Thiamine pyrophosphokinase, vitamin B1 binding domain containing protein, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Thiamin pyrophosphokinase, vitamin B1 binding domain containing protein, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IBS6_LEIDO

TriTrypDb:

LdBPK_131140.1 , LdCL_130016700 , LDHU3_13.1400

Length:

549

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A0A3Q8IBS6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IBS6

Function

Biological processes
Term	Name	Level	Count
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006766	vitamin metabolic process	3	11
GO:0006767	water-soluble vitamin metabolic process	4	11
GO:0006790	sulfur compound metabolic process	3	11
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009110	vitamin biosynthetic process	4	11
GO:0009229	thiamine diphosphate biosynthetic process	5	11
GO:0009987	cellular process	1	11
GO:0016310	phosphorylation	5	10
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019637	organophosphate metabolic process	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0042357	thiamine diphosphate metabolic process	4	11
GO:0042364	water-soluble vitamin biosynthetic process	5	11
GO:0042723	thiamine-containing compound metabolic process	4	11
GO:0042724	thiamine-containing compound biosynthetic process	5	11
GO:0044237	cellular metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0044272	sulfur compound biosynthetic process	4	11
GO:0044281	small molecule metabolic process	2	11
GO:0044283	small molecule biosynthetic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0072527	pyrimidine-containing compound metabolic process	4	11
GO:0072528	pyrimidine-containing compound biosynthetic process	5	11
GO:0090407	organophosphate biosynthetic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901566	organonitrogen compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004788	thiamine diphosphokinase activity	5	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016301	kinase activity	4	10
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016778	diphosphotransferase activity	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0019842	vitamin binding	3	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0030975	thiamine binding	3	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0043169	cation binding	3	11
GO:0043178	alcohol binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:1901681	sulfur compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	173	177	PF00656	0.442
CLV_NRD_NRD_1	18	20	PF00675	0.424
CLV_NRD_NRD_1	192	194	PF00675	0.616
CLV_NRD_NRD_1	226	228	PF00675	0.511
CLV_NRD_NRD_1	335	337	PF00675	0.492
CLV_PCSK_FUR_1	224	228	PF00082	0.508
CLV_PCSK_FUR_1	333	337	PF00082	0.355
CLV_PCSK_KEX2_1	117	119	PF00082	0.379
CLV_PCSK_KEX2_1	122	124	PF00082	0.416
CLV_PCSK_KEX2_1	191	193	PF00082	0.718
CLV_PCSK_KEX2_1	204	206	PF00082	0.456
