Eukaryotic release factor 3, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

eukaryotic release factor 3, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8ICH2_LEIDO

TriTrypDb:

LdBPK_111160.1 , LdCL_110017500 , LDHU3_11.1390

Length:

755

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 2
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 6

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0018444	translation release factor complex	2	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3Q8ICH2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8ICH2

Function

Biological processes
Term	Name	Level	Count
GO:0006412	translation	4	1
GO:0006518	peptide metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0019538	protein metabolic process	3	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034645	obsolete cellular macromolecule biosynthetic process	4	1
GO:0043043	peptide biosynthetic process	5	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043603	amide metabolic process	3	1
GO:0043604	amide biosynthetic process	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	10
GO:0003824	catalytic activity	1	10
GO:0003924	GTPase activity	7	10
GO:0005488	binding	1	10
GO:0005525	GTP binding	5	10
GO:0016462	pyrophosphatase activity	5	10
GO:0016787	hydrolase activity	2	10
GO:0016817	hydrolase activity, acting on acid anhydrides	3	10
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	10
GO:0017076	purine nucleotide binding	4	10
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	10
GO:0019001	guanyl nucleotide binding	5	10
GO:0032553	ribonucleotide binding	3	10
GO:0032555	purine ribonucleotide binding	4	10
GO:0032561	guanyl ribonucleotide binding	5	10
GO:0035639	purine ribonucleoside triphosphate binding	4	10
GO:0036094	small molecule binding	2	10
GO:0043167	ion binding	2	10
GO:0043168	anion binding	3	10
GO:0097159	organic cyclic compound binding	2	10
GO:0097367	carbohydrate derivative binding	2	10
GO:1901265	nucleoside phosphate binding	3	10
GO:1901363	heterocyclic compound binding	2	10
GO:0003676	nucleic acid binding	3	1
GO:0003747	translation release factor activity	5	1
GO:0008079	translation termination factor activity	4	1
GO:0008135	translation factor activity, RNA binding	3	1
GO:0045182	translation regulator activity	1	1
GO:0090079	translation regulator activity, nucleic acid binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	170	174	PF00656	0.709
CLV_C14_Caspase3-7	399	403	PF00656	0.427
CLV_NRD_NRD_1	292	294	PF00675	0.602
CLV_NRD_NRD_1	354	356	PF00675	0.227
CLV_NRD_NRD_1	396	398	PF00675	0.227
CLV_NRD_NRD_1	681	683	PF00675	0.308
CLV_PCSK_KEX2_1	292	294	PF00082	0.602
CLV_PCSK_KEX2_1	312	314	PF00082	0.337
