Cleavage and polyadenylation specificity factor 30 kDa subunit, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cleavage and polyadenylation specificity factor 30 kDa subunit, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IAT5_LEIDO

TriTrypDb:

LdBPK_090770.1 , LdCL_090013100 , LDHU3_09.0920

Length:

340

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005847	mRNA cleavage and polyadenylation specificity factor complex	4	1
GO:0005849	mRNA cleavage factor complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A0A3Q8IAT5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IAT5

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006379	mRNA cleavage	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	12
GO:0090501	RNA phosphodiester bond hydrolysis	6	12
GO:0098787	mRNA cleavage involved in mRNA processing	8	12
GO:0098789	pre-mRNA cleavage required for polyadenylation	9	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006396	RNA processing	6	1
GO:0006397	mRNA processing	7	1
GO:0031426	polycistronic mRNA processing	8	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0005488	binding	1	12
GO:0008270	zinc ion binding	6	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0046914	transition metal ion binding	5	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	122	124	PF00675	0.358
CLV_NRD_NRD_1	250	252	PF00675	0.263
CLV_NRD_NRD_1	298	300	PF00675	0.599
CLV_NRD_NRD_1	301	303	PF00675	0.594
CLV_NRD_NRD_1	333	335	PF00675	0.695
CLV_PCSK_FUR_1	299	303	PF00082	0.709
CLV_PCSK_KEX2_1	122	124	PF00082	0.365
CLV_PCSK_KEX2_1	138	140	PF00082	0.392
CLV_PCSK_KEX2_1	174	176	PF00082	0.532
CLV_PCSK_KEX2_1	250	252	PF00082	0.387
CLV_PCSK_KEX2_1	297	299	PF00082	0.596
CLV_PCSK_KEX2_1	301	303	PF00082	0.591
CLV_PCSK_KEX2_1	332	334	PF00082	0.701
CLV_PCSK_PC1ET2_1	138	140	PF00082	0.517
CLV_PCSK_PC1ET2_1	174	176	PF00082	0.524
CLV_PCSK_PC7_1	297	303	PF00082	0.711
CLV_PCSK_SKI1_1	135	139	PF00082	0.363
CLV_PCSK_SKI1_1	174	178	PF00082	0.512
CLV_PCSK_SKI1_1	192	196	PF00082	0.373
CLV_PCSK_SKI1_1	251	255	PF00082	0.384
DEG_Nend_Nbox_1	1	3	PF02207	0.467
DOC_MAPK_gen_1	174	182	PF00069	0.386
DOC_USP7_MATH_1	111	115	PF00917	0.468
DOC_USP7_MATH_1	214	218	PF00917	0.520
DOC_USP7_MATH_1	285	289	PF00917	0.710
LIG_14-3-3_CanoR_1	48	57	PF00244	0.478
LIG_Actin_WH2_2	54	69	PF00022	0.331
LIG_BIR_III_2	43	47	PF00653	0.506
LIG_BIR_III_4	5	9	PF00653	0.546
LIG_FHA_1	231	237	PF00498	0.692
LIG_FHA_2	259	265	PF00498	0.367
LIG_FHA_2	7	13	PF00498	0.541
LIG_FHA_2	98	104	PF00498	0.415
LIG_LIR_Nem_3	52	57	PF02991	0.503
LIG_PCNA_PIPBox_1	58	67	PF02747	0.335
LIG_REV1ctd_RIR_1	90	99	PF16727	0.506
LIG_SH2_CRK	54	58	PF00017	0.497
LIG_SH2_GRB2like	82	85	PF00017	0.480
LIG_SH2_STAP1	167	171	PF00017	0.447
LIG_SH2_STAP1	82	86	PF00017	0.331
LIG_SH2_STAT3	86	89	PF00017	0.450
LIG_SH2_STAT5	146	149	PF00017	0.429
LIG_SH2_STAT5	78	81	PF00017	0.241
LIG_SH2_STAT5	86	89	PF00017	0.306
LIG_SH3_3	124	130	PF00018	0.345
LIG_SH3_3	149	155	PF00018	0.438
LIG_SH3_3	206	212	PF00018	0.387
LIG_SH3_3	226	232	PF00018	0.293
LIG_SH3_3	279	285	PF00018	0.765
LIG_SH3_3	99	105	PF00018	0.404
LIG_TRAF2_1	162	165	PF00917	0.438
MOD_CK2_1	111	117	PF00069	0.370
MOD_CK2_1	97	103	PF00069	0.404
MOD_Cter_Amidation	330	333	PF01082	0.635
MOD_GlcNHglycan	140	143	PF01048	0.558
MOD_GlcNHglycan	192	195	PF01048	0.431
MOD_GlcNHglycan	237	240	PF01048	0.718
MOD_GlcNHglycan	287	290	PF01048	0.722
MOD_GlcNHglycan	312	315	PF01048	0.739
MOD_GlcNHglycan	51	54	PF01048	0.444
MOD_GSK3_1	186	193	PF00069	0.523
MOD_GSK3_1	49	56	PF00069	0.369
MOD_LATS_1	136	142	PF00433	0.492
MOD_N-GLC_1	111	116	PF02516	0.457
MOD_N-GLC_1	190	195	PF02516	0.373
MOD_NEK2_1	106	111	PF00069	0.594
MOD_NEK2_1	13	18	PF00069	0.463
MOD_NEK2_1	190	195	PF00069	0.495
MOD_PIKK_1	111	117	PF00454	0.446
MOD_PKA_1	138	144	PF00069	0.489
MOD_PKA_2	138	144	PF00069	0.441
MOD_Plk_1	13	19	PF00069	0.511
MOD_Plk_2-3	258	264	PF00069	0.384
MOD_Plk_4	214	220	PF00069	0.516
MOD_SUMO_for_1	137	140	PF00179	0.494
MOD_SUMO_rev_2	20	28	PF00179	0.582
TRG_ENDOCYTIC_2	54	57	PF00928	0.502
TRG_ER_diArg_1	121	123	PF00400	0.345
TRG_ER_diArg_1	297	299	PF00400	0.690
TRG_ER_diArg_1	300	302	PF00400	0.693
TRG_ER_diArg_1	332	334	PF00400	0.701
TRG_ER_diArg_1	38	41	PF00400	0.506
TRG_Pf-PMV_PEXEL_1	221	225	PF00026	0.552
TRG_Pf-PMV_PEXEL_1	298	303	PF00026	0.644

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEE4	Leptomonas seymouri	87%	100%
A0A0S4KP33	Bodo saltans	50%	99%
A0A1X0NMX4	Trypanosomatidae	59%	100%
A0A3R7KCZ6	Trypanosoma rangeli	60%	100%
A4H5Q3	Leishmania braziliensis	97%	100%
A4HTZ3	Leishmania infantum	100%	100%
D0A9F4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	100%
E9AMS3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
O95639	Homo sapiens	33%	100%
Q4QHV2	Leishmania major	98%	100%
V5D8L4	Trypanosoma cruzi	65%	100%