Eukaryotic_translation_initiation_factor_4E_putat ive/GeneDB:LmjF.28.2500

Quick info Annotations Localization Phosphorylation Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Eukaryotic translation initiation factor 4E-3

Species:

Leishmania donovani

UniProt:

A0A3Q8IDB8_LEIDO

TriTrypDb:

LdBPK_282690.1 , LdCL_280032000 , LDHU3_28.3630

Length:

349

Annotations

Annotations by Jardim et al.

Translation, eukaryotic translation initiation factor

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0010494	cytoplasmic stress granule	5	1
GO:0016281	eukaryotic translation initiation factor 4F complex	2	1
GO:0032991	protein-containing complex	1	1
GO:0035770	ribonucleoprotein granule	3	1
GO:0036464	cytoplasmic ribonucleoprotein granule	4	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0099080	supramolecular complex	2	1
GO:0110165	cellular anatomical entity	1	1

Phosphorylation

Promastigote: 105
Promastigote/Amastigote: 84

Expansion

Sequence features

A0A3Q8IDB8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IDB8

Function

Biological processes
Term	Name	Level	Count
GO:0006413	translational initiation	3	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0044237	cellular metabolic process	2	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003743	translation initiation factor activity	4	11
GO:0005488	binding	1	11
GO:0008135	translation factor activity, RNA binding	3	11
GO:0045182	translation regulator activity	1	11
GO:0090079	translation regulator activity, nucleic acid binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11
GO:0000339	RNA cap binding	5	1
GO:0000340	RNA 7-methylguanosine cap binding	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	84	88	PF00656	0.846
CLV_NRD_NRD_1	209	211	PF00675	0.395
CLV_PCSK_KEX2_1	209	211	PF00082	0.350
CLV_PCSK_SKI1_1	210	214	PF00082	0.433
CLV_PCSK_SKI1_1	242	246	PF00082	0.462
CLV_PCSK_SKI1_1	279	283	PF00082	0.444
DEG_APCC_DBOX_1	302	310	PF00400	0.596
DEG_MDM2_SWIB_1	186	193	PF02201	0.596
DEG_Nend_UBRbox_3	1	3	PF02207	0.789
DEG_SPOP_SBC_1	19	23	PF00917	0.828
DOC_MAPK_gen_1	220	229	PF00069	0.596
DOC_MAPK_gen_1	274	283	PF00069	0.587
DOC_MAPK_HePTP_8	273	285	PF00069	0.662
DOC_MAPK_JIP1_4	279	285	PF00069	0.634
DOC_MAPK_MEF2A_6	222	231	PF00069	0.613
DOC_MAPK_MEF2A_6	276	285	PF00069	0.633
DOC_PP2B_LxvP_1	193	196	PF13499	0.569
DOC_USP7_MATH_1	10	14	PF00917	0.789
DOC_USP7_MATH_1	136	140	PF00917	0.750
DOC_USP7_MATH_1	161	165	PF00917	0.685
DOC_USP7_MATH_1	19	23	PF00917	0.812
DOC_USP7_MATH_1	194	198	PF00917	0.685
DOC_USP7_MATH_1	329	333	PF00917	0.780
DOC_USP7_MATH_1	81	85	PF00917	0.879
DOC_USP7_UBL2_3	12	16	PF12436	0.861
DOC_USP7_UBL2_3	313	317	PF12436	0.596
DOC_USP7_UBL2_3	338	342	PF12436	0.799
DOC_WW_Pin1_4	162	167	PF00397	0.620
DOC_WW_Pin1_4	341	346	PF00397	0.638
LIG_14-3-3_CanoR_1	236	245	PF00244	0.685
LIG_14-3-3_CanoR_1	80	86	PF00244	0.728
