tRNA pseudouridine synthase family protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pseudouridylate synthase-like protein

Species:

Leishmania donovani

UniProt:

A0A3Q8ICM8_LEIDO

TriTrypDb:

LdBPK_260410.1 , LdCL_260009600 , LDHU3_26.0530

Length:

696

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	5
GO:0043227	membrane-bounded organelle	3	5
GO:0043229	intracellular organelle	3	5
GO:0043231	intracellular membrane-bounded organelle	4	5
GO:0110165	cellular anatomical entity	1	5

Expansion

Sequence features

A0A3Q8ICM8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8ICM8

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	7
GO:0006139	nucleobase-containing compound metabolic process	3	7
GO:0006396	RNA processing	6	7
GO:0006399	tRNA metabolic process	7	7
GO:0006400	tRNA modification	6	5
GO:0006725	cellular aromatic compound metabolic process	3	7
GO:0006807	nitrogen compound metabolic process	2	7
GO:0008033	tRNA processing	8	7
GO:0008152	metabolic process	1	7
GO:0009451	RNA modification	5	7
GO:0009987	cellular process	1	7
GO:0016070	RNA metabolic process	5	7
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	5
GO:0034470	ncRNA processing	7	7
GO:0034641	cellular nitrogen compound metabolic process	3	7
GO:0034660	ncRNA metabolic process	6	7
GO:0043170	macromolecule metabolic process	3	7
GO:0043412	macromolecule modification	4	7
GO:0044237	cellular metabolic process	2	7
GO:0044238	primary metabolic process	2	7
GO:0046483	heterocycle metabolic process	3	7
GO:0071704	organic substance metabolic process	2	7
GO:0090304	nucleic acid metabolic process	4	7
GO:1901360	organic cyclic compound metabolic process	3	7
GO:1990481	mRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0004730	pseudouridylate synthase activity	5	8
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016829	lyase activity	2	8
GO:0016835	carbon-oxygen lyase activity	3	8
GO:0016836	hydro-lyase activity	4	8
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	330	334	PF00656	0.769
CLV_C14_Caspase3-7	681	685	PF00656	0.783
CLV_NRD_NRD_1	13	15	PF00675	0.633
CLV_NRD_NRD_1	149	151	PF00675	0.379
CLV_NRD_NRD_1	246	248	PF00675	0.524
CLV_NRD_NRD_1	380	382	PF00675	0.346
CLV_NRD_NRD_1	407	409	PF00675	0.459
CLV_NRD_NRD_1	446	448	PF00675	0.551
CLV_NRD_NRD_1	561	563	PF00675	0.608
CLV_NRD_NRD_1	691	693	PF00675	0.771
CLV_NRD_NRD_1	694	696	PF00675	0.768
CLV_NRD_NRD_1	8	10	PF00675	0.661
CLV_PCSK_FUR_1	444	448	PF00082	0.567
CLV_PCSK_FUR_1	689	693	PF00082	0.724
CLV_PCSK_KEX2_1	13	15	PF00082	0.625
CLV_PCSK_KEX2_1	149	151	PF00082	0.398
CLV_PCSK_KEX2_1	246	248	PF00082	0.538
CLV_PCSK_KEX2_1	407	409	PF00082	0.434
CLV_PCSK_KEX2_1	446	448	PF00082	0.551
CLV_PCSK_KEX2_1	653	655	PF00082	0.594