CLV_PCSK_KEX2_1	354	356	PF00082	0.227
CLV_PCSK_KEX2_1	395	397	PF00082	0.228
CLV_PCSK_PC1ET2_1	312	314	PF00082	0.600
CLV_PCSK_PC1ET2_1	395	397	PF00082	0.225
CLV_PCSK_SKI1_1	380	384	PF00082	0.227
CLV_PCSK_SKI1_1	396	400	PF00082	0.227
CLV_PCSK_SKI1_1	484	488	PF00082	0.227
CLV_PCSK_SKI1_1	659	663	PF00082	0.227
CLV_PCSK_SKI1_1	724	728	PF00082	0.227
DEG_SPOP_SBC_1	261	265	PF00917	0.642
DOC_ANK_TNKS_1	731	738	PF00023	0.508
DOC_CKS1_1	146	151	PF01111	0.571
DOC_CKS1_1	236	241	PF01111	0.712
DOC_CYCLIN_yCln2_LP_2	707	713	PF00134	0.508
DOC_MAPK_gen_1	191	200	PF00069	0.581
DOC_MAPK_gen_1	202	208	PF00069	0.599
DOC_MAPK_gen_1	312	323	PF00069	0.294
DOC_MAPK_gen_1	496	505	PF00069	0.445
DOC_MAPK_gen_1	526	533	PF00069	0.491
DOC_MAPK_MEF2A_6	732	740	PF00069	0.450
DOC_PP1_RVXF_1	496	503	PF00149	0.427
DOC_PP1_RVXF_1	634	641	PF00149	0.383
DOC_PP2B_LxvP_1	401	404	PF13499	0.427
DOC_PP2B_LxvP_1	537	540	PF13499	0.424
DOC_PP4_FxxP_1	549	552	PF00568	0.407
DOC_USP7_MATH_1	160	164	PF00917	0.700
DOC_USP7_MATH_1	167	171	PF00917	0.639
DOC_USP7_MATH_1	220	224	PF00917	0.765
DOC_USP7_MATH_1	234	238	PF00917	0.738
DOC_USP7_MATH_1	329	333	PF00917	0.427
DOC_USP7_MATH_1	64	68	PF00917	0.697
DOC_USP7_MATH_2	213	219	PF00917	0.638
DOC_USP7_UBL2_3	282	286	PF12436	0.640
DOC_USP7_UBL2_3	308	312	PF12436	0.583
DOC_USP7_UBL2_3	558	562	PF12436	0.499
DOC_USP7_UBL2_3	675	679	PF12436	0.427
DOC_USP7_UBL2_3	717	721	PF12436	0.427
DOC_WW_Pin1_4	145	150	PF00397	0.655
DOC_WW_Pin1_4	15	20	PF00397	0.667
DOC_WW_Pin1_4	218	223	PF00397	0.738
DOC_WW_Pin1_4	235	240	PF00397	0.775
DOC_WW_Pin1_4	245	250	PF00397	0.766
DOC_WW_Pin1_4	285	290	PF00397	0.566
LIG_14-3-3_CanoR_1	396	401	PF00244	0.469
LIG_APCC_ABBA_1	713	718	PF00400	0.470
LIG_APCC_ABBAyCdc20_2	80	86	PF00400	0.645
LIG_BIR_II_1	1	5	PF00653	0.574
LIG_BIR_III_2	233	237	PF00653	0.622
LIG_BIR_III_4	402	406	PF00653	0.427
LIG_BRCT_BRCA1_1	597	601	PF00533	0.466
LIG_BRCT_BRCA1_2	597	603	PF00533	0.458
LIG_FHA_1	195	201	PF00498	0.613
LIG_FHA_1	451	457	PF00498	0.427
LIG_FHA_1	682	688	PF00498	0.427
LIG_FHA_1	731	737	PF00498	0.427
LIG_FHA_2	209	215	PF00498	0.693
LIG_FHA_2	222	228	PF00498	0.794
LIG_FHA_2	257	263	PF00498	0.757
LIG_FHA_2	278	284	PF00498	0.619
LIG_FHA_2	286	292	PF00498	0.508
LIG_FHA_2	397	403	PF00498	0.427
LIG_FHA_2	416	422	PF00498	0.427
LIG_FHA_2	434	440	PF00498	0.481
LIG_FHA_2	527	533	PF00498	0.469
LIG_FHA_2	542	548	PF00498	0.385
LIG_FHA_2	583	589	PF00498	0.420
LIG_FHA_2	597	603	PF00498	0.323
LIG_FXI_DFP_1	716	720	PF00024	0.245
LIG_IRF3_LxIS_1	422	428	PF10401	0.427
LIG_LIR_Apic_2	2	6	PF02991	0.595
LIG_LIR_Apic_2	546	552	PF02991	0.429
LIG_LIR_Gen_1	366	373	PF02991	0.427
LIG_LIR_Gen_1	474	483	PF02991	0.445
LIG_LIR_Gen_1	670	680	PF02991	0.427
