tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Methyltransferase TYW3, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IBW6_LEIDO

TriTrypDb:

LdBPK_090710.1 , LdCL_090012200 , LDHU3_09.0850

Length:

352

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

A0A3Q8IBW6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IBW6

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006396	RNA processing	6	13
GO:0006399	tRNA metabolic process	7	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008033	tRNA processing	8	13
GO:0008152	metabolic process	1	13
GO:0009987	cellular process	1	13
GO:0016070	RNA metabolic process	5	13
GO:0032259	methylation	2	13
GO:0034470	ncRNA processing	7	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0034660	ncRNA metabolic process	6	13
GO:0043170	macromolecule metabolic process	3	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0046483	heterocycle metabolic process	3	13
GO:0071704	organic substance metabolic process	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13
GO:0001510	RNA methylation	4	2
GO:0006400	tRNA modification	6	2
GO:0009451	RNA modification	5	2
GO:0030488	tRNA methylation	5	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0008168	methyltransferase activity	4	13
GO:0016740	transferase activity	2	13
GO:0016741	transferase activity, transferring one-carbon groups	3	13
GO:0008173	RNA methyltransferase activity	4	2
GO:0008175	tRNA methyltransferase activity	5	2
GO:0140098	catalytic activity, acting on RNA	3	2
GO:0140101	catalytic activity, acting on a tRNA	4	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	31	33	PF00675	0.686
CLV_PCSK_FUR_1	139	143	PF00082	0.424
CLV_PCSK_KEX2_1	141	143	PF00082	0.304
CLV_PCSK_PC1ET2_1	141	143	PF00082	0.300
CLV_PCSK_PC7_1	137	143	PF00082	0.189
CLV_PCSK_SKI1_1	114	118	PF00082	0.302
DEG_SCF_TRCP1_1	274	280	PF00400	0.351
DEG_SPOP_SBC_1	106	110	PF00917	0.364
DOC_CKS1_1	174	179	PF01111	0.382
DOC_CYCLIN_yCln2_LP_2	301	307	PF00134	0.282
DOC_MAPK_MEF2A_6	242	249	PF00069	0.422
DOC_MAPK_MEF2A_6	294	303	PF00069	0.273
DOC_PP2B_LxvP_1	22	25	PF13499	0.552
DOC_PP2B_LxvP_1	237	240	PF13499	0.400
DOC_PP2B_LxvP_1	301	304	PF13499	0.273
DOC_PP2B_LxvP_1	45	48	PF13499	0.696
DOC_USP7_MATH_1	195	199	PF00917	0.388
DOC_USP7_MATH_1	227	231	PF00917	0.446
DOC_USP7_MATH_1	270	274	PF00917	0.338
DOC_USP7_MATH_1	84	88	PF00917	0.469
DOC_USP7_UBL2_3	29	33	PF12436	0.660
DOC_USP7_UBL2_3	58	62	PF12436	0.458
DOC_WW_Pin1_4	132	137	PF00397	0.416
DOC_WW_Pin1_4	173	178	PF00397	0.345
DOC_WW_Pin1_4	80	85	PF00397	0.257
LIG_14-3-3_CanoR_1	242	248	PF00244	0.383
LIG_14-3-3_CanoR_1	257	262	PF00244	0.274
LIG_BIR_II_1	1	5	PF00653	0.697
LIG_BIR_III_2	18	22	PF00653	0.557
LIG_CtBP_PxDLS_1	160	164	PF00389	0.310
LIG_FHA_1	12	18	PF00498	0.749
LIG_FHA_1	242	248	PF00498	0.383
LIG_FHA_2	173	179	PF00498	0.348
LIG_FHA_2	99	105	PF00498	0.279
LIG_LYPXL_SIV_4	318	326	PF13949	0.382