CLV_PCSK_KEX2_1	691	693	PF00082	0.771
CLV_PCSK_KEX2_1	694	696	PF00082	0.768
CLV_PCSK_KEX2_1	7	9	PF00082	0.662
CLV_PCSK_PC1ET2_1	653	655	PF00082	0.607
CLV_PCSK_PC7_1	442	448	PF00082	0.421
CLV_PCSK_PC7_1	9	15	PF00082	0.760
CLV_PCSK_SKI1_1	105	109	PF00082	0.552
CLV_PCSK_SKI1_1	153	157	PF00082	0.456
CLV_PCSK_SKI1_1	225	229	PF00082	0.549
CLV_PCSK_SKI1_1	286	290	PF00082	0.377
CLV_PCSK_SKI1_1	407	411	PF00082	0.485
CLV_PCSK_SKI1_1	515	519	PF00082	0.669
CLV_Separin_Metazoa	69	73	PF03568	0.341
DEG_APCC_DBOX_1	441	449	PF00400	0.425
DEG_Nend_UBRbox_1	1	4	PF02207	0.790
DEG_SPOP_SBC_1	338	342	PF00917	0.713
DOC_CKS1_1	409	414	PF01111	0.528
DOC_CYCLIN_yCln2_LP_2	161	164	PF00134	0.461
DOC_MAPK_DCC_7	194	204	PF00069	0.519
DOC_MAPK_FxFP_2	288	291	PF00069	0.457
DOC_MAPK_gen_1	149	158	PF00069	0.435
DOC_MAPK_gen_1	19	29	PF00069	0.430
DOC_MAPK_MEF2A_6	22	31	PF00069	0.391
DOC_MAPK_MEF2A_6	280	289	PF00069	0.383
DOC_MAPK_MEF2A_6	96	103	PF00069	0.375
DOC_PP1_RVXF_1	284	290	PF00149	0.486
DOC_PP2B_LxvP_1	101	104	PF13499	0.428
DOC_PP2B_LxvP_1	161	164	PF13499	0.424
DOC_PP4_FxxP_1	288	291	PF00568	0.406
DOC_USP7_MATH_1	216	220	PF00917	0.658
DOC_USP7_MATH_1	323	327	PF00917	0.763
DOC_USP7_MATH_1	394	398	PF00917	0.514
DOC_USP7_MATH_1	525	529	PF00917	0.502
DOC_USP7_MATH_1	567	571	PF00917	0.673
DOC_USP7_MATH_1	579	583	PF00917	0.791
DOC_WD40_RPTOR_TOS_1	251	256	PF00400	0.558
DOC_WW_Pin1_4	325	330	PF00397	0.729
DOC_WW_Pin1_4	348	353	PF00397	0.716
DOC_WW_Pin1_4	354	359	PF00397	0.579
DOC_WW_Pin1_4	401	406	PF00397	0.464
DOC_WW_Pin1_4	408	413	PF00397	0.434
DOC_WW_Pin1_4	573	578	PF00397	0.740
DOC_WW_Pin1_4	601	606	PF00397	0.782
DOC_WW_Pin1_4	633	638	PF00397	0.700
LIG_14-3-3_CanoR_1	259	269	PF00244	0.593
LIG_14-3-3_CanoR_1	324	329	PF00244	0.736
LIG_14-3-3_CanoR_1	407	412	PF00244	0.489
LIG_14-3-3_CanoR_1	84	92	PF00244	0.410
LIG_14-3-3_CanoR_1	96	102	PF00244	0.359
LIG_Actin_WH2_2	49	64	PF00022	0.435
LIG_APCC_ABBA_1	304	309	PF00400	0.705
LIG_APCC_ABBAyCdc20_2	666	672	PF00400	0.556
LIG_BIR_III_4	450	454	PF00653	0.560
LIG_BIR_III_4	662	666	PF00653	0.656
LIG_BRCT_BRCA1_1	137	141	PF00533	0.543
LIG_EH1_1	92	100	PF00400	0.374
LIG_FHA_1	126	132	PF00498	0.464
LIG_FHA_1	358	364	PF00498	0.513
LIG_FHA_1	408	414	PF00498	0.501
LIG_FHA_1	529	535	PF00498	0.428
LIG_FHA_1	617	623	PF00498	0.755
LIG_FHA_2	167	173	PF00498	0.455
LIG_FHA_2	268	274	PF00498	0.573
LIG_FHA_2	310	316	PF00498	0.663
LIG_FHA_2	325	331	PF00498	0.597
LIG_FHA_2	499	505	PF00498	0.661
LIG_FHA_2	595	601	PF00498	0.815
LIG_FHA_2	602	608	PF00498	0.732
LIG_FHA_2	634	640	PF00498	0.674
LIG_FHA_2	674	680	PF00498	0.824
LIG_LIR_Apic_2	195	200	PF02991	0.466
LIG_LIR_Gen_1	106	114	PF02991	0.472
LIG_LIR_Gen_1	166	176	PF02991	0.414
LIG_LIR_Gen_1	198	209	PF02991	0.495