LIG_BIR_III_4	14	18	PF00653	0.821
LIG_deltaCOP1_diTrp_1	184	190	PF00928	0.596
LIG_FHA_1	224	230	PF00498	0.598
LIG_FHA_1	280	286	PF00498	0.638
LIG_FHA_1	31	37	PF00498	0.664
LIG_FHA_1	328	334	PF00498	0.734
LIG_FHA_2	57	63	PF00498	0.844
LIG_FHA_2	64	70	PF00498	0.760
LIG_LIR_Apic_2	215	219	PF02991	0.662
LIG_LIR_Gen_1	204	212	PF02991	0.598
LIG_LIR_Gen_1	246	257	PF02991	0.596
LIG_LIR_Nem_3	146	150	PF02991	0.598
LIG_LIR_Nem_3	169	175	PF02991	0.635
LIG_LIR_Nem_3	184	189	PF02991	0.513
LIG_LIR_Nem_3	204	208	PF02991	0.450
LIG_PAM2_1	37	49	PF00658	0.754
LIG_Pex14_2	172	176	PF04695	0.637
LIG_Pex14_2	186	190	PF04695	0.534
LIG_SH2_SRC	192	195	PF00017	0.653
LIG_SH2_STAP1	203	207	PF00017	0.685
LIG_SH2_STAT3	8	11	PF00017	0.781
LIG_SH2_STAT5	192	195	PF00017	0.607
LIG_SH2_STAT5	203	206	PF00017	0.584
LIG_SH2_STAT5	8	11	PF00017	0.820
LIG_SH3_3	24	30	PF00018	0.832
LIG_SH3_3	42	48	PF00018	0.734
LIG_SUMO_SIM_anti_2	226	231	PF11976	0.634
LIG_SxIP_EBH_1	135	147	PF03271	0.716
LIG_WRC_WIRS_1	183	188	PF05994	0.657
MOD_CK1_1	162	168	PF00069	0.645
MOD_CK1_1	237	243	PF00069	0.669
MOD_CK1_1	55	61	PF00069	0.884
MOD_CK2_1	257	263	PF00069	0.585
MOD_CMANNOS	187	190	PF00535	0.396
MOD_GlcNHglycan	138	141	PF01048	0.730
MOD_GlcNHglycan	196	199	PF01048	0.434
MOD_GlcNHglycan	22	25	PF01048	0.867
MOD_GlcNHglycan	235	239	PF01048	0.451
MOD_GlcNHglycan	3	6	PF01048	0.836
MOD_GlcNHglycan	39	42	PF01048	0.839
MOD_GlcNHglycan	54	57	PF01048	0.879
MOD_GlcNHglycan	87	90	PF01048	0.917
MOD_GSK3_1	155	162	PF00069	0.639
MOD_GSK3_1	164	171	PF00069	0.643
MOD_GSK3_1	194	201	PF00069	0.591
MOD_GSK3_1	298	305	PF00069	0.612
MOD_GSK3_1	30	37	PF00069	0.777
MOD_GSK3_1	341	348	PF00069	0.630
MOD_GSK3_1	52	59	PF00069	0.886
MOD_GSK3_1	81	88	PF00069	0.848
MOD_N-GLC_1	162	167	PF02516	0.459
MOD_NEK2_1	298	303	PF00069	0.594
MOD_PIKK_1	102	108	PF00454	0.784
MOD_PIKK_1	30	36	PF00454	0.815
MOD_PKA_1	96	102	PF00069	0.675
MOD_PKA_2	302	308	PF00069	0.653
MOD_Plk_1	102	108	PF00069	0.759
MOD_Plk_4	182	188	PF00069	0.637
MOD_Plk_4	198	204	PF00069	0.520
MOD_ProDKin_1	162	168	PF00069	0.620
MOD_ProDKin_1	341	347	PF00069	0.632
MOD_SUMO_for_1	15	18	PF00179	0.805
MOD_SUMO_for_1	212	215	PF00179	0.641
MOD_SUMO_for_1	95	98	PF00179	0.903
MOD_SUMO_rev_2	117	124	PF00179	0.720
MOD_SUMO_rev_2	13	22	PF00179	0.823
MOD_SUMO_rev_2	206	214	PF00179	0.676
MOD_SUMO_rev_2	58	66	PF00179	0.888
MOD_SUMO_rev_2	68	73	PF00179	0.809
TRG_ENDOCYTIC_2	205	208	PF00928	0.596
TRG_ER_diArg_1	208	210	PF00400	0.549

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3S7	Leptomonas seymouri	73%	100%
A0A0S4IMC6	Bodo saltans	28%	73%
A0A1X0NR97	Trypanosomatidae	54%	73%
A0A3R7L212	Trypanosoma rangeli	51%	77%
A4HGV2	Leishmania braziliensis	88%	99%
A4I3Y2	Leishmania infantum	100%	100%
D0A951	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	79%
E9B072	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
Q4Q813	Leishmania major	97%	100%