Hypothetical_protein_conserved

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GRAM domain containing protein, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IAW1_LEIDO

TriTrypDb:

LdBPK_170750.1 , LdCL_170013100 , LDHU3_17.1080

Length:

352

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0005886	plasma membrane	3	1
GO:0031090	organelle membrane	3	1
GO:0044232	organelle membrane contact site	2	1
GO:0140268	endoplasmic reticulum-plasma membrane contact site	3	1

Expansion

Sequence features

A0A3Q8IAW1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IAW1

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006869	lipid transport	5	1
GO:0009987	cellular process	1	1
GO:0015850	organic hydroxy compound transport	5	1
GO:0015918	sterol transport	6	1
GO:0032365	intracellular lipid transport	4	1
GO:0032366	intracellular sterol transport	5	1
GO:0046907	intracellular transport	3	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0071702	organic substance transport	4	1

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	1
GO:0005319	lipid transporter activity	2	1
GO:0005488	binding	1	1
GO:0005496	steroid binding	3	1
GO:0008289	lipid binding	2	1
GO:0015248	sterol transporter activity	3	1
GO:0032934	sterol binding	4	1
GO:0097159	organic cyclic compound binding	2	1
GO:0120013	lipid transfer activity	3	1
GO:0120015	sterol transfer activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	201	205	PF00656	0.603
CLV_C14_Caspase3-7	304	308	PF00656	0.460
CLV_NRD_NRD_1	258	260	PF00675	0.427
CLV_NRD_NRD_1	78	80	PF00675	0.246
CLV_PCSK_KEX2_1	258	260	PF00082	0.438
CLV_PCSK_SKI1_1	102	106	PF00082	0.298
CLV_PCSK_SKI1_1	125	129	PF00082	0.257
CLV_PCSK_SKI1_1	325	329	PF00082	0.475
CLV_PCSK_SKI1_1	55	59	PF00082	0.284
DEG_APCC_DBOX_1	124	132	PF00400	0.516
DOC_CKS1_1	45	50	PF01111	0.562
DOC_CYCLIN_yCln2_LP_2	45	51	PF00134	0.498
DOC_MAPK_MEF2A_6	130	139	PF00069	0.503
DOC_USP7_MATH_1	17	21	PF00917	0.594
DOC_USP7_MATH_1	218	222	PF00917	0.647
DOC_USP7_MATH_1	289	293	PF00917	0.517
DOC_WW_Pin1_4	241	246	PF00397	0.805
DOC_WW_Pin1_4	44	49	PF00397	0.615
LIG_14-3-3_CanoR_1	102	110	PF00244	0.457
LIG_14-3-3_CanoR_1	192	202	PF00244	0.498
LIG_14-3-3_CanoR_1	288	294	PF00244	0.482
LIG_Actin_WH2_2	264	280	PF00022	0.471
LIG_AP2alpha_1	150	154	PF02296	0.489
LIG_BIR_II_1	1	5	PF00653	0.584
LIG_CaM_NSCaTE_8	317	324	PF13499	0.438
LIG_deltaCOP1_diTrp_1	83	89	PF00928	0.535
LIG_eIF4E_1	123	129	PF01652	0.516
LIG_FHA_1	105	111	PF00498	0.460
LIG_FHA_1	134	140	PF00498	0.451
LIG_FHA_1	263	269	PF00498	0.447
LIG_FHA_1	281	287	PF00498	0.313
LIG_FHA_1	67	73	PF00498	0.546
LIG_FHA_2	234	240	PF00498	0.771
LIG_FHA_2	4	10	PF00498	0.673
LIG_IRF3_LxIS_1	330	337	PF10401	0.424
LIG_LIR_Gen_1	22	31	PF02991	0.550
LIG_LIR_Gen_1	279	289	PF02991	0.657
LIG_LIR_Gen_1	36	46	PF02991	0.535
LIG_LIR_Nem_3	122	126	PF02991	0.464
LIG_LIR_Nem_3	22	27	PF02991	0.574
LIG_LIR_Nem_3	279	284	PF02991	0.579