LIG_LIR_Gen_1	245	255	PF02991	0.425
LIG_LIR_Gen_1	531	539	PF02991	0.523
LIG_LIR_Gen_1	627	637	PF02991	0.621
LIG_LIR_Nem_3	166	171	PF02991	0.388
LIG_LIR_Nem_3	198	204	PF02991	0.451
LIG_LIR_Nem_3	245	251	PF02991	0.400
LIG_LIR_Nem_3	36	41	PF02991	0.425
LIG_LIR_Nem_3	424	429	PF02991	0.386
LIG_LIR_Nem_3	513	517	PF02991	0.642
LIG_LIR_Nem_3	531	536	PF02991	0.368
LIG_LIR_Nem_3	627	633	PF02991	0.657
LIG_PAM2_1	356	368	PF00658	0.481
LIG_PCNA_yPIPBox_3	144	155	PF02747	0.405
LIG_Pex14_2	648	652	PF04695	0.670
LIG_RPA_C_Fungi	254	266	PF08784	0.635
LIG_SH2_CRK	154	158	PF00017	0.377
LIG_SH2_CRK	159	163	PF00017	0.394
LIG_SH2_CRK	197	201	PF00017	0.461
LIG_SH2_CRK	514	518	PF00017	0.653
LIG_SH2_GRB2like	248	251	PF00017	0.467
LIG_SH2_NCK_1	197	201	PF00017	0.420
LIG_SH2_NCK_1	660	664	PF00017	0.548
LIG_SH2_PTP2	201	204	PF00017	0.478
LIG_SH2_SRC	660	663	PF00017	0.629
LIG_SH2_STAP1	314	318	PF00017	0.635
LIG_SH2_STAP1	429	433	PF00017	0.474
LIG_SH2_STAT3	420	423	PF00017	0.496
LIG_SH2_STAT5	201	204	PF00017	0.449
LIG_SH2_STAT5	418	421	PF00017	0.372
LIG_SH2_STAT5	632	635	PF00017	0.635
LIG_SH2_STAT5	647	650	PF00017	0.517
LIG_SH3_2	516	521	PF14604	0.631
LIG_SH3_3	207	213	PF00018	0.649
LIG_SH3_3	263	269	PF00018	0.521
LIG_SH3_3	406	412	PF00018	0.430
LIG_SH3_3	513	519	PF00018	0.583
LIG_SUMO_SIM_par_1	471	476	PF11976	0.436
LIG_SxIP_EBH_1	135	149	PF03271	0.536
LIG_SxIP_EBH_1	515	528	PF03271	0.599
LIG_TRAF2_1	636	639	PF00917	0.603
LIG_TRFH_1	159	163	PF08558	0.474
LIG_TYR_ITIM	199	204	PF00017	0.505
LIG_UBA3_1	376	382	PF00899	0.357
LIG_UBA3_1	57	62	PF00899	0.457
LIG_WW_3	321	325	PF00397	0.689
MOD_CDC14_SPxK_1	404	407	PF00782	0.474
MOD_CDK_SPxK_1	401	407	PF00069	0.460
MOD_CDK_SPxxK_3	401	408	PF00069	0.482
MOD_CK1_1	219	225	PF00069	0.571
MOD_CK1_1	240	246	PF00069	0.500
MOD_CK1_1	257	263	PF00069	0.519
MOD_CK1_1	340	346	PF00069	0.703
MOD_CK1_1	357	363	PF00069	0.583
MOD_CK1_1	528	534	PF00069	0.424
MOD_CK1_1	614	620	PF00069	0.741
MOD_CK1_1	85	91	PF00069	0.415
MOD_CK2_1	267	273	PF00069	0.506
MOD_CK2_1	307	313	PF00069	0.633
MOD_CK2_1	324	330	PF00069	0.561
MOD_CK2_1	394	400	PF00069	0.537
MOD_CK2_1	498	504	PF00069	0.635
MOD_CK2_1	594	600	PF00069	0.708
MOD_CK2_1	633	639	PF00069	0.679
MOD_DYRK1A_RPxSP_1	408	412	PF00069	0.528
MOD_GlcNHglycan	137	140	PF01048	0.569
MOD_GlcNHglycan	218	221	PF01048	0.614
MOD_GlcNHglycan	259	262	PF01048	0.637
MOD_GlcNHglycan	33	36	PF01048	0.482
MOD_GlcNHglycan	569	572	PF01048	0.597
MOD_GlcNHglycan	615	619	PF01048	0.756
MOD_GSK3_1	325	332	PF00069	0.689
MOD_GSK3_1	333	340	PF00069	0.676
MOD_GSK3_1	343	350	PF00069	0.585
MOD_GSK3_1	354	361	PF00069	0.559
MOD_GSK3_1	494	501	PF00069	0.702
MOD_GSK3_1	524	531	PF00069	0.568
MOD_N-GLC_1	249	254	PF02516	0.403
MOD_N-GLC_1	257	262	PF02516	0.539
