Asparaginase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Asparaginase

Gene product:

cytoplasmic l-asparaginase i-like protein

Species:

Leishmania donovani

UniProt:

A0A3Q8IA09_LEIDO

TriTrypDb:

LdBPK_150440.1 , LdCL_150009300 , LDHU3_15.0540

Length:

398

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3Q8IA09

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IA09

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	11
GO:0006520	amino acid metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009066	aspartate family amino acid metabolic process	5	11
GO:0009987	cellular process	1	11
GO:0019752	carboxylic acid metabolic process	5	11
GO:0043436	oxoacid metabolic process	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044281	small molecule metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901605	alpha-amino acid metabolic process	4	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004067	asparaginase activity	5	11
GO:0016787	hydrolase activity	2	11
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	3	11
GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	4	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	277	281	PF00656	0.379
CLV_C14_Caspase3-7	82	86	PF00656	0.336
CLV_NRD_NRD_1	155	157	PF00675	0.312
CLV_NRD_NRD_1	28	30	PF00675	0.327
CLV_NRD_NRD_1	309	311	PF00675	0.401
CLV_PCSK_KEX2_1	155	157	PF00082	0.308
CLV_PCSK_KEX2_1	259	261	PF00082	0.424
CLV_PCSK_KEX2_1	28	30	PF00082	0.331
CLV_PCSK_KEX2_1	309	311	PF00082	0.370
CLV_PCSK_PC1ET2_1	259	261	PF00082	0.424
CLV_PCSK_SKI1_1	28	32	PF00082	0.312
CLV_PCSK_SKI1_1	284	288	PF00082	0.410
DEG_Nend_UBRbox_2	1	3	PF02207	0.668
DEG_SCF_FBW7_2	388	393	PF00400	0.536
DOC_ANK_TNKS_1	155	162	PF00023	0.410
DOC_CYCLIN_RxL_1	281	288	PF00134	0.410
DOC_CYCLIN_yCln2_LP_2	134	140	PF00134	0.312
DOC_CYCLIN_yCln2_LP_2	221	227	PF00134	0.518
DOC_MAPK_gen_1	22	32	PF00069	0.475
DOC_MAPK_MEF2A_6	155	162	PF00069	0.312
DOC_MAPK_MEF2A_6	169	177	PF00069	0.312
DOC_MAPK_MEF2A_6	198	207	PF00069	0.457
DOC_MAPK_MEF2A_6	28	35	PF00069	0.317
DOC_MAPK_MEF2A_6	385	394	PF00069	0.374
DOC_MAPK_NFAT4_5	28	36	PF00069	0.331
DOC_USP7_MATH_1	12	16	PF00917	0.668
DOC_USP7_MATH_1	196	200	PF00917	0.424
DOC_USP7_MATH_1	272	276	PF00917	0.388
DOC_WW_Pin1_4	261	266	PF00397	0.350
DOC_WW_Pin1_4	342	347	PF00397	0.331
DOC_WW_Pin1_4	369	374	PF00397	0.351
DOC_WW_Pin1_4	386	391	PF00397	0.345
DOC_WW_Pin1_4	7	12	PF00397	0.677
LIG_14-3-3_CanoR_1	260	265	PF00244	0.418
LIG_Actin_WH2_2	293	311	PF00022	0.401
LIG_Actin_WH2_2	53	69	PF00022	0.457
LIG_APCC_ABBA_1	101	106	PF00400	0.457
LIG_FHA_1	209	215	PF00498	0.470
LIG_FHA_1	25	31	PF00498	0.516
LIG_FHA_1	379	385	PF00498	0.519
LIG_FHA_1	387	393	PF00498	0.530
LIG_GBD_Chelix_1	325	333	PF00786	0.379
LIG_GBD_Chelix_1	52	60	PF00786	0.366
LIG_LIR_Gen_1	250	256	PF02991	0.340