LIG_LIR_Gen_1	718	727	PF02991	0.427
LIG_LIR_Nem_3	366	370	PF02991	0.436
LIG_LIR_Nem_3	474	479	PF02991	0.445
LIG_LIR_Nem_3	563	569	PF02991	0.448
LIG_LIR_Nem_3	670	676	PF02991	0.427
LIG_LIR_Nem_3	718	722	PF02991	0.508
LIG_PCNA_APIM_2	722	728	PF02747	0.427
LIG_Pex14_1	365	369	PF04695	0.427
LIG_Pex14_2	319	323	PF04695	0.445
LIG_Pex14_2	434	438	PF04695	0.427
LIG_REV1ctd_RIR_1	638	648	PF16727	0.508
LIG_SH2_CRK	100	104	PF00017	0.677
LIG_SH2_CRK	367	371	PF00017	0.427
LIG_SH2_GRB2like	24	27	PF00017	0.616
LIG_SH2_GRB2like	63	66	PF00017	0.618
LIG_SH2_SRC	367	370	PF00017	0.427
LIG_SH2_SRC	84	87	PF00017	0.699
LIG_SH2_STAP1	367	371	PF00017	0.427
LIG_SH2_STAT3	122	125	PF00017	0.712
LIG_SH2_STAT3	139	142	PF00017	0.418
LIG_SH2_STAT3	28	31	PF00017	0.625
LIG_SH2_STAT3	49	52	PF00017	0.532
LIG_SH2_STAT3	74	77	PF00017	0.694
LIG_SH2_STAT5	17	20	PF00017	0.678
LIG_SH2_STAT5	369	372	PF00017	0.427
LIG_SH2_STAT5	390	393	PF00017	0.428
LIG_SH2_STAT5	626	629	PF00017	0.334
LIG_SH3_3	143	149	PF00018	0.560
LIG_SH3_3	184	190	PF00018	0.582
LIG_SH3_3	233	239	PF00018	0.728
LIG_SH3_3	709	715	PF00018	0.481
LIG_SUMO_SIM_par_1	423	429	PF11976	0.427
LIG_SUMO_SIM_par_1	644	649	PF11976	0.427
LIG_SUMO_SIM_par_1	708	714	PF11976	0.508
LIG_SxIP_EBH_1	624	636	PF03271	0.368
LIG_TRAF2_1	212	215	PF00917	0.686
LIG_TRAF2_1	249	252	PF00917	0.638
LIG_TRAF2_1	288	291	PF00917	0.509
LIG_TRAF2_1	374	377	PF00917	0.470
LIG_TRAF2_1	666	669	PF00917	0.427
LIG_UBA3_1	459	465	PF00899	0.445
LIG_UBA3_1	530	538	PF00899	0.508
LIG_UBA3_1	676	683	PF00899	0.508
MOD_CDK_SPxxK_3	285	292	PF00069	0.635
MOD_CK1_1	183	189	PF00069	0.661
MOD_CK1_1	218	224	PF00069	0.741
MOD_CK1_1	332	338	PF00069	0.427
MOD_CK1_1	428	434	PF00069	0.445
MOD_CK1_1	595	601	PF00069	0.447
MOD_CK1_1	630	636	PF00069	0.345
MOD_CK1_1	655	661	PF00069	0.508
MOD_CK2_1	208	214	PF00069	0.690
MOD_CK2_1	221	227	PF00069	0.621
MOD_CK2_1	256	262	PF00069	0.662
MOD_CK2_1	285	291	PF00069	0.530
MOD_CK2_1	371	377	PF00069	0.427
MOD_CK2_1	433	439	PF00069	0.435
MOD_CK2_1	526	532	PF00069	0.448
MOD_CK2_1	539	545	PF00069	0.348
MOD_CK2_1	582	588	PF00069	0.372
MOD_CK2_1	596	602	PF00069	0.357
MOD_CK2_1	663	669	PF00069	0.508
MOD_CK2_1	742	748	PF00069	0.427
MOD_Cter_Amidation	200	203	PF01082	0.605
MOD_GlcNHglycan	110	113	PF01048	0.716
MOD_GlcNHglycan	169	172	PF01048	0.682
MOD_GlcNHglycan	182	185	PF01048	0.640
MOD_GlcNHglycan	254	257	PF01048	0.706
MOD_GlcNHglycan	268	271	PF01048	0.571
MOD_GlcNHglycan	334	337	PF01048	0.227
MOD_GlcNHglycan	570	573	PF01048	0.328
MOD_GlcNHglycan	60	63	PF01048	0.517
MOD_GlcNHglycan	654	657	PF01048	0.308
MOD_GlcNHglycan	665	668	PF01048	0.308
MOD_GSK3_1	218	225	PF00069	0.643
MOD_GSK3_1	252	259	PF00069	0.776
MOD_GSK3_1	262	269	PF00069	0.669