LIG_LIR_Nem_3	316	320	PF02991	0.456
LIG_LIR_Nem_3	337	342	PF02991	0.482
LIG_LIR_Nem_3	343	347	PF02991	0.488
LIG_LIR_Nem_3	36	41	PF02991	0.533
LIG_MYND_1	248	252	PF01753	0.701
LIG_PDZ_Class_1	347	352	PF00595	0.616
LIG_Pex14_2	150	154	PF04695	0.489
LIG_Pex14_2	61	65	PF04695	0.471
LIG_SH2_SRC	118	121	PF00017	0.463
LIG_SH2_STAT5	120	123	PF00017	0.531
LIG_SH2_STAT5	180	183	PF00017	0.517
LIG_SH2_STAT5	332	335	PF00017	0.441
LIG_SH3_1	242	248	PF00018	0.682
LIG_SH3_1	85	91	PF00018	0.498
LIG_SH3_2	88	93	PF14604	0.476
LIG_SH3_3	242	248	PF00018	0.738
LIG_SH3_3	85	91	PF00018	0.498
LIG_SUMO_SIM_par_1	282	287	PF11976	0.414
LIG_SUMO_SIM_par_1	332	337	PF11976	0.393
LIG_TYR_ITIM	330	335	PF00017	0.364
LIG_WRC_WIRS_1	159	164	PF05994	0.476
MOD_CDK_SPxK_1	44	50	PF00069	0.562
MOD_CK1_1	167	173	PF00069	0.536
MOD_CK1_1	233	239	PF00069	0.725
MOD_CK1_1	33	39	PF00069	0.559
MOD_CK2_1	22	28	PF00069	0.566
MOD_CK2_1	233	239	PF00069	0.677
MOD_CK2_1	287	293	PF00069	0.413
MOD_CK2_1	3	9	PF00069	0.620
MOD_CMANNOS	86	89	PF00535	0.291
MOD_GlcNHglycan	131	134	PF01048	0.319
MOD_GlcNHglycan	166	169	PF01048	0.346
MOD_GlcNHglycan	220	223	PF01048	0.497
MOD_GlcNHglycan	289	292	PF01048	0.654
MOD_GlcNHglycan	32	35	PF01048	0.411
MOD_GlcNHglycan	321	324	PF01048	0.680
MOD_GSK3_1	129	136	PF00069	0.542
MOD_GSK3_1	160	167	PF00069	0.538
MOD_GSK3_1	193	200	PF00069	0.510
MOD_GSK3_1	22	29	PF00069	0.560
MOD_GSK3_1	276	283	PF00069	0.380
MOD_NEK2_1	104	109	PF00069	0.498
MOD_NEK2_1	129	134	PF00069	0.552
MOD_NEK2_1	183	188	PF00069	0.504
MOD_NEK2_1	284	289	PF00069	0.395
MOD_NEK2_1	3	8	PF00069	0.598
MOD_NEK2_1	334	339	PF00069	0.366
MOD_NEK2_2	171	176	PF00069	0.503
MOD_NEK2_2	340	345	PF00069	0.329
MOD_PIKK_1	140	146	PF00454	0.513
MOD_PIKK_1	160	166	PF00454	0.345
MOD_PIKK_1	22	28	PF00454	0.643
MOD_PKA_1	79	85	PF00069	0.457
MOD_PKA_2	287	293	PF00069	0.508
MOD_PKA_2	300	306	PF00069	0.376
MOD_PKA_2	89	95	PF00069	0.524
MOD_Plk_1	171	177	PF00069	0.519
MOD_Plk_1	36	42	PF00069	0.541
MOD_Plk_4	105	111	PF00069	0.480
MOD_Plk_4	183	189	PF00069	0.476
MOD_Plk_4	251	257	PF00069	0.665
MOD_Plk_4	276	282	PF00069	0.360
MOD_ProDKin_1	241	247	PF00069	0.801
MOD_ProDKin_1	44	50	PF00069	0.619
MOD_SUMO_rev_2	73	82	PF00179	0.513
MOD_SUMO_rev_2	8	16	PF00179	0.603
TRG_DiLeu_BaLyEn_6	245	250	PF01217	0.622
TRG_ENDOCYTIC_2	118	121	PF00928	0.498
TRG_ENDOCYTIC_2	126	129	PF00928	0.433
TRG_ENDOCYTIC_2	180	183	PF00928	0.517
TRG_ENDOCYTIC_2	332	335	PF00928	0.441
TRG_ER_diArg_1	258	260	PF00400	0.627
TRG_NES_CRM1_1	304	316	PF08389	0.395

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4JTD3	Bodo saltans	26%	82%
A0A1X0NTC3	Trypanosomatidae	53%	99%
A0A3R7N4K3	Trypanosoma rangeli	42%	100%
A0A3R7RDH3	Trypanosoma rangeli	45%	100%
A0A422NZS9	Trypanosoma rangeli	25%	92%
A4H910	Leishmania braziliensis	80%	100%
A4HXE2	Leishmania infantum	100%	100%
E9AR37	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
Q4QEC6	Leishmania major	93%	100%
V5BE10	Trypanosoma cruzi	45%	99%