LIG_LIR_Gen_1	79	84	PF02991	0.300
LIG_LIR_Gen_1	93	101	PF02991	0.247
LIG_LIR_Nem_3	106	111	PF02991	0.356
LIG_LIR_Nem_3	193	197	PF02991	0.322
LIG_LIR_Nem_3	199	205	PF02991	0.311
LIG_LIR_Nem_3	250	255	PF02991	0.340
LIG_LIR_Nem_3	79	83	PF02991	0.300
LIG_LIR_Nem_3	93	97	PF02991	0.247
LIG_LYPXL_yS_3	202	205	PF13949	0.331
LIG_NRBOX	126	132	PF00104	0.331
LIG_SH2_CRK	94	98	PF00017	0.385
LIG_SH2_GRB2like	350	353	PF00017	0.457
LIG_SH2_STAP1	122	126	PF00017	0.312
LIG_SH2_STAP1	80	84	PF00017	0.312
LIG_SH2_STAT5	122	125	PF00017	0.328
LIG_SH2_STAT5	34	37	PF00017	0.352
LIG_SH2_STAT5	350	353	PF00017	0.329
LIG_SH2_STAT5	365	368	PF00017	0.278
LIG_SH2_STAT5	55	58	PF00017	0.311
LIG_SH2_STAT5	94	97	PF00017	0.331
LIG_SH3_3	11	17	PF00018	0.710
LIG_SH3_3	239	245	PF00018	0.463
LIG_SH3_CIN85_PxpxPR_1	17	22	PF14604	0.523
LIG_SUMO_SIM_anti_2	250	257	PF11976	0.388
LIG_SUMO_SIM_par_1	136	141	PF11976	0.329
LIG_SUMO_SIM_par_1	143	149	PF11976	0.328
LIG_TYR_ITIM	200	205	PF00017	0.457
LIG_TYR_ITIM	78	83	PF00017	0.312
LIG_UBA3_1	130	136	PF00899	0.310
LIG_UBA3_1	363	369	PF00899	0.331
LIG_WRC_WIRS_1	249	254	PF05994	0.340
MOD_CDC14_SPxK_1	372	375	PF00782	0.457
MOD_CDK_SPxK_1	369	375	PF00069	0.457
MOD_CK1_1	125	131	PF00069	0.355
MOD_CK2_1	354	360	PF00069	0.356
MOD_GlcNHglycan	124	127	PF01048	0.355
MOD_GlcNHglycan	328	332	PF01048	0.370
MOD_GlcNHglycan	356	359	PF01048	0.410
MOD_GSK3_1	116	123	PF00069	0.312
MOD_GSK3_1	268	275	PF00069	0.432
MOD_GSK3_1	329	336	PF00069	0.367
MOD_N-GLC_1	177	182	PF02516	0.397
MOD_N-GLC_2	185	187	PF02516	0.356
MOD_NEK2_1	120	125	PF00069	0.312
MOD_NEK2_1	163	168	PF00069	0.357
MOD_NEK2_1	177	182	PF00069	0.245
MOD_NEK2_1	268	273	PF00069	0.282
MOD_NEK2_1	35	40	PF00069	0.421
MOD_NEK2_1	354	359	PF00069	0.310
MOD_NEK2_2	116	121	PF00069	0.312
MOD_PIKK_1	378	384	PF00454	0.511
MOD_PKA_2	24	30	PF00069	0.520
MOD_PKA_2	66	72	PF00069	0.457
MOD_Plk_1	177	183	PF00069	0.397
MOD_Plk_4	125	131	PF00069	0.312
MOD_Plk_4	177	183	PF00069	0.424
MOD_Plk_4	248	254	PF00069	0.345
MOD_ProDKin_1	261	267	PF00069	0.350
MOD_ProDKin_1	342	348	PF00069	0.331
MOD_ProDKin_1	369	375	PF00069	0.351
MOD_ProDKin_1	386	392	PF00069	0.364
MOD_ProDKin_1	7	13	PF00069	0.679
MOD_SUMO_for_1	215	218	PF00179	0.488
MOD_SUMO_rev_2	357	364	PF00179	0.367
TRG_DiLeu_BaEn_1	79	84	PF01217	0.312
TRG_DiLeu_BaEn_2	106	112	PF01217	0.366
TRG_DiLeu_BaLyEn_6	282	287	PF01217	0.385
TRG_DiLeu_LyEn_5	387	392	PF01217	0.522
TRG_ENDOCYTIC_2	108	111	PF00928	0.312
TRG_ENDOCYTIC_2	194	197	PF00928	0.360
TRG_ENDOCYTIC_2	202	205	PF00928	0.296
TRG_ENDOCYTIC_2	80	83	PF00928	0.312
TRG_ENDOCYTIC_2	94	97	PF00928	0.304
TRG_ER_diArg_1	234	237	PF00400	0.457
TRG_ER_diArg_1	28	30	PF00400	0.326
TRG_ER_diArg_1	308	310	PF00400	0.401
TRG_NLS_MonoCore_2	258	263	PF00514	0.457
TRG_Pf-PMV_PEXEL_1	64	68	PF00026	0.410
TRG_PTS1	395	398	PF00515	0.639