CLV_PCSK_KEX2_1	224	226	PF00082	0.518
CLV_PCSK_KEX2_1	335	337	PF00082	0.506
CLV_PCSK_PC1ET2_1	117	119	PF00082	0.420
CLV_PCSK_PC1ET2_1	122	124	PF00082	0.482
CLV_PCSK_PC1ET2_1	204	206	PF00082	0.456
CLV_PCSK_PC7_1	118	124	PF00082	0.319
CLV_PCSK_SKI1_1	204	208	PF00082	0.440
CLV_PCSK_SKI1_1	335	339	PF00082	0.416
CLV_PCSK_SKI1_1	473	477	PF00082	0.334
CLV_Separin_Metazoa	188	192	PF03568	0.563
DEG_APCC_DBOX_1	334	342	PF00400	0.413
DOC_CKS1_1	239	244	PF01111	0.510
DOC_CYCLIN_RxL_1	332	342	PF00134	0.413
DOC_MAPK_MEF2A_6	268	276	PF00069	0.406
DOC_MAPK_MEF2A_6	359	366	PF00069	0.406
DOC_PP1_RVXF_1	202	209	PF00149	0.459
DOC_PP1_RVXF_1	471	477	PF00149	0.491
DOC_PP2B_LxvP_1	413	416	PF13499	0.475
DOC_PP2B_LxvP_1	532	535	PF13499	0.580
DOC_PP2B_PxIxI_1	361	367	PF00149	0.344
DOC_USP7_MATH_1	142	146	PF00917	0.417
DOC_USP7_MATH_1	43	47	PF00917	0.394
DOC_USP7_MATH_1	94	98	PF00917	0.556
DOC_WW_Pin1_4	235	240	PF00397	0.540
DOC_WW_Pin1_4	296	301	PF00397	0.559
DOC_WW_Pin1_4	485	490	PF00397	0.491
DOC_WW_Pin1_4	52	57	PF00397	0.357
DOC_WW_Pin1_4	90	95	PF00397	0.715
LIG_14-3-3_CanoR_1	19	28	PF00244	0.489
LIG_APCC_ABBAyCdc20_2	204	210	PF00400	0.480
LIG_BIR_II_1	1	5	PF00653	0.684
LIG_BIR_III_2	186	190	PF00653	0.554
LIG_BRCT_BRCA1_1	255	259	PF00533	0.392
LIG_BRCT_BRCA1_1	303	307	PF00533	0.355
LIG_BRCT_BRCA1_1	343	347	PF00533	0.527
LIG_BRCT_BRCA1_2	255	261	PF00533	0.375
LIG_EVH1_2	526	530	PF00568	0.357
LIG_FHA_1	16	22	PF00498	0.426
LIG_FHA_1	238	244	PF00498	0.582
LIG_FHA_1	31	37	PF00498	0.512
LIG_FHA_1	317	323	PF00498	0.547
LIG_FHA_1	356	362	PF00498	0.437
LIG_FHA_1	409	415	PF00498	0.327
LIG_FHA_1	446	452	PF00498	0.531
LIG_FHA_1	485	491	PF00498	0.479
LIG_FHA_1	529	535	PF00498	0.431
LIG_FHA_2	216	222	PF00498	0.439
LIG_FHA_2	239	245	PF00498	0.538
LIG_FHA_2	57	63	PF00498	0.430
LIG_GBD_Chelix_1	148	156	PF00786	0.336
LIG_LIR_Apic_2	5	10	PF02991	0.525
LIG_LIR_Gen_1	110	121	PF02991	0.406
LIG_LIR_Gen_1	244	252	PF02991	0.522
LIG_LIR_Gen_1	304	314	PF02991	0.342
LIG_LIR_Gen_1	65	70	PF02991	0.352
LIG_LIR_Nem_3	110	116	PF02991	0.314
LIG_LIR_Nem_3	244	249	PF02991	0.544
LIG_LIR_Nem_3	304	310	PF02991	0.353
LIG_LIR_Nem_3	375	381	PF02991	0.441
LIG_LIR_Nem_3	45	51	PF02991	0.467
LIG_LIR_Nem_3	461	466	PF02991	0.559
LIG_LIR_Nem_3	60	66	PF02991	0.343
LIG_SH2_CRK	113	117	PF00017	0.450
LIG_SH2_CRK	387	391	PF00017	0.497
LIG_SH2_CRK	399	403	PF00017	0.390
LIG_SH2_CRK	7	11	PF00017	0.521
LIG_SH2_NCK_1	387	391	PF00017	0.389
LIG_SH2_PTP2	66	69	PF00017	0.455
LIG_SH2_STAP1	387	391	PF00017	0.389
LIG_SH2_STAT5	105	108	PF00017	0.320
LIG_SH2_STAT5	119	122	PF00017	0.396
LIG_SH2_STAT5	466	469	PF00017	0.564
LIG_SH2_STAT5	66	69	PF00017	0.363
LIG_SH3_3	236	242	PF00018	0.509
LIG_SH3_3	244	250	PF00018	0.475
LIG_SH3_3	271	277	PF00018	0.396
LIG_SH3_3	411	417	PF00018	0.438
LIG_SH3_3	483	489	PF00018	0.512
LIG_SH3_3	501	507	PF00018	0.598
LIG_SH3_3	516	522	PF00018	0.676
LIG_SUMO_SIM_par_1	214	221	PF11976	0.407