MOD_N-GLC_1	358	363	PF02516	0.511
MOD_N-GLC_1	594	599	PF02516	0.775
MOD_NEK2_1	135	140	PF00069	0.522
MOD_NEK2_1	242	247	PF00069	0.437
MOD_NEK2_1	337	342	PF00069	0.799
MOD_NEK2_1	421	426	PF00069	0.375
MOD_NEK2_1	566	571	PF00069	0.539
MOD_NEK2_1	82	87	PF00069	0.455
MOD_PIKK_1	192	198	PF00454	0.567
MOD_PKA_1	407	413	PF00069	0.454
MOD_PKA_2	323	329	PF00069	0.736
MOD_PKA_2	394	400	PF00069	0.430
MOD_PKA_2	407	413	PF00069	0.456
MOD_PKB_1	492	500	PF00069	0.670
MOD_Plk_1	249	255	PF00069	0.447
MOD_Plk_1	358	364	PF00069	0.525
MOD_Plk_1	429	435	PF00069	0.554
MOD_Plk_1	528	534	PF00069	0.424
MOD_Plk_1	594	600	PF00069	0.723
MOD_Plk_1	638	644	PF00069	0.610
MOD_Plk_2-3	309	315	PF00069	0.617
MOD_Plk_2-3	673	679	PF00069	0.721
MOD_Plk_4	137	143	PF00069	0.514
MOD_Plk_4	219	225	PF00069	0.585
MOD_Plk_4	371	377	PF00069	0.371
MOD_Plk_4	383	389	PF00069	0.346
MOD_Plk_4	394	400	PF00069	0.383
MOD_Plk_4	528	534	PF00069	0.467
MOD_Plk_4	647	653	PF00069	0.615
MOD_Plk_4	664	670	PF00069	0.659
MOD_Plk_4	97	103	PF00069	0.480
MOD_ProDKin_1	325	331	PF00069	0.731
MOD_ProDKin_1	348	354	PF00069	0.710
MOD_ProDKin_1	401	407	PF00069	0.469
MOD_ProDKin_1	408	414	PF00069	0.430
MOD_ProDKin_1	573	579	PF00069	0.750
MOD_ProDKin_1	601	607	PF00069	0.784
MOD_ProDKin_1	633	639	PF00069	0.697
MOD_SUMO_rev_2	221	229	PF00179	0.465
MOD_SUMO_rev_2	510	517	PF00179	0.682
MOD_SUMO_rev_2	586	594	PF00179	0.731
TRG_DiLeu_BaEn_2	370	376	PF01217	0.361
TRG_DiLeu_BaEn_2	528	534	PF01217	0.399
TRG_DiLeu_BaLyEn_6	532	537	PF01217	0.518
TRG_ENDOCYTIC_2	152	155	PF00928	0.362
TRG_ENDOCYTIC_2	159	162	PF00928	0.340
TRG_ENDOCYTIC_2	201	204	PF00928	0.449
TRG_ENDOCYTIC_2	248	251	PF00928	0.467
TRG_ENDOCYTIC_2	418	421	PF00928	0.399
TRG_ENDOCYTIC_2	514	517	PF00928	0.648
TRG_ER_diArg_1	12	14	PF00400	0.651
TRG_ER_diArg_1	149	151	PF00400	0.398
TRG_ER_diArg_1	246	248	PF00400	0.543
TRG_ER_diArg_1	407	409	PF00400	0.459
TRG_ER_diArg_1	442	445	PF00400	0.603
TRG_ER_diArg_1	446	448	PF00400	0.559
TRG_ER_diArg_1	654	657	PF00400	0.579
TRG_ER_diArg_1	689	692	PF00400	0.780
TRG_ER_diArg_1	694	696	PF00400	0.752
TRG_ER_diArg_1	7	9	PF00400	0.700
TRG_ER_diArg_1	71	74	PF00400	0.512
TRG_NLS_MonoExtC_3	652	657	PF00514	0.635
TRG_Pf-PMV_PEXEL_1	39	44	PF00026	0.444
TRG_Pf-PMV_PEXEL_1	554	558	PF00026	0.581

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5J6	Leptomonas seymouri	59%	100%
A0A0S4JH91	Bodo saltans	26%	100%
A0A0S4KL20	Bodo saltans	36%	94%
A0A1X0NSY0	Trypanosomatidae	40%	100%
A0A3R7KTM1	Trypanosoma rangeli	40%	100%
A4HEP8	Leishmania braziliensis	79%	100%
A4I1X8	Leishmania infantum	99%	100%
C9ZS41	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9AY24	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
Q4Q9E9	Leishmania major	94%	100%