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HVX8	Leptomonas seymouri	74%	100%
A0A0N1PEN4	Leptomonas seymouri	32%	100%
A0A0S4J4T5	Bodo saltans	29%	98%
A0A0S4J8X9	Bodo saltans	33%	100%
A0A0S4JTX6	Bodo saltans	46%	98%
A0A1X0NTQ3	Trypanosomatidae	53%	86%
A0A1X0P1U7	Trypanosomatidae	30%	100%
A0A3R7KPG5	Trypanosoma rangeli	32%	100%
A0A3S7XBI7	Leishmania donovani	30%	100%
A0A422N3G9	Trypanosoma rangeli	51%	88%
A0JNU3	Mus musculus	31%	71%
A1RX40	Thermofilum pendens (strain DSM 2475 / Hrk 5)	25%	88%
A4FWR5	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	26%	95%
A4H811	Leishmania braziliensis	82%	100%
A4HPW0	Leishmania braziliensis	30%	100%
A4HWE1	Leishmania infantum	99%	100%
A4IDM2	Leishmania infantum	30%	100%
A4YHH3	Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)	26%	91%
A5UK11	Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)	27%	91%
A6UPR4	Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)	24%	93%
A6VGK5	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	26%	95%
A9AA46	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	24%	95%
C3MPS1	Sulfolobus islandicus (strain L.S.2.15 / Lassen #1)	27%	89%
C3MYR5	Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)	27%	89%
C3N5E7	Sulfolobus islandicus (strain M.16.27)	27%	89%
C3NE01	Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)	27%	89%
C3NHQ2	Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)	27%	89%
C4KH13	Sulfolobus islandicus (strain M.16.4 / Kamchatka #3)	27%	89%
E9AQ41	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9ATM7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
O26802	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	26%	91%
O59132	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	27%	91%
O88202	Rattus norvegicus	31%	71%
P0A962	Escherichia coli (strain K12)	37%	100%
P0A963	Escherichia coli O157:H7	37%	100%
P61400	Methanococcus maripaludis (strain S2 / LL)	26%	95%
Q18GL3	Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)	24%	90%
Q2NEH1	Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)	25%	91%
Q4J955	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	24%	89%
Q4Q0X1	Leishmania major	30%	100%
Q4QFE1	Leishmania major	92%	100%
Q5JI77	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	25%	90%
Q60331	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	24%	95%
Q86U10	Homo sapiens	32%	69%
Q8PUM7	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	25%	94%
Q8TR66	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	23%	94%
Q8TV84	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	26%	87%
Q8TZE8	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	25%	100%
Q8U0X0	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	24%	91%
Q971W5	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	25%	89%
Q97ZH5	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	27%	90%
Q9U518	Dirofilaria immitis	31%	67%
Q9V0T9	Pyrococcus abyssi (strain GE5 / Orsay)	26%	91%
V5BC16	Trypanosoma cruzi	51%	77%
V5BUU3	Trypanosoma cruzi	29%	100%