LIG_SUMO_SIM_par_1	487	494	PF11976	0.459
LIG_SUMO_SIM_par_1	49	55	PF11976	0.382
LIG_TRFH_1	246	250	PF08558	0.360
LIG_TYR_ITIM	385	390	PF00017	0.394
LIG_UBA3_1	393	398	PF00899	0.392
LIG_WW_3	188	192	PF00397	0.563
MOD_CK1_1	138	144	PF00069	0.475
MOD_CK1_1	211	217	PF00069	0.304
MOD_CK1_1	238	244	PF00069	0.475
MOD_CK1_1	340	346	PF00069	0.550
MOD_CK1_1	418	424	PF00069	0.636
MOD_CK2_1	215	221	PF00069	0.475
MOD_CK2_1	238	244	PF00069	0.675
MOD_CK2_1	315	321	PF00069	0.411
MOD_GlcNHglycan	129	132	PF01048	0.565
MOD_GlcNHglycan	133	136	PF01048	0.666
MOD_GlcNHglycan	137	140	PF01048	0.560
MOD_GlcNHglycan	144	147	PF01048	0.527
MOD_GlcNHglycan	234	238	PF01048	0.657
MOD_GlcNHglycan	343	346	PF01048	0.575
MOD_GlcNHglycan	421	424	PF01048	0.575
MOD_GlcNHglycan	480	483	PF01048	0.331
MOD_GlcNHglycan	508	511	PF01048	0.678
MOD_GSK3_1	127	134	PF00069	0.559
MOD_GSK3_1	138	145	PF00069	0.447
MOD_GSK3_1	159	166	PF00069	0.502
MOD_GSK3_1	211	218	PF00069	0.368
MOD_GSK3_1	233	240	PF00069	0.655
MOD_GSK3_1	296	303	PF00069	0.477
MOD_GSK3_1	30	37	PF00069	0.710
MOD_GSK3_1	337	344	PF00069	0.535
MOD_GSK3_1	415	422	PF00069	0.612
MOD_GSK3_1	478	485	PF00069	0.357
MOD_GSK3_1	52	59	PF00069	0.413
MOD_GSK3_1	90	97	PF00069	0.494
MOD_NEK2_1	147	152	PF00069	0.503
MOD_NEK2_1	208	213	PF00069	0.319
MOD_NEK2_1	337	342	PF00069	0.599
MOD_NEK2_2	43	48	PF00069	0.394
MOD_PIKK_1	408	414	PF00454	0.333
MOD_PIKK_1	464	470	PF00454	0.348
MOD_PIKK_1	9	15	PF00454	0.614
MOD_PKA_2	426	432	PF00069	0.613
MOD_PKB_1	231	239	PF00069	0.673
MOD_Plk_1	300	306	PF00069	0.484
MOD_Plk_1	43	49	PF00069	0.458
MOD_Plk_4	215	221	PF00069	0.445
MOD_Plk_4	3	9	PF00069	0.546
MOD_Plk_4	43	49	PF00069	0.442
MOD_Plk_4	525	531	PF00069	0.400
MOD_Plk_4	62	68	PF00069	0.236
MOD_ProDKin_1	235	241	PF00069	0.535
MOD_ProDKin_1	296	302	PF00069	0.549
MOD_ProDKin_1	485	491	PF00069	0.348
MOD_ProDKin_1	52	58	PF00069	0.354
MOD_ProDKin_1	90	96	PF00069	0.703
TRG_DiLeu_BaEn_1	244	249	PF01217	0.388
TRG_DiLeu_BaLyEn_6	202	207	PF01217	0.420
TRG_DiLeu_BaLyEn_6	333	338	PF01217	0.340
TRG_ENDOCYTIC_2	113	116	PF00928	0.335
TRG_ENDOCYTIC_2	387	390	PF00928	0.510
TRG_ENDOCYTIC_2	399	402	PF00928	0.398
TRG_ENDOCYTIC_2	66	69	PF00928	0.375
TRG_ER_diArg_1	190	193	PF00400	0.662
TRG_ER_diArg_1	223	226	PF00400	0.445
TRG_ER_diArg_1	230	233	PF00400	0.576
TRG_ER_diArg_1	333	336	PF00400	0.393
TRG_ER_diArg_1	74	77	PF00400	0.496
TRG_NES_CRM1_1	284	298	PF08389	0.449
TRG_Pf-PMV_PEXEL_1	205	210	PF00026	0.362
TRG_Pf-PMV_PEXEL_1	335	339	PF00026	0.446

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PFZ8	Leptomonas seymouri	43%	100%
A0A1X0NMY7	Trypanosomatidae	30%	100%
A0A422N0R9	Trypanosoma rangeli	27%	100%
A4H7A7	Leishmania braziliensis	70%	99%
A4HVQ2	Leishmania infantum	99%	99%
D0A6I9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	100%
E9APE8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	99%
Q4QG30	Leishmania major	88%	100%
V5ARR9	Trypanosoma cruzi	26%	100%