MOD_GSK3_1	376	383	PF00069	0.445
MOD_GSK3_1	592	599	PF00069	0.369
MOD_GSK3_1	626	633	PF00069	0.340
MOD_GSK3_1	742	749	PF00069	0.433
MOD_GSK3_1	9	16	PF00069	0.520
MOD_N-GLC_1	64	69	PF02516	0.621
MOD_NEK2_1	425	430	PF00069	0.427
MOD_NEK2_1	450	455	PF00069	0.441
MOD_NEK2_1	742	747	PF00069	0.415
MOD_PIKK_1	215	221	PF00454	0.644
MOD_PIKK_1	256	262	PF00454	0.749
MOD_PIKK_1	415	421	PF00454	0.427
MOD_PKA_1	396	402	PF00069	0.427
MOD_PKA_2	383	389	PF00069	0.427
MOD_PKA_2	396	402	PF00069	0.427
MOD_PKA_2	681	687	PF00069	0.508
MOD_PKB_1	302	310	PF00069	0.621
MOD_Plk_1	669	675	PF00069	0.445
MOD_Plk_2-3	541	547	PF00069	0.421
MOD_Plk_2-3	592	598	PF00069	0.364
MOD_Plk_4	183	189	PF00069	0.520
MOD_Plk_4	396	402	PF00069	0.438
MOD_Plk_4	409	415	PF00069	0.421
MOD_Plk_4	526	532	PF00069	0.445
MOD_ProDKin_1	145	151	PF00069	0.656
MOD_ProDKin_1	15	21	PF00069	0.668
MOD_ProDKin_1	218	224	PF00069	0.740
MOD_ProDKin_1	235	241	PF00069	0.778
MOD_ProDKin_1	245	251	PF00069	0.765
MOD_ProDKin_1	285	291	PF00069	0.560
MOD_SUMO_for_1	228	231	PF00179	0.653
MOD_SUMO_for_1	296	299	PF00179	0.524
MOD_SUMO_rev_2	221	230	PF00179	0.720
MOD_SUMO_rev_2	467	472	PF00179	0.487
MOD_SUMO_rev_2	532	540	PF00179	0.522
MOD_SUMO_rev_2	559	563	PF00179	0.493
TRG_DiLeu_BaEn_1	446	451	PF01217	0.427
TRG_ENDOCYTIC_2	367	370	PF00928	0.434
TRG_ENDOCYTIC_2	476	479	PF00928	0.427
TRG_ENDOCYTIC_2	566	569	PF00928	0.340
TRG_ER_diArg_1	353	355	PF00400	0.427
TRG_ER_diArg_1	396	398	PF00400	0.426
TRG_NLS_Bipartite_1	191	206	PF00514	0.684
TRG_NLS_MonoExtN_4	190	195	PF00514	0.564
TRG_Pf-PMV_PEXEL_1	615	619	PF00026	0.441

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PAZ5	Leptomonas seymouri	80%	100%
A0A1X0NV54	Trypanosomatidae	71%	100%
A0A3R7MLL0	Trypanosoma rangeli	70%	100%
A0A3S7X9T0	Leishmania donovani	30%	100%
A4H6P3	Leishmania braziliensis	89%	100%
A4HN87	Leishmania braziliensis	31%	100%
A4HV24	Leishmania infantum	99%	100%
A4IBV5	Leishmania infantum	30%	100%
C9ZYQ4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
D0A7C5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	100%
E9AFP2	Leishmania major	32%	100%
E9AGP5	Leishmania infantum	36%	100%
E9ANQ6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
E9B6U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
O13354	Candida albicans	41%	100%
O74718	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	39%	100%
P23637	Ogataea pini	40%	100%
Q4QGW5	Leishmania major	94%	100%
Q5R6Y0	Pongo abelii	37%	100%
Q69ZS7	Mus musculus	35%	100%
Q6AXM7	Rattus norvegicus	36%	100%
Q9HGI6	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	39%	100%
Q9HGI7	Candida maltosa	38%	100%
Q9W074	Drosophila melanogaster	35%	100%
Q9Y450	Homo sapiens	37%	100%