LIG_PCNA_PIPBox_1	163	172	PF02747	0.400
LIG_PDZ_Class_2	347	352	PF00595	0.433
LIG_Rb_pABgroove_1	210	218	PF01858	0.244
LIG_SH2_STAT5	105	108	PF00017	0.297
LIG_SH2_STAT5	319	322	PF00017	0.278
LIG_SH3_3	14	20	PF00018	0.651
LIG_SH3_3	171	177	PF00018	0.350
LIG_SH3_3	25	31	PF00018	0.449
LIG_SH3_3	279	285	PF00018	0.260
LIG_SH3_4	29	36	PF00018	0.682
LIG_SUMO_SIM_par_1	302	308	PF11976	0.250
LIG_UBA3_1	117	122	PF00899	0.382
LIG_UBA3_1	323	330	PF00899	0.257
LIG_WW_3	29	33	PF00397	0.712
LIG_WW_3	34	38	PF00397	0.713
MOD_CDK_SPK_2	132	137	PF00069	0.392
MOD_CDK_SPK_2	80	85	PF00069	0.257
MOD_CDK_SPxxK_3	132	139	PF00069	0.405
MOD_CK1_1	109	115	PF00069	0.260
MOD_CK1_1	23	29	PF00069	0.621
MOD_CK2_1	172	178	PF00069	0.378
MOD_CK2_1	227	233	PF00069	0.431
MOD_Cter_Amidation	285	288	PF01082	0.273
MOD_GlcNHglycan	111	114	PF01048	0.257
MOD_GlcNHglycan	197	200	PF01048	0.387
MOD_GlcNHglycan	22	25	PF01048	0.432
MOD_GlcNHglycan	229	232	PF01048	0.288
MOD_GlcNHglycan	268	271	PF01048	0.319
MOD_GlcNHglycan	274	277	PF01048	0.358
MOD_GlcNHglycan	279	282	PF01048	0.390
MOD_GlcNHglycan	52	55	PF01048	0.493
MOD_GlcNHglycan	7	10	PF01048	0.577
MOD_GSK3_1	1	8	PF00069	0.626
MOD_GSK3_1	105	112	PF00069	0.318
MOD_GSK3_1	117	124	PF00069	0.295
MOD_GSK3_1	193	200	PF00069	0.362
MOD_GSK3_1	266	273	PF00069	0.298
MOD_GSK3_1	80	87	PF00069	0.387
MOD_LATS_1	255	261	PF00433	0.189
MOD_N-GLC_2	78	80	PF02516	0.273
MOD_NEK2_1	1	6	PF00069	0.653
MOD_NEK2_1	117	122	PF00069	0.236
MOD_NEK2_1	266	271	PF00069	0.323
MOD_NEK2_1	341	346	PF00069	0.394
MOD_NEK2_2	243	248	PF00069	0.292
MOD_PIKK_1	312	318	PF00454	0.425
MOD_PKA_2	227	233	PF00069	0.370
MOD_PKA_2	241	247	PF00069	0.358
MOD_PKA_2	277	283	PF00069	0.278
MOD_Plk_1	341	347	PF00069	0.353
MOD_Plk_4	207	213	PF00069	0.244
MOD_ProDKin_1	132	138	PF00069	0.416
MOD_ProDKin_1	173	179	PF00069	0.345
MOD_ProDKin_1	80	86	PF00069	0.257
MOD_SUMO_for_1	247	250	PF00179	0.306
MOD_SUMO_rev_2	329	338	PF00179	0.326
MOD_SUMO_rev_2	39	43	PF00179	0.541
MOD_SUMO_rev_2	83	93	PF00179	0.292
TRG_DiLeu_BaEn_1	262	267	PF01217	0.310
TRG_DiLeu_BaLyEn_6	336	341	PF01217	0.408
TRG_DiLeu_BaLyEn_6	41	46	PF01217	0.584
TRG_ER_diArg_1	136	139	PF00400	0.468
TRG_ER_diArg_1	256	259	PF00400	0.189
TRG_NLS_MonoExtC_3	31	36	PF00514	0.724
TRG_NLS_MonoExtN_4	29	36	PF00514	0.713

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P754	Leptomonas seymouri	64%	100%
A0A0N1PB24	Leptomonas seymouri	26%	100%
A0A0S4JMK0	Bodo saltans	43%	100%
A0A1X0NNC3	Trypanosomatidae	51%	100%
A0A3S5IRY7	Trypanosoma rangeli	45%	100%
A4H5P7	Leishmania braziliensis	82%	100%
A4HTY7	Leishmania infantum	100%	100%
D0A9E3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	99%
E9AMR7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
P53177	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	100%
Q4Q4P7	Leishmania major	29%	97%
Q4QHV8	Leishmania major	92%	100%
V5DJH9	Trypanosoma cruzi	48%	91%