DNA-directed RNA polymerase 2, RPB12 subunit, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA polymerase subunit, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IBK2_LEIDO

TriTrypDb:

LdBPK_200560.1 , LdCL_200010300 , LDHU3_20.0660

Length:

205

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 3
NetGPI	no	yes: 0, no: 3

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	2
GO:0030880	RNA polymerase complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1990234	transferase complex	3	2

Expansion

Sequence features

A0A3Q8IBK2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IBK2

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006351	DNA-templated transcription	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0032774	RNA biosynthetic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:0097659	nucleic acid-templated transcription	6	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	4
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	4
GO:0016740	transferase activity	2	4
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	4
GO:0016779	nucleotidyltransferase activity	4	4
GO:0034062	5'-3' RNA polymerase activity	5	4
GO:0097747	RNA polymerase activity	4	4
GO:0140098	catalytic activity, acting on RNA	3	4
GO:0140640	catalytic activity, acting on a nucleic acid	2	4
GO:0003676	nucleic acid binding	3	1
GO:0003677	DNA binding	4	1
GO:0005488	binding	1	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	108	110	PF00675	0.600
CLV_PCSK_KEX2_1	108	110	PF00082	0.600
DOC_MAPK_gen_1	108	116	PF00069	0.596
DOC_MAPK_gen_1	152	161	PF00069	0.558
DOC_MAPK_MEF2A_6	17	24	PF00069	0.465
DOC_MAPK_NFAT4_5	17	25	PF00069	0.466
DOC_USP7_MATH_1	11	15	PF00917	0.461
DOC_USP7_MATH_1	16	20	PF00917	0.476
DOC_USP7_MATH_1	180	184	PF00917	0.600
DOC_USP7_MATH_1	58	62	PF00917	0.458
DOC_USP7_MATH_1	71	75	PF00917	0.559
DOC_USP7_UBL2_3	117	121	PF12436	0.586
LIG_14-3-3_CanoR_1	12	16	PF00244	0.451
LIG_14-3-3_CanoR_1	17	21	PF00244	0.458
LIG_14-3-3_CanoR_1	59	64	PF00244	0.539
LIG_BRCT_BRCA1_1	188	192	PF00533	0.506
LIG_BRCT_BRCA1_2	188	194	PF00533	0.498
LIG_FHA_1	101	107	PF00498	0.775
LIG_FHA_1	156	162	PF00498	0.538
LIG_FHA_1	164	170	PF00498	0.564
LIG_FHA_1	36	42	PF00498	0.508
LIG_FHA_2	144	150	PF00498	0.597
LIG_FHA_2	198	204	PF00498	0.569
LIG_LIR_Gen_1	200	205	PF02991	0.579
LIG_LIR_Nem_3	189	195	PF02991	0.492
LIG_LIR_Nem_3	200	205	PF02991	0.514
LIG_PDZ_Class_3	200	205	PF00595	0.579
LIG_SH2_STAT5	160	163	PF00017	0.462
LIG_SH2_STAT5	170	173	PF00017	0.392
LIG_SH3_3	15	21	PF00018	0.459
LIG_SH3_3	65	71	PF00018	0.552
LIG_TRAF2_1	132	135	PF00917	0.587
LIG_TRAF2_1	142	145	PF00917	0.538
LIG_TYR_ITSM	198	205	PF00017	0.573
MOD_CK1_1	183	189	PF00069	0.680
MOD_CK1_1	27	33	PF00069	0.483
MOD_CK1_1	62	68	PF00069	0.478
MOD_GlcNHglycan	111	114	PF01048	0.721
MOD_GlcNHglycan	64	67	PF01048	0.541
MOD_GSK3_1	16	23	PF00069	0.462
MOD_GSK3_1	183	190	PF00069	0.612
MOD_GSK3_1	194	201	PF00069	0.662
MOD_GSK3_1	27	34	PF00069	0.470
MOD_GSK3_1	58	65	PF00069	0.587
MOD_N-GLC_1	163	168	PF02516	0.458
MOD_NEK2_1	24	29	PF00069	0.534
MOD_NEK2_2	187	192	PF00069	0.568
MOD_OFUCOSY	4	10	PF10250	0.447
MOD_PIKK_1	130	136	PF00454	0.593
MOD_PIKK_1	140	146	PF00454	0.536
MOD_PIKK_1	49	55	PF00454	0.448
MOD_PKA_2	11	17	PF00069	0.450
MOD_PKA_2	58	64	PF00069	0.540
MOD_PKB_1	57	65	PF00069	0.464
MOD_Plk_2-3	198	204	PF00069	0.569
MOD_Plk_4	155	161	PF00069	0.464
MOD_Plk_4	187	193	PF00069	0.506
MOD_Plk_4	20	26	PF00069	0.466
MOD_Plk_4	59	65	PF00069	0.462
MOD_SUMO_for_1	116	119	PF00179	0.591
MOD_SUMO_for_1	120	123	PF00179	0.541
TRG_ENDOCYTIC_2	202	205	PF00928	0.588
TRG_ER_diArg_1	108	110	PF00400	0.561
TRG_ER_diArg_1	56	59	PF00400	0.468

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A4HYR2	Leishmania infantum	99%	100%
Q4QCZ1	Leishmania major	88%	100%