg_1	611	613	PF00400	0.538
TRG_NLS_Bipartite_1	612	628	PF00514	0.629
TRG_NLS_MonoExtC_3	361	367	PF00514	0.474
TRG_NLS_MonoExtC_3	691	696	PF00514	0.635
TRG_NLS_MonoExtN_4	623	628	PF00514	0.587
TRG_Pf-PMV_PEXEL_1	140	144	PF00026	0.672

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	36%	100%
A0A0N1I8N2	Leptomonas seymouri	28%	72%
A0A0N1IGQ2	Leptomonas seymouri	70%	100%
A0A0N1ILF1	Leptomonas seymouri	34%	100%
A0A0N1IMH1	Leptomonas seymouri	33%	90%
A0A0N1P9P1	Leptomonas seymouri	31%	83%
A0A0N1PCA9	Leptomonas seymouri	29%	96%
A0A0N1PE91	Leptomonas seymouri	23%	79%
A0A0N1PFI4	Leptomonas seymouri	32%	91%
A0A0S4JLK6	Bodo saltans	33%	87%
A0A0S4JS70	Bodo saltans	24%	92%
A0A0S4KGT2	Bodo saltans	39%	94%
A0A1X0NJ61	Trypanosomatidae	46%	100%
A0A1X0NJK2	Trypanosomatidae	33%	93%
A0A1X0NJX8	Trypanosomatidae	34%	91%
A0A1X0NKT7	Trypanosomatidae	28%	89%
A0A1X0NKX8	Trypanosomatidae	32%	86%
A0A1X0NMT3	Trypanosomatidae	33%	83%
A0A1X0NW84	Trypanosomatidae	34%	100%
A0A1X0NW85	Trypanosomatidae	31%	100%
A0A1X0NW89	Trypanosomatidae	34%	82%
A0A1X0NWA6	Trypanosomatidae	27%	81%
A0A1X0NWW1	Trypanosomatidae	34%	98%
A0A3Q8IBS3	Leishmania donovani	34%	100%
A0A3Q8IJT4	Leishmania donovani	25%	94%
A0A3S5H5A5	Leishmania donovani	31%	83%
A0A3S5ISG2	Trypanosoma rangeli	32%	91%
A0A3S7WW18	Leishmania donovani	34%	86%
A0A3S7WW41	Leishmania donovani	29%	95%
A0A3S7WW71	Leishmania donovani	35%	100%
A0A3S7X430	Leishmania donovani	33%	90%
A0A3S7X438	Leishmania donovani	29%	75%
A0A3S7X460	Leishmania donovani	35%	91%
A0A3S7X463	Leishmania donovani	28%	75%
A0A3S7X470	Leishmania donovani	32%	98%
A0A422MYU1	Trypanosoma rangeli	32%	77%
A0A422MYX0	Trypanosoma rangeli	33%	97%
A4H3W4	Leishmania braziliensis	31%	100%
A4HE81	Leishmania braziliensis	84%	100%
A4HJ14	Leishmania braziliensis	33%	100%
A4HJ21	Leishmania braziliensis	25%	79%
A4HJ22	Leishmania braziliensis	31%	100%
A4HJ23	Leishmania braziliensis	31%	97%
A4HJ24	Leishmania braziliensis	36%	100%
A4HS39	Leishmania infantum	31%	100%
A4HYN0	Leishmania infantum	34%	100%
A4HYW1	Leishmania infantum	34%	70%
A4HYW2	Leishmania infantum	34%	100%
A4HYW3	Leishmania infantum	29%	100%
A4HYW4	Leishmania infantum	26%	71%
A4I1J4	Leishmania infantum	100%	100%
A4I6E4	Leishmania infantum	32%	100%
A4I6E6	Leishmania infantum	35%	100%
A4I6F0	Leishmania infantum	29%	100%
A4I6K4	Leishmania infantum	33%	100%
A4I6K5	Leishmania infantum	28%	75%
A4I6K6	Leishmania infantum	25%	100%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	96%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	89%
C9ZN53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	83%
C9ZWY4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	88%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	82%
E8NHF1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	100%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9AIH1	Leishmania braziliensis	34%	100%
E9AIH3	Leishmania braziliensis	32%	100%
E9AIH4	Leishmania braziliensis	29%	100%
E9AIH6	Leishmania braziliensis	24%	100%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9AUQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9AUR1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	99%
E9AXM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	75%
E9B1J2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	22%	100%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
Q4Q6L7	Leishmania major	34%	100%
Q4Q6L9	Leishmania major	32%	100%
Q4Q6M0	Leishmania major	29%	100%
Q4Q6M2	Leishmania major	22%	100%
Q4Q6M3	Leishmania major	28%	76%
Q4Q6M4	Leishmania major	33%	100%
Q4Q9U3	Leishmania major	97%	100%
Q4QCS6	Leishmania major	29%	100%
Q4QCS7	Leishmania major	34%	100%
Q4QCS8	Leishmania major	33%	100%
Q4QCS9	Leishmania major	33%	100%
Q9U0T9	Leishmania major	30%	100%
V5AYJ1	Trypanosoma cruzi	35%	99%
V5B5I4	Trypanosoma cruzi	34%	84%
V5BA05	Trypanosoma cruzi	34%	100%
V5BEL3	Trypanosoma cruzi	34%	100%
V5BN20	Trypanosoma cruzi	32%	91%
V5D5V8	Trypanosoma cruzi	28%	95%
V5D9Y2	Trypanosoma cruzi	30%	83%
V5DES7	Trypanosoma cruzi	